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Wnt growth factors regulate a variety of developmental processes by altering specific gene expression patterns. In vertebrates beta-catenin acts as transcriptional activator, which is needed to overcome target gene repression by Groucho/TLE proteins, and to permit promoter activation as the final consequence of Wnt signaling. However, the molecular mechanisms of transcriptional activation by beta-catenin are only poorly understood. Here we demonstrate that the closely related acetyltransferases p300 and CBP potentiate beta-catenin-mediated activation of the siamois promoter, a known Wnt target. beta-catenin and p300 also synergize to stimulate a synthetic reporter gene construct, whereas activation of the cyclin D1 promoter by beta-catenin is refractory to p300 stimulation. Axis formation and activation of the beta-catenin target genes siamois and Xnr-3 in Xenopus embryos are sensitive to the E1A oncoprotein, a known inhibitor of p300/CBP. The C-terminus of beta-catenin interacts directly with a region overlapping the CH-3 domain of p300. p300 could participate in alleviating promoter repression imposed by chromatin structure and in recruiting the basal transcription machinery to promoters of particular Wnt target genes.
Aberle,
Assembly of the cadherin-catenin complex in vitro with recombinant proteins.
1994, Pubmed
Aberle,
Assembly of the cadherin-catenin complex in vitro with recombinant proteins.
1994,
Pubmed Aberle,
beta-catenin is a target for the ubiquitin-proteasome pathway.
1997,
Pubmed Aberle,
Cadherin-catenin complex: protein interactions and their implications for cadherin function.
1996,
Pubmed Aoki,
Nuclear endpoint of Wnt signaling: neoplastic transformation induced by transactivating lymphoid-enhancing factor 1.
1999,
Pubmed Bannister,
CBP-induced stimulation of c-Fos activity is abrogated by E1A.
1995,
Pubmed Bauer,
Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein.
1998,
Pubmed Brannon,
XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus development.
1999,
Pubmed
,
Xenbase Brannon,
A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus.
1997,
Pubmed
,
Xenbase Cadigan,
Wnt signaling: a common theme in animal development.
1997,
Pubmed
,
Xenbase Cavallo,
Drosophila Tcf and Groucho interact to repress Wingless signalling activity.
1998,
Pubmed Chakravarti,
A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity.
1999,
Pubmed Chen,
A functional interaction between the histone deacetylase Rpd3 and the corepressor groucho in Drosophila development.
1999,
Pubmed Chen,
Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase.
1999,
Pubmed Eastman,
Regulation of LEF-1/TCF transcription factors by Wnt and other signals.
1999,
Pubmed Eckner,
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
1994,
Pubmed Edmondson,
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.
1996,
Pubmed Felzien,
Specificity of cyclin E-Cdk2, TFIIB, and E1A interactions with a common domain of the p300 coactivator.
1999,
Pubmed Fujii,
Molecular cloning and expression of Xenopus p300/CBP.
1998,
Pubmed
,
Xenbase Grant,
Histone acetyltransferase complexes.
1999,
Pubmed Hamamori,
Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A.
1999,
Pubmed Hecht,
Functional characterization of multiple transactivating elements in beta-catenin, some of which interact with the TATA-binding protein in vitro.
1999,
Pubmed Hsu,
Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.
1998,
Pubmed Huber,
Nuclear localization of beta-catenin by interaction with transcription factor LEF-1.
1996,
Pubmed
,
Xenbase Ishitani,
The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.
1999,
Pubmed
,
Xenbase Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase Kato,
Neuralization of the Xenopus embryo by inhibition of p300/ CREB-binding protein function.
1999,
Pubmed
,
Xenbase Kawasaki,
Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation.
1998,
Pubmed Korinek,
Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma.
1997,
Pubmed Korzus,
Transcription factor-specific requirements for coactivators and their acetyltransferase functions.
1998,
Pubmed Kwok,
Nuclear protein CBP is a coactivator for the transcription factor CREB.
1994,
Pubmed Levanon,
Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors.
1998,
Pubmed Martinez-Balbás,
The acetyltransferase activity of CBP stimulates transcription.
1998,
Pubmed Meneghini,
MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans.
1999,
Pubmed Miller,
Mechanism and function of signal transduction by the Wnt/beta-catenin and Wnt/Ca2+ pathways.
1999,
Pubmed Morin,
Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC.
1997,
Pubmed Munshi,
Acetylation of HMG I(Y) by CBP turns off IFN beta expression by disrupting the enhanceosome.
1998,
Pubmed Nakajima,
RNA helicase A mediates association of CBP with RNA polymerase II.
1997,
Pubmed Novak,
Cell adhesion and the integrin-linked kinase regulate the LEF-1 and beta-catenin signaling pathways.
1998,
Pubmed Palaparti,
The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3.
1997,
Pubmed Peifer,
A repeating amino acid motif shared by proteins with diverse cellular roles.
1994,
Pubmed Porfiri,
Induction of a beta-catenin-LEF-1 complex by wnt-1 and transforming mutants of beta-catenin.
1997,
Pubmed Prieve,
Nuclear localization and formation of beta-catenin-lymphoid enhancer factor 1 complexes are not sufficient for activation of gene expression.
1999,
Pubmed Puri,
Differential roles of p300 and PCAF acetyltransferases in muscle differentiation.
1997,
Pubmed Qiu,
An eukaryotic RuvB-like protein (RUVBL1) essential for growth.
1998,
Pubmed Rocheleau,
WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans.
1999,
Pubmed Roose,
The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
1998,
Pubmed
,
Xenbase Shiama,
The p300/CBP family: integrating signals with transcription factors and chromatin.
1997,
Pubmed Shtutman,
The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway.
1999,
Pubmed Sokol,
Wnt signaling and dorso-ventral axis specification in vertebrates.
1999,
Pubmed Tetsu,
Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells.
1999,
Pubmed Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase van de Wetering,
Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF.
1997,
Pubmed Vleminckx,
The C-terminal transactivation domain of beta-catenin is necessary and sufficient for signaling by the LEF-1/beta-catenin complex in Xenopus laevis.
1999,
Pubmed
,
Xenbase Waltzer,
Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling.
1998,
Pubmed Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed Yuan,
Human p300 protein is a coactivator for the transcription factor MyoD.
1996,
Pubmed
