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Axin is encoded by the fused locus in mice and is required for normal vertebrate axis formation. It has recently been shown that axin associates with APC, beta-catenin and glycogen synthase kinase-3 (GSK-3) in a complex that appears to regulate the level of cytoplasmic beta-catenin. We have identified the Xenopus homologue of axin through its interaction with GSK-3b. Xenopus axin (Xaxin) is expressed maternally and throughout early development with a low level of ubiquitous expression. Xaxin also shows remarkably high expression in the anteriormesencephalon adjacent to the forebrain-midbrain boundary.
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10072781 ???displayArticle.link???Mech Dev ???displayArticle.grants???[+]
axin1 (axin1) gene expression in Xenopus laevis embryos, NF stage 28, as assayed by in situ hybridization, lateral view, anteriorleft, dorsal up.
Fig. 2. Expression pattern of Xaxin. (A) Whole mount in situ hybridization in Stage II oocytes. (B) Sixty-four cell and blastula stage embryos. (C) Gastrula
stage embryo. (D) Tailbud stage, lateral view (during late neurula, a band is first observed in the neural plate. This staining becomes more intense and
localizes to the anteriormidbrain, adjacent to the forebrainidbrain boundary by the tailbud stage). (E) Dorsal view of tailbudembryo. Scale bar, 0.5 mm, in
each figure. (F) Double in situ hybridization with Xaxin (black arrow) and engrailed (open arrow) in stage 32 embryo. (G) Double in-situ hybridization with
Xaxin (black arrow) and Xwnt-2B (open arrow). (H) Whole oocytes and embryos were subjected to RT-PCR analysis to determine the temporal expression
pattern of Axin. c-Src is used as a loading control (lower panel). Stages are indicated at the top. A negative control without reverse transcriptase is indicated
in the right lane (-RT).
