Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2005 Oct 19;2420:3519-31. doi: 10.1038/sj.emboj.7600825.
Show Gene links
Show Anatomy links
Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates.
Franz C, Askjaer P, Antonin W, Iglesias CL, Haselmann U, Schelder M, de Marco A, Wilm M, Antony C, Mattaj IW.
???displayArticle.abstract???
Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995, Pubmed
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995,
Pubmed Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase Askjaer,
Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.
2002,
Pubmed Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001,
Pubmed Bodoor,
Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis.
1999,
Pubmed Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed Finlay,
A complex of nuclear pore proteins required for pore function.
1991,
Pubmed Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase Galy,
Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo.
2003,
Pubmed
,
Xenbase Gant,
Nuclear assembly.
1997,
Pubmed Geles,
A role for Caenorhabditis elegans importin IMA-2 in germ line and embryonic mitosis.
2002,
Pubmed Gigliotti,
Nup154, a new Drosophila gene essential for male and female gametogenesis is related to the nup155 vertebrate nucleoporin gene.
1998,
Pubmed Goldberg,
Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly.
1997,
Pubmed
,
Xenbase Grandi,
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
1997,
Pubmed
,
Xenbase Gruenbaum,
The nuclear lamina comes of age.
2005,
Pubmed Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Haraguchi,
Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes.
2000,
Pubmed Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase Hartl,
Nuclear assembly with lambda DNA in fractionated Xenopus egg extracts: an unexpected role for glycogen in formation of a higher order chromatin intermediate.
1994,
Pubmed
,
Xenbase Hawryluk-Gara,
Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex.
2005,
Pubmed
,
Xenbase Kenna,
Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex.
1996,
Pubmed Kerscher,
Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation.
2001,
Pubmed Kiger,
Developmental genetics of the essential Drosophila nucleoporin nup154: allelic differences due to an outward-directed promoter in the P-element 3' end.
1999,
Pubmed Liu,
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
2000,
Pubmed Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase Lutzmann,
Reconstitution of Nup157 and Nup145N into the Nup84 complex.
2005,
Pubmed Macaulay,
Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.
1996,
Pubmed
,
Xenbase Maduro,
Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system.
1995,
Pubmed Mattaj,
Sorting out the nuclear envelope from the endoplasmic reticulum.
2004,
Pubmed Maul,
Time sequence of nuclear pore formation in phytohemagglutinin-stimulated lymphocytes and in HeLa cells during the cell cycle.
1972,
Pubmed McDonald,
Cryomethods for thin section electron microscopy.
2002,
Pubmed Moore,
HCP-1, a protein involved in chromosome segregation, is localized to the centromere of mitotic chromosomes in Caenorhabditis elegans.
1999,
Pubmed Müller-Reichert,
Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure.
2003,
Pubmed Pellettieri,
Coordinate activation of maternal protein degradation during the egg-to-embryo transition in C. elegans.
2003,
Pubmed Poteryaev,
Involvement of the actin cytoskeleton and homotypic membrane fusion in ER dynamics in Caenorhabditis elegans.
2005,
Pubmed Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase Radu,
Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA.
1993,
Pubmed Rayala,
The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155.
2004,
Pubmed Rout,
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
2000,
Pubmed Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase Shulga,
Binding dynamics of structural nucleoporins govern nuclear pore complex permeability and may mediate channel gating.
2003,
Pubmed Strome,
Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans embryos.
2001,
Pubmed Suntharalingam,
Peering through the pore: nuclear pore complex structure, assembly, and function.
2003,
Pubmed Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Walther,
The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.
2002,
Pubmed
,
Xenbase Walther,
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.
2001,
Pubmed
,
Xenbase Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
,
Xenbase
