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Binding of 14-3-3 proteins near the nuclear localization sequence of Xenopus Cdc25 suppresses its ability to induce entry into mitosis. We have examined the intracellular localization of green fluorescent protein (GFP)-tagged wild-type Cdc25 or a mutant (S287A) that cannot bind 14-3-3 proteins. Upon coexpression with Myc-14-3-3epsilon, GFP-Cdc25-WT was predominantly cytoplasmic, whereas GFP-Cdc25-S287A was exclusively nuclear. Leptomycin B, an inhibitor of nuclear export, elicited a prompt redistribution of GFP-Cdc25-WT to the nucleus. Mutagenesis experiments demonstrated that Cdc25 contains multiple nuclear export sequences. These studies indicate that the binding of 14-3-3 proteins and nuclear export regulate the intracellular localization of Cdc25.
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10323858 ???displayArticle.pmcLink???PMC316939 ???displayArticle.link???Genes Dev ???displayArticle.grants???[+]
Blasina,
A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase.
1999, Pubmed
Blasina,
A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase.
1999,
Pubmed Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase Fukuda,
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.
1997,
Pubmed
,
Xenbase Furnari,
Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase.
1997,
Pubmed Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase Hagting,
MPF localization is controlled by nuclear export.
1998,
Pubmed Hoffmann,
Phosphorylation and activation of human cdc25-C by cdc2--cyclin B and its involvement in the self-amplification of MPF at mitosis.
1993,
Pubmed
,
Xenbase Izumi,
Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
1992,
Pubmed
,
Xenbase Izumi,
Phosphorylation and activation of the Xenopus Cdc25 phosphatase in the absence of Cdc2 and Cdk2 kinase activity.
1995,
Pubmed
,
Xenbase Jin,
Nuclear localization of cyclin B1 controls mitotic entry after DNA damage.
1998,
Pubmed Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Kumagai,
Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
1992,
Pubmed
,
Xenbase Kumagai,
14-3-3 proteins act as negative regulators of the mitotic inducer Cdc25 in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase Kumagai,
The Xenopus Chk1 protein kinase mediates a caffeine-sensitive pathway of checkpoint control in cell-free extracts.
1998,
Pubmed
,
Xenbase Li,
Nuclear localization of cyclin B1 mediates its biological activity and is regulated by phosphorylation.
1997,
Pubmed
,
Xenbase Liu,
Crystal structure of the zeta isoform of the 14-3-3 protein.
1995,
Pubmed
,
Xenbase Lopez-Girona,
Nuclear localization of Cdc25 is regulated by DNA damage and a 14-3-3 protein.
1999,
Pubmed Matsuoka,
Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
1998,
Pubmed Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed Moore,
Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1.
1999,
Pubmed
,
Xenbase Morgan,
Cyclin-dependent kinases: engines, clocks, and microprocessors.
1997,
Pubmed Ossareh-Nazari,
Evidence for a role of CRM1 in signal-mediated nuclear protein export.
1997,
Pubmed Peng,
Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.
1997,
Pubmed Sanchez,
Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.
1997,
Pubmed Thorson,
14-3-3 proteins are required for maintenance of Raf-1 phosphorylation and kinase activity.
1998,
Pubmed Toyoshima,
Nuclear export of cyclin B1 and its possible role in the DNA damage-induced G2 checkpoint.
1998,
Pubmed Wang,
Mutations in the hydrophobic surface of an amphipathic groove of 14-3-3zeta disrupt its interaction with Raf-1 kinase.
1998,
Pubmed Yaffe,
The structural basis for 14-3-3:phosphopeptide binding specificity.
1997,
Pubmed Yang,
Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1.
1998,
Pubmed
,
Xenbase Zhang,
The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells.
1997,
Pubmed
