Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2000 Nov 07;9723:12498-502. doi: 10.1073/pnas.220425497.
Show Gene links
Show Anatomy links
Vigilin binding selectively inhibits cleavage of the vitellogenin mRNA 3'-untranslated region by the mRNA endonuclease polysomal ribonuclease 1.
Cunningham KS, Dodson RE, Nagel MA, Shapiro DJ, Schoenberg DR.
???displayArticle.abstract???
In Xenopus, estrogen induces the stabilization of vitellogenin mRNA and the destabilization of albumin mRNA. These processes correlate with increased polysomal activity of a sequence-selective mRNA endonuclease, PMR-1, and a hnRNP K homology-domain RNA-binding protein, vigilin. Vigilin binds to a region of the vitellogenin mRNA 3'-untranslated region (3'-UTR) implicated in estrogen-mediated stabilization. The vigilin-binding site in the vitellogenin B1 mRNA 3'-UTR contains two consensus PMR-1 cleavage sites. The availability of purified PMR-1 and recombinant vigilin made it possible to test the hypothesis that RNA-binding proteins interact with cis-acting elements to stabilize target mRNAs by blocking cleavage by site-specific mRNA endonucleases. Vigilin binds to the vitellogenin mRNA 3'-UTR site with at least 30-fold higher affinity than it exhibits for the albumin mRNA segment containing the mapped PMR-1 cleavage sites. This differential binding affinity correlates with differential in vitro susceptibility of the protein-RNA complexes to cleavage by PMR-1. Whereas recombinant vigilin has no detectable protective effect on PMR-1 cleavage of albumin mRNA, it retards in vitro cleavage of the vitellogenin mRNA 3'-UTR by purified PMR-1. The PMR-1 sites in the vitellogenin mRNA 3'-UTR are functional because they are readily cleaved in vitro by purified PMR-1. These results provide direct evidence for differential susceptibility to endonuclease-mediated mRNA decay resulting from the differential affinity of a RNA-binding protein for cis-acting stability determinants.
Binder,
Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening.
1994, Pubmed
Binder,
Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening.
1994,
Pubmed Binder,
Degradation of apolipoprotein II mRNA occurs via endonucleolytic cleavage at 5'-AAU-3'/5'-UAA-3' elements in single-stranded loop domains of the 3'-noncoding region.
1989,
Pubmed Brock,
Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation.
1983,
Pubmed
,
Xenbase Chernokalskaya,
A polysomal ribonuclease involved in the destabilization of albumin mRNA is a novel member of the peroxidase gene family.
1998,
Pubmed
,
Xenbase Chernokalskaya,
Cleavage properties of an estrogen-regulated polysomal ribonuclease involved in the destabilization of albumin mRNA.
1997,
Pubmed
,
Xenbase Chiang,
Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators.
1995,
Pubmed DeMaria,
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
1996,
Pubmed Dodson,
Vigilin, a ubiquitous protein with 14 K homology domains, is the estrogen-inducible vitellogenin mRNA 3'-untranslated region-binding protein.
1997,
Pubmed
,
Xenbase Dodson,
An estrogen-inducible protein binds specifically to a sequence in the 3' untranslated region of estrogen-stabilized vitellogenin mRNA.
1994,
Pubmed
,
Xenbase Dompenciel,
Purification and characterization of an estrogen-regulated Xenopus liver polysomal nuclease involved in the selective destabilization of albumin mRNA.
1995,
Pubmed
,
Xenbase Doyle,
The c-myc coding region determinant-binding protein: a member of a family of KH domain RNA-binding proteins.
1998,
Pubmed Gallouzi,
A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein: a potential link between signal transduction and RNA stability.
1998,
Pubmed Kanamori,
In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain protein.
1998,
Pubmed
,
Xenbase Lang,
Scp160p, a multiple KH-domain protein, is a component of mRNP complexes in yeast.
2000,
Pubmed Lee,
Purification and characterization of a polysome-associated endoribonuclease that degrades c-myc mRNA in vitro.
1998,
Pubmed McKnight,
Cloning and expression of a cellular high density lipoprotein-binding protein that is up-regulated by cholesterol loading of cells.
1992,
Pubmed Nielsen,
Estradiol and estrogen receptor-dependent stabilization of a minivitellogenin mRNA lacking 5,100 nucleotides of coding sequence.
1990,
Pubmed
,
Xenbase Pastori,
Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis.
1991,
Pubmed
,
Xenbase Pastori,
Estrogen-induced ribonuclease activity in Xenopus liver.
1991,
Pubmed
,
Xenbase Pastori,
The nuclease that selectively degrades albumin mRNA in vitro associates with Xenopus liver polysomes through the 80S ribosome complex.
1993,
Pubmed
,
Xenbase Prokipcak,
Purification and properties of a protein that binds to the C-terminal coding region of human c-myc mRNA.
1994,
Pubmed Schoenberg,
Characterization of mRNA endonucleases.
1999,
Pubmed van Dijk,
Identification of RNA sequences and structures involved in site-specific cleavage of IGF-II mRNAs.
1998,
Pubmed Wang,
Identification of an erythroid-enriched endoribonuclease activity involved in specific mRNA cleavage.
2000,
Pubmed Wu,
Establishment of stable cell lines expressing potentially toxic proteins by tetracycline-regulated and epitope-tagging methods.
1996,
Pubmed
