Return to search results Title: Etv6-dependent positive and negative gene regulatory network controls vegfa expression in vivo [RNA-seq]

Summary

VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VegfA have been uncovered, vegfa transcriptional regulation in vivo is unclear. Here, we show that ETS transcription factor Etv6 positively regulates vegfa expression during blood stem cell development through multiple transcriptional inputs. In agreement with its established repressive functions, Etv6 directly inhibits expression of vegfa repressor foxo3. Surprisingly, it also directly activates expression of vegfa activator klf4. Finally, it indirectly binds to vegfa promoter by the recruiment of Klf4. Thus, our work uncovers a dual function for Etv6, as both a transcriptional repressor and activator, in controlling a major signaling pathway.

Contributors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-Uitz, Catherine Porcher

Experiment Type: RNA-seq was performed on three independent biological samples obtained from the somites of wild-type (WT) or etv6 morpholino injected stage 22 embryos. Indexed libraries were then generated, pooled and sequenced using a NextSeq 500 sequencer.

Experiment Reagents: etv6 MO1.

Article: XB-ART-55761, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
somite - NF22 GSM3171642  GSM3171643  GSM3171644  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Conditions: Tissue Assay Stages ChIP Antibody somite NF stage 22      somite + etv6 MO - NF22 DEG GSM3171645  GSM3171646  GSM3171647  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection etv6 MO1 etv6 (Xla) NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody somite NF stage 22

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Select All  Xtr    Xla

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