Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Coiled coils direct assembly of a cold-activated TRP channel.
Tsuruda PR, Julius D, Minor DL.
???displayArticle.abstract???
Transient receptor potential (TRP) channels mediate numerous sensory transduction processes and are thought to function as tetramers. TRP channel physiology is well studied; however, comparatively little is understood regarding TRP channel assembly. Here, we identify an autonomously folded assembly domain from the cold- and menthol-gated channel TRPM8. We show that the TRPM8 cytoplasmic C-terminal domain contains a coiled coil that is necessary for channel assembly and sufficient for tetramer formation. Cell biological experiments indicate that coiled-coil formation is required for proper channel maturation and trafficking and that the coiled-coil domain alone can act as a dominant-negative inhibitor of functional channel expression. Our data define an authentic TRP modular assembly domain, establish a clear role for coiled coils in ion channel assembly, demonstrate that coiled-coil assembly domains are a general feature of TRPM channels, and delineate a new tool that should be of general use in dissecting TRPM channel function.
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994, Pubmed,
Xenbase
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994,
Pubmed
,
Xenbase Becktel,
Protein stability curves.
1987,
Pubmed Bixby,
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels.
1999,
Pubmed Brauchi,
Clues to understanding cold sensation: thermodynamics and electrophysiological analysis of the cold receptor TRPM8.
2004,
Pubmed Chevesich,
Requirement for the PDZ domain protein, INAD, for localization of the TRP store-operated channel to a signaling complex.
1997,
Pubmed Clapham,
International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels.
2003,
Pubmed Clapham,
The TRP ion channel family.
2001,
Pubmed Clapham,
TRP channels as cellular sensors.
2003,
Pubmed Davare,
A beta2 adrenergic receptor signaling complex assembled with the Ca2+ channel Cav1.2.
2001,
Pubmed Dempski,
Oligosaccharyl transferase: gatekeeper to the secretory pathway.
2002,
Pubmed Deutsch,
Potassium channel ontogeny.
2002,
Pubmed Dunphy,
Compartmental organization of the Golgi stack.
1985,
Pubmed Edelhoch,
Spectroscopic determination of tryptophan and tyrosine in proteins.
1967,
Pubmed Engelke,
Structural domains required for channel function of the mouse transient receptor potential protein homologue TRP1beta.
2002,
Pubmed Erler,
Ca2+-selective transient receptor potential V channel architecture and function require a specific ankyrin repeat.
2004,
Pubmed García-Sanz,
Identification of a tetramerization domain in the C terminus of the vanilloid receptor.
2004,
Pubmed Gillo,
Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry.
1996,
Pubmed
,
Xenbase Harbury,
Crystal structure of an isoleucine-zipper trimer.
1994,
Pubmed Harbury,
A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants.
1993,
Pubmed Hellwig,
Homo- and heteromeric assembly of TRPV channel subunits.
2005,
Pubmed Hoenderop,
Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6.
2003,
Pubmed
,
Xenbase Howard,
Hypothesis: a helix of ankyrin repeats of the NOMPC-TRP ion channel is the gating spring of mechanoreceptors.
2004,
Pubmed Jenke,
C-terminal domains implicated in the functional surface expression of potassium channels.
2003,
Pubmed
,
Xenbase Julius,
Molecular mechanisms of nociception.
2001,
Pubmed Kanki,
A structural requirement for processing the cardiac K+ channel KCNQ1.
2004,
Pubmed Kedei,
Analysis of the native quaternary structure of vanilloid receptor 1.
2001,
Pubmed Kobertz,
K+ channels lacking the 'tetramerization' domain: implications for pore structure.
1999,
Pubmed
,
Xenbase Kreusch,
Crystal structure of the tetramerization domain of the Shaker potassium channel.
1998,
Pubmed Laue,
Sedimentation equilibrium as thermodynamic tool.
1995,
Pubmed Launay,
TRPM4 regulates calcium oscillations after T cell activation.
2004,
Pubmed Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed Li,
TRP and the PDZ protein, INAD, form the core complex required for retention of the signalplex in Drosophila photoreceptor cells.
2000,
Pubmed Liedtke,
Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor.
2000,
Pubmed Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed Lu,
T1-T1 interactions occur in ER membranes while nascent Kv peptides are still attached to ribosomes.
2001,
Pubmed
,
Xenbase Ludwig,
Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-à-go-go potassium channel.
1997,
Pubmed Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed Lupas,
The structure of alpha-helical coiled coils.
2005,
Pubmed Malashkevich,
The crystal structure of a five-stranded coiled coil in COMP: a prototype ion channel?
1996,
Pubmed Marx,
Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel.
2002,
Pubmed McKemy,
Identification of a cold receptor reveals a general role for TRP channels in thermosensation.
2002,
Pubmed
,
Xenbase Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase Montell,
The TRP channels, a remarkably functional family.
2002,
Pubmed Montell,
The TRP superfamily of cation channels.
2005,
Pubmed Nagaya,
Potassium channel alpha and beta subunits assemble in the endoplasmic reticulum.
1997,
Pubmed Peier,
A TRP channel that senses cold stimuli and menthol.
2002,
Pubmed Robinson,
Coupled tertiary folding and oligomerization of the T1 domain of Kv channels.
2005,
Pubmed
,
Xenbase Rohács,
PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain.
2005,
Pubmed Schaefer,
Homo- and heteromeric assembly of TRP channel subunits.
2005,
Pubmed Schmitt,
A recessive C-terminal Jervell and Lange-Nielsen mutation of the KCNQ1 channel impairs subunit assembly.
2000,
Pubmed
,
Xenbase Schulteis,
Subunit folding and assembly steps are interspersed during Shaker potassium channel biogenesis.
1998,
Pubmed
,
Xenbase Schwake,
A carboxy-terminal domain determines the subunit specificity of KCNQ K+ channel assembly.
2003,
Pubmed
,
Xenbase Shen,
Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins.
1995,
Pubmed Sotomayor,
In search of the hair-cell gating spring elastic properties of ankyrin and cadherin repeats.
2005,
Pubmed Strübing,
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
2001,
Pubmed Strübing,
Formation of novel TRPC channels by complex subunit interactions in embryonic brain.
2003,
Pubmed Thebault,
Novel role of cold/menthol-sensitive transient receptor potential melastatine family member 8 (TRPM8) in the activation of store-operated channels in LNCaP human prostate cancer epithelial cells.
2005,
Pubmed Thiyagarajan,
Activation-induced subcellular redistribution of G alpha(s) is dependent upon its unique N-terminus.
2002,
Pubmed
,
Xenbase Tsunoda,
The organization of INAD-signaling complexes by a multivalent PDZ domain protein in Drosophila photoreceptor cells ensures sensitivity and speed of signaling.
1999,
Pubmed Van Petegem,
Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
2004,
Pubmed Voets,
The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels.
2004,
Pubmed Woolfson,
The design of coiled-coil structures and assemblies.
2005,
Pubmed Xu,
TRPgamma, a drosophila TRP-related subunit, forms a regulated cation channel with TRPL.
2000,
Pubmed Zerangue,
An artificial tetramerization domain restores efficient assembly of functional Shaker channels lacking T1.
2000,
Pubmed
,
Xenbase Zhang,
Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells.
2004,
Pubmed Zhong,
The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry.
2002,
Pubmed Zhong,
Selective heteromeric assembly of cyclic nucleotide-gated channels.
2003,
Pubmed
