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Absence of Wee1 ensures the meiotic cell cycle in Xenopus oocytes.
Nakajo N, Yoshitome S, Iwashita J, Iida M, Uto K, Ueno S, Okamoto K, Sagata N.
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Meiotic cells undergo two successive divisions without an intervening S phase. However, the mechanism of S-phase omission between the two meiotic divisions is largely unknown. Here we show that Wee1, a universal mitotic inhibitor, is absent in immature (but not mature) Xenopus oocytes, being down-regulated specifically during oogenesis; this down-regulation is most likely due to a translational repression. Even the modest ectopic expression of Wee1 in immature (meiosis I) oocytes can induce interphase nucleus reformation and DNA replication just after meiosis I. Thus, the presence of Wee1 during meiosis I converts the meiotic cell cycle into a mitotic-like cell cycle having S phase. In contrast, Myt1, a Wee1-related kinase, is present and directly involved in G(2) arrest of immature oocytes, but its ectopic expression has little effect on the meiotic cell cycle. These results strongly indicate that the absence of Wee1 in meiosis I ensures the meiotic cell cycle in Xenopus oocytes. Based on these results and the data published previously in other organisms, we suggest that absence of Wee1 may be a well-conserved mechanism for omitting interphase or S phase between the two meiotic divisions.
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Aligue,
Regulation of Schizosaccharomyces pombe Wee1 tyrosine kinase.
1997, Pubmed
Aligue,
Regulation of Schizosaccharomyces pombe Wee1 tyrosine kinase.
1997,
Pubmed Chau,
Mos-induced p42 mitogen-activated protein kinase activation stabilizes M-phase in Xenopus egg extracts after cyclin destruction.
1998,
Pubmed
,
Xenbase Coleman,
Cdc2 regulatory factors.
1994,
Pubmed Dasso,
Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus.
1990,
Pubmed
,
Xenbase Daya-Makin,
Regulation of p105wee1 and p34cdc2 during meiosis in Schizosaccharomyces pombe.
1992,
Pubmed de Moor,
Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.
1999,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Fattaey,
Myt1: a Wee1-type kinase that phosphorylates Cdc2 on residue Thr14.
1997,
Pubmed Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase Gabrielli,
A cdc2-related kinase oscillates in the cell cycle independently of cyclins G2/M and cdc2.
1992,
Pubmed
,
Xenbase Gard,
Microtubule organization during maturation of Xenopus oocytes: assembly and rotation of the meiotic spindles.
1992,
Pubmed
,
Xenbase Gautier,
Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation.
1991,
Pubmed
,
Xenbase Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase Grallert,
Dissociation of meiotic and mitotic roles of the fission yeast cdc2 gene.
1990,
Pubmed Grallert,
Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.
1991,
Pubmed Hartwell,
Checkpoints: controls that ensure the order of cell cycle events.
1989,
Pubmed Hecht,
DNA polymerases in mouse spermatogenic cells separated by sedimentation velocity.
1979,
Pubmed Hecht,
Changing DNA polymerase activities during the development of the testis in the mouse.
1976,
Pubmed Izumi,
Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
1992,
Pubmed
,
Xenbase Johnson,
Mammalian cell fusion: induction of premature chromosome condensation in interphase nuclei.
1970,
Pubmed Katsu,
Behavior of the components of maturation-promoting factor, cdc2 kinase and cyclin B, during oocyte maturation of goldfish.
1993,
Pubmed
,
Xenbase King,
Mitosis in transition.
1994,
Pubmed Kishimoto,
Cell cycle arrest and release in starfish oocytes and eggs.
1998,
Pubmed Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase Michael,
Coupling of mitosis to the completion of S phase through Cdc34-mediated degradation of Wee1.
1998,
Pubmed
,
Xenbase Millar,
The cdc25 M-phase inducer: an unconventional protein phosphatase.
1992,
Pubmed Mitra,
Regulation of the acquisition of meiotic competence in the mouse: changes in the subcellular localization of cdc2, cyclin B1, cdc25C and wee1, and in the concentration of these proteins and their transcripts.
1996,
Pubmed Morgan,
Principles of CDK regulation.
1995,
Pubmed Mueller,
Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15.
1995,
Pubmed
,
Xenbase Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase Murakami,
Mos positively regulates Xe-Wee1 to lengthen the first mitotic cell cycle of Xenopus.
1999,
Pubmed
,
Xenbase Murakami,
Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos.
1998,
Pubmed
,
Xenbase Nakajo,
Involvement of Chk1 kinase in prophase I arrest of Xenopus oocytes.
1999,
Pubmed
,
Xenbase Newport,
On the coupling between DNA replication and mitosis.
1989,
Pubmed
,
Xenbase Norbury,
Animal cell cycles and their control.
1992,
Pubmed Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed Nurse,
Ordering S phase and M phase in the cell cycle.
1994,
Pubmed Nurse,
Checkpoint pathways come of age.
1997,
Pubmed Ohsumi,
Meiosis-specific cell cycle regulation in maturing Xenopus oocytes.
1994,
Pubmed
,
Xenbase O'Keefe,
The c-mos gene product is required for cyclin B accumulation during meiosis of mouse eggs.
1991,
Pubmed Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase Picard,
Newly assembled cyclin B-cdc2 kinase is required to suppress DNA replication between meiosis I and meiosis II in starfish oocytes.
1996,
Pubmed Russell,
Checkpoints on the road to mitosis.
1998,
Pubmed Sagata,
Meiotic metaphase arrest in animal oocytes: its mechanisms and biological significance.
1996,
Pubmed Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase Schuetz,
Local control mechanisms during oogenesis and folliculogenesis.
1985,
Pubmed Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Sia,
Control of Swe1p degradation by the morphogenesis checkpoint.
1998,
Pubmed Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Strausfeld,
Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
1991,
Pubmed Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase Watanabe,
Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle.
1995,
Pubmed Yamashita,
Molecular mechanisms of meiotic maturation and arrest in fish and amphibian oocytes.
1998,
Pubmed
,
Xenbase Yamashita,
Molecular mechanisms of the activation of maturation-promoting factor during goldfish oocyte maturation.
1995,
Pubmed
,
Xenbase
