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Specificity and sensitivity of a human olfactory receptor functionally expressed in human embryonic kidney 293 cells and Xenopus Laevis oocytes.
Wetzel CH, Oles M, Wellerdieck C, Kuczkowiak M, Gisselmann G, Hatt H.
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Here, we provide the first evidence for functional expression of a human olfactory receptor protein (OR17-40) and show that recombinant olfactory receptors can be functionally expressed in heterologous systems. A mixture of 100 different odorants (Henkel 100) elicited a transient increase in intracellular [Ca(2+)] in human embryonic kidney 293 (HEK293) cells stably or transiently transfected with the plasmid pOR17-40. By subdividing the odorant mixture into progressively smaller groups, we identified a single component that represented the only effective substance: helional. Only the structurally closely related molecule heliotroplyacetone also activated the receptor. Other compounds, including piperonal, safrole, and vanillin, were completely ineffective. Mock-transfected cells and cells transfected with other receptors showed no change in intracellular [Ca(2+)] in response to odor stimulation. We were also able to functionally express OR17-40 in Xenopus laevis oocytes. Coexpression of a "reporter" channel allowed measurement of the response of oocytes injected with the cRNA of the human receptor to the odor mixture Henkel 100. The effective substances were the same (helional, heliotropylacetone) as those identified by functionally expressing the receptor in HEK293 cells and were active at the same, lower micromolar concentration. These findings open the possibility of now characterizing the sensitivity and specificity of many, if not all, of the hundreds of different human olfactory receptors.
Afshar,
Towards structural models of molecular recognition in olfactory receptors.
1998, Pubmed
Afshar,
Towards structural models of molecular recognition in olfactory receptors.
1998,
Pubmed Axel,
The molecular logic of smell.
1995,
Pubmed Ben-Arie,
Olfactory receptor gene cluster on human chromosome 17: possible duplication of an ancestral receptor repertoire.
1994,
Pubmed Buck,
A novel multigene family may encode odorant receptors: a molecular basis for odor recognition.
1991,
Pubmed Crowe,
Olfactory receptor-encoding genes and pseudogenes are expressed in humans.
1996,
Pubmed Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase Gadsby,
Regulation of CFTR channel gating.
1994,
Pubmed Glusman,
Sequence analysis in the olfactory receptor gene cluster on human chromosome 17: recombinatorial events affecting receptor diversity.
1996,
Pubmed Grygorczyk,
Detection of adenylate cyclase-coupled receptors in Xenopus oocytes by coexpression with cystic fibrosis transmembrane conductance regulator.
1995,
Pubmed
,
Xenbase Hansen,
Intercellular calcium signaling induced by extracellular adenosine 5'-triphosphate and mechanical stimulation in airway epithelial cells.
1993,
Pubmed Krautwurst,
Identification of ligands for olfactory receptors by functional expression of a receptor library.
1998,
Pubmed Lancet,
Olfactory receptors.
1993,
Pubmed Lancet,
Vertebrate olfactory reception.
1986,
Pubmed Lankiewicz,
Molecular cloning, functional expression, and pharmacological characterization of 5-hydroxytryptamine3 receptor cDNA and its splice variants from guinea pig.
1998,
Pubmed Mall,
cAMP stimulation of CFTR-expressing Xenopus oocytes activates a chromanol-inhibitable K+ conductance.
1996,
Pubmed
,
Xenbase Malnic,
Combinatorial receptor codes for odors.
1999,
Pubmed Mori,
Molecular recognition and olfactory processing in the mammalian olfactory system.
1995,
Pubmed Raming,
Cloning and expression of odorant receptors.
1993,
Pubmed Reed,
How does the nose know?
1990,
Pubmed Ressler,
A molecular dissection of spatial patterning in the olfactory system.
1994,
Pubmed Rouquier,
Distribution of olfactory receptor genes in the human genome.
1998,
Pubmed Sato,
Tuning specificities to aliphatic odorants in mouse olfactory receptor neurons and their local distribution.
1994,
Pubmed Selbie,
Novel G protein-coupled receptors: a gene family of putative human olfactory receptor sequences.
1992,
Pubmed Sengupta,
odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl.
1996,
Pubmed Shepherd,
Discrimination of molecular signals by the olfactory receptor neuron.
1994,
Pubmed Sicard,
Receptor cell responses to odorants: similarities and differences among odorants.
1984,
Pubmed Thüraüf,
Cyclic nucleotide-gated channels in identified human olfactory receptor neurons.
1996,
Pubmed Uezono,
Receptors that couple to 2 classes of G proteins increase cAMP and activate CFTR expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Wellerdieck,
Functional expression of odorant receptors of the zebrafish Danio rerio and of the nematode C. elegans in HEK293 cells.
1997,
Pubmed Wu,
Direct relationship between intracellular calcium mobilization and phospholipase D activation in prostaglandin E-stimulated human erythroleukemia cells.
1992,
Pubmed Zhang,
The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells.
1997,
Pubmed Zhao,
Functional expression of a mammalian odorant receptor.
1998,
Pubmed Zufall,
Rapid application and removal of second messengers to cyclic nucleotide-gated channels from olfactory epithelium.
1993,
Pubmed
