Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The Mos protein kinase is a key regulator of vertebrate oocyte maturation. Oocyte-specific Mos protein expression is subject to translational control. In the frog Xenopus, the translation of Mos protein requires the progesterone-induced polyadenylation of the maternal Mos mRNA, which is present in the oocytecytoplasm. Both the Xenopus p42 mitogen-activated protein kinase (MAPK) and maturation-promoting factor (MPF) signaling pathways have been proposed to mediate progesterone-stimulated oocyte maturation. In this study, we have determined the relative contributions of the MAPK and MPF signaling pathways to Mos mRNA polyadenylation. We report that progesterone-induced Mos mRNA polyadenylation was attenuated in oocytes expressing the MAPK phosphatase rVH6. Moreover, inhibition of MAPK signaling blocked progesterone-induced Mos protein accumulation. Activation of the MAPK pathway by injection of RNA encoding Mos was sufficient to induce both the polyadenylation of synthetic Mos mRNA substrates and the accumulation of endogenous Mos protein in the absence of MPF signaling. Activation of MPF, by injection of cyclin B1 RNA or purified cyclin B1 protein, also induced both Mos protein accumulation and Mos mRNA polyadenylation. However, this action of MPF required MAPK activity. By contrast, the cytoplasmic polyadenylation of maternal cyclin B1 mRNA was stimulated by MPF in a MAPK-independent manner, thus revealing a differential regulation of maternal mRNA polyadenylation by the MAPK and MPF signaling pathways. We propose that MAPK-stimulated Mos mRNA cytoplasmic polyadenylation is a key component of the positive-feedback loop, which contributes to the all-or-none process of oocyte maturation.
Abrieu,
Mitogen-activated protein kinase activation down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in G2-arrested oocytes.
1997, Pubmed,
Xenbase
Abrieu,
Mitogen-activated protein kinase activation down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in G2-arrested oocytes.
1997,
Pubmed
,
Xenbase Alessi,
PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo.
1995,
Pubmed Ballantyne,
Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development.
1995,
Pubmed
,
Xenbase Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase Barkoff,
Meiotic maturation in Xenopus requires polyadenylation of multiple mRNAs.
1998,
Pubmed
,
Xenbase Bienroth,
Purification of the cleavage and polyadenylation factor involved in the 3'-processing of messenger RNA precursors.
1991,
Pubmed Blenis,
Signal transduction via the MAP kinases: proceed at your own RSK.
1993,
Pubmed Brondello,
Constitutive MAP kinase phosphatase (MKP-1) expression blocks G1 specific gene transcription and S-phase entry in fibroblasts.
1995,
Pubmed Colgan,
Cell-cycle related regulation of poly(A) polymerase by phosphorylation.
1996,
Pubmed
,
Xenbase Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase Cross,
PD 98059 prevents establishment of the spindle assembly checkpoint and inhibits the G2-M transition in meiotic but not mitotic cell cycles in Xenopus.
1998,
Pubmed
,
Xenbase Daar,
A characterization of cytostatic factor activity from Xenopus eggs and c-mos-transformed cells.
1991,
Pubmed
,
Xenbase Davis,
Transcriptional regulation by MAP kinases.
1995,
Pubmed de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase Ferrell,
The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes.
1998,
Pubmed
,
Xenbase Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Freeman,
Meiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.
1991,
Pubmed
,
Xenbase Freeman,
Xenopus homolog of the mos protooncogene transforms mammalian fibroblasts and induces maturation of Xenopus oocytes.
1989,
Pubmed
,
Xenbase Gardner,
Measuring activation of kinases in mitogen-activated protein kinase regulatory network.
1994,
Pubmed Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed Gebauer,
Cloning and characterization of a Xenopus poly(A) polymerase.
1995,
Pubmed
,
Xenbase Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase Haccard,
Induction of Xenopus oocyte meiotic maturation by MAP kinase.
1995,
Pubmed
,
Xenbase Hake,
Translational regulation of maternal mRNA.
1997,
Pubmed
,
Xenbase Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase Huang,
Dependence of Mos-induced Cdc2 activation on MAP kinase function in a cell-free system.
1996,
Pubmed
,
Xenbase Kanki,
Progression from meiosis I to meiosis II in Xenopus oocytes requires de novo translation of the mosxe protooncogene.
1991,
Pubmed
,
Xenbase Keller,
Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA.
1991,
Pubmed Kuge,
Cytoplasmic 3' poly(A) addition induces 5' cap ribose methylation: implications for translational control of maternal mRNA.
1995,
Pubmed
,
Xenbase Kumagai,
Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors.
1995,
Pubmed
,
Xenbase Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Macdonald,
Translational regulation of maternal mRNAs.
1996,
Pubmed MacNicol,
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF.
1993,
Pubmed
,
Xenbase MacNicol,
pXen, a utility vector for the expression of GST-fusion proteins in Xenopus laevis oocytes and embryos.
1997,
Pubmed
,
Xenbase MacNicol,
Regulation of Raf-1-dependent signaling during early Xenopus development.
1995,
Pubmed
,
Xenbase Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed
,
Xenbase Matten,
Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.
1996,
Pubmed
,
Xenbase McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Mercer,
An analysis of the rate of metallothionein mRNA poly(A)-shortening using RNA blot hybridization.
1985,
Pubmed Mourey,
A novel cytoplasmic dual specificity protein tyrosine phosphatase implicated in muscle and neuronal differentiation.
1996,
Pubmed Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase Murthy,
The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.
1995,
Pubmed Murthy,
Characterization of the multisubunit cleavage-polyadenylation specificity factor from calf thymus.
1992,
Pubmed Muslin,
Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
1993,
Pubmed
,
Xenbase Nebreda,
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
1995,
Pubmed
,
Xenbase Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase Pause,
Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function.
1994,
Pubmed Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Qian,
Activated polo-like kinase Plx1 is required at multiple points during mitosis in Xenopus laevis.
1998,
Pubmed
,
Xenbase Roy,
Mos proto-oncogene function during oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Shibuya,
Activation of the Xenopus oocyte mitogen-activated protein kinase pathway by Mos is independent of Raf.
1996,
Pubmed
,
Xenbase Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed Stebbins-Boaz,
Multiple sequence elements and a maternal mRNA product control cdk2 RNA polyadenylation and translation during early Xenopus development.
1994,
Pubmed
,
Xenbase Stebbins-Boaz,
Translational control during early development.
1997,
Pubmed Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Sun,
MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.
1993,
Pubmed Thuresson,
Multiple forms of poly(A) polymerases in human cells.
1994,
Pubmed VanRenterghem,
Reconstitution of p21ras-dependent and -independent mitogen-activated protein kinase activation in a cell-free system.
1993,
Pubmed
,
Xenbase Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase
