Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The effect of alpha2-delta and other accessory subunits on expression and properties of the calcium channel alpha1G.
Dolphin AC, Wyatt CN, Richards J, Beattie RE, Craig P, Lee JH, Cribbs LL, Volsen SG, Perez-Reyes E.
???displayArticle.abstract???
1. The effect has been examined of the accessory alpha2-delta and beta subunits on the properties of alpha1G currents expressed in monkey COS-7 cells and Xenopus oocytes. 2. In immunocytochemical experiments, the co-expression of alpha2-delta increased plasma membrane localization of expressed alpha1G and conversely, the heterologous expression of alpha1G increased immunostaining for endogenous alpha2-delta, suggesting an interaction between the two subunits. 3. Heterologous expression of alpha2-delta together with alpha1G in COS-7 cells increased the amplitude of expressed alpha1G currents by about 2-fold. This finding was confirmed in the Xenopus oocyte expression system. The truncated delta construct did not increase alpha1G current amplitude, or increase its plasma membrane expression. This indicates that it is the exofacial alpha2 domain that is involved in the enhancement by alpha2-delta. 4. Beta1b also produced an increase of functional expression of alpha1G, either in the absence or the presence of heterologously expressed alpha2-delta, whereas the other beta subunits had much smaller effects. 5. None of the accessory subunits had any marked influence on the voltage dependence or kinetics of the expressed alpha1G currents. These results therefore suggest that alpha2-delta and beta1b interact with alpha1G to increase trafficking of, or stabilize, functional alpha1G channels expressed at the plasma membrane.
Armstrong,
Two distinct populations of calcium channels in a clonal line of pituitary cells.
1985, Pubmed
Armstrong,
Two distinct populations of calcium channels in a clonal line of pituitary cells.
1985,
Pubmed Berrow,
Properties of cloned rat alpha1A calcium channels transiently expressed in the COS-7 cell line.
1997,
Pubmed Brice,
Importance of the different beta subunits in the membrane expression of the alpha1A and alpha2 calcium channel subunits: studies using a depolarization-sensitive alpha1A antibody.
1997,
Pubmed Brickley,
Use of site-directed antibodies to probe the topography of the alpha 2 subunit of voltage-gated Ca2+ channels.
1995,
Pubmed Campbell,
Voltage-dependent calcium channel beta-subunits in combination with alpha 1 subunits, have a GTPase activating effect to promote the hydrolysis of GTP by G alpha o in rat frontal cortex.
1995,
Pubmed Campbell,
Inhibition of the interaction of G protein G(o) with calcium channels by the calcium channel beta-subunit in rat neurones.
1995,
Pubmed Carbone,
A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones.
,
Pubmed Chien,
Roles of a membrane-localized beta subunit in the formation and targeting of functional L-type Ca2+ channels.
1995,
Pubmed Costantin,
Facilitation by the beta2a subunit of pore openings in cardiac Ca2+ channels.
1998,
Pubmed
,
Xenbase Craig,
Distribution of the voltage-dependent calcium channel alpha1G subunit mRNA and protein throughout the mature rat brain.
1999,
Pubmed Cribbs,
Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family.
1998,
Pubmed De Waard,
Subunit regulation of the neuronal alpha 1A Ca2+ channel expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase De Waard,
Identification of critical amino acids involved in alpha1-beta interaction in voltage-dependent Ca2+ channels.
1996,
Pubmed
,
Xenbase Felix,
Dissection of functional domains of the voltage-dependent Ca2+ channel alpha2delta subunit.
1997,
Pubmed
,
Xenbase Gao,
Complexes of the alpha1C and beta subunits generate the necessary signal for membrane targeting of class C L-type calcium channels.
1999,
Pubmed Gurnett,
Extracellular interaction of the voltage-dependent Ca2+ channel alpha2delta and alpha1 subunits.
1997,
Pubmed Gurnett,
Dual function of the voltage-dependent Ca2+ channel alpha 2 delta subunit in current stimulation and subunit interaction.
1996,
Pubmed
,
Xenbase Huguenard,
Low-threshold calcium currents in central nervous system neurons.
1996,
Pubmed Jeziorski,
Cloning and functional expression of a voltage-gated calcium channel alpha1 subunit from jellyfish.
1998,
Pubmed
,
Xenbase Jones,
Mechanism of auxiliary subunit modulation of neuronal alpha1E calcium channels.
1998,
Pubmed Klugbauer,
Molecular diversity of the calcium channel alpha2delta subunit.
1999,
Pubmed Kobrinsky,
Low- and high-voltage-activated calcium channel currents and their modulation in the dorsal root ganglion cell line ND7-23.
1994,
Pubmed Lacerda,
T-type and N-type calcium channels of Xenopus oocytes: evidence for specific interactions with beta subunits.
1994,
Pubmed
,
Xenbase Lambert,
Low-voltage-activated Ca2+ currents are generated by members of the CavT subunit family (alpha1G/H) in rat primary sensory neurons.
1998,
Pubmed Lambert,
T-type Ca2+ current properties are not modified by Ca2+ channel beta subunit depletion in nodosus ganglion neurons.
1997,
Pubmed Lee,
Cloning and expression of a novel member of the low voltage-activated T-type calcium channel family.
1999,
Pubmed
,
Xenbase Meir,
Known calcium channel alpha1 subunits can form low threshold small conductance channels with similarities to native T-type channels.
1998,
Pubmed Nowycky,
Three types of neuronal calcium channel with different calcium agonist sensitivity.
,
Pubmed Perez-Reyes,
Molecular characterization of a neuronal low-voltage-activated T-type calcium channel.
1998,
Pubmed
,
Xenbase Qin,
Modulation of human neuronal alpha 1E-type calcium channel by alpha 2 delta-subunit.
1998,
Pubmed
,
Xenbase Ren,
Functional expression and characterization of skeletal muscle dihydropyridine receptors in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Schreibmayer,
Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes.
1994,
Pubmed
,
Xenbase Shistik,
Ca2+ current enhancement by alpha 2/delta and beta subunits in Xenopus oocytes: contribution of changes in channel gating and alpha 1 protein level.
1995,
Pubmed
,
Xenbase Singer-Lahat,
Evidence for the existence of RNA of Ca(2+)-channel alpha 2/delta subunit in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Stephens,
Functional expression of rat brain cloned alpha1E calcium channels in COS-7 cells.
1997,
Pubmed Swick,
Promoter-cDNA-directed heterologous protein expression in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase Tareilus,
A Xenopus oocyte beta subunit: evidence for a role in the assembly/expression of voltage-gated calcium channels that is separate from its role as a regulatory subunit.
1997,
Pubmed
,
Xenbase Walker,
A beta 4 isoform-specific interaction site in the carboxyl-terminal region of the voltage-dependent Ca2+ channel alpha 1A subunit.
1998,
Pubmed Walker,
Subunit interaction sites in voltage-dependent Ca2+ channels: role in channel function.
1998,
Pubmed Wyatt,
The effect of overexpression of auxiliary Ca2+ channel subunits on native Ca2+ channel currents in undifferentiated mammalian NG108-15 cells.
1998,
Pubmed
