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A polysomal ribonuclease involved in the destabilization of albumin mRNA is a novel member of the peroxidase gene family.
Chernokalskaya E, Dubell AN, Cunningham KS, Hanson MN, Dompenciel RE, Schoenberg DR.
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We have purified an approximately 60 kDa endoribonuclease from Xenopus liver polysomes with properties expected for a messenger RNase involved in the estrogen-regulated destabilization of serum protein mRNAs (Dompenciel et al., 1995, J Biol Chem 270:6108-6118). The present report describes the cloning of this protein and its identification as a novel member of the peroxidase gene family. This novel enzyme, named polysomal RNase 1, or PMR-1 has 57% sequence identity with myeloperoxidase, and like that protein, appears to be processed from a larger precursor. Unlike myeloperoxidase, however, PMR-1 lacks N-linked oligosaccharide, heme, and peroxidase activity. Western blot and immunoprecipitation experiments using epitope-specific antibodies to the derived protein sequence confirm the identity of the cloned cDNA to the protein originally isolated from polysomes. The 80 kDa pre-PMR-1 expressed in a recombinant baculovirus was not processed to the 60 kDa form in Sf9 cells and lacks RNase activity. However, the baculovirus-expressed mature 60-kDa form of the enzyme has RNase activity. The recombinant protein is an endonuclease that shows selectivity for albumin versus ferritin mRNA. While it does not cleave at consensus APyrUGA elements, recombinant PMR-1 generates the same minor cleavage products from albumin mRNA as PMR-1 purified from liver. Finally, we show estrogen induces only a small increase in the amount of PMR-1. This result is consistent with earlier data suggesting estrogen activates mRNA decay through a posttranslational pathway.
Andersson,
Spectral analysis of lactoperoxidase. Evidence for a common heme in mammalian peroxidases.
1996, Pubmed
Andersson,
Spectral analysis of lactoperoxidase. Evidence for a common heme in mammalian peroxidases.
1996,
Pubmed Aronica,
Estrogen action via the cAMP signaling pathway: stimulation of adenylate cyclase and cAMP-regulated gene transcription.
1994,
Pubmed Aström,
In vitro deadenylation of mammalian mRNA by a HeLa cell 3' exonuclease.
1991,
Pubmed Aström,
Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.
1992,
Pubmed Bashkirov,
A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates.
1997,
Pubmed Binder,
Estrogen-induced destabilization and associated degradation intermediates of apolipoprotein II mRNA.
1990,
Pubmed Binder,
Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening.
1994,
Pubmed Brock,
Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation.
1983,
Pubmed
,
Xenbase Brown,
Endonucleolytic cleavage of a maternal homeo box mRNA in Xenopus oocytes.
1990,
Pubmed
,
Xenbase Brown,
Sequence-specific endonucleolytic cleavage and protection of mRNA in Xenopus and Drosophila.
1993,
Pubmed
,
Xenbase Caruccio,
Purification of a human polyribosome-associated 3' to 5' exoribonuclease.
1994,
Pubmed Chernokalskaya,
Cleavage properties of an estrogen-regulated polysomal ribonuclease involved in the destabilization of albumin mRNA.
1997,
Pubmed
,
Xenbase Christiansen,
A guanosine quadruplex and two stable hairpins flank a major cleavage site in insulin-like growth factor II mRNA.
1994,
Pubmed Couttet,
Messenger RNA deadenylylation precedes decapping in mammalian cells.
1997,
Pubmed Dompenciel,
Purification and characterization of an estrogen-regulated Xenopus liver polysomal nuclease involved in the selective destabilization of albumin mRNA.
1995,
Pubmed
,
Xenbase Fenna,
Structure of the green heme in myeloperoxidase.
1995,
Pubmed Gray,
Iron regulatory protein prevents binding of the 43S translation pre-initiation complex to ferritin and eALAS mRNAs.
1994,
Pubmed Hirling,
Mutational analysis of the [4Fe-4S]-cluster converting iron regulatory factor from its RNA-binding form to cytoplasmic aconitase.
1994,
Pubmed Körner,
Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease.
1997,
Pubmed Migliaccio,
Tyrosine kinase/p21ras/MAP-kinase pathway activation by estradiol-receptor complex in MCF-7 cells.
1996,
Pubmed Moskaitis,
The estrogen-regulated destabilization of Xenopus albumin mRNA is independent of translation.
1991,
Pubmed
,
Xenbase Nagy,
Glyceraldehyde-3-phosphate dehydrogenase selectively binds AU-rich RNA in the NAD(+)-binding region (Rossmann fold).
1995,
Pubmed Nakagawa,
AUH, a gene encoding an AU-specific RNA binding protein with intrinsic enoyl-CoA hydratase activity.
1995,
Pubmed Pastori,
The nuclease that selectively degrades albumin mRNA in vitro associates with Xenopus liver polysomes through the 80S ribosome complex.
1993,
Pubmed
,
Xenbase Pastori,
Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis.
1991,
Pubmed
,
Xenbase Pastori,
Estrogen-induced ribonuclease activity in Xenopus liver.
1991,
Pubmed
,
Xenbase Peitsch,
ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling.
1996,
Pubmed Preiss,
The mRNA-binding protein COLBP is glutamate dehydrogenase.
1995,
Pubmed Prokipcak,
Purification and properties of a protein that binds to the C-terminal coding region of human c-myc mRNA.
1994,
Pubmed Riegel,
Posttranscriptional regulation of albumin gene expression in Xenopus liver: evidence for an estrogen receptor-dependent mechanism.
1987,
Pubmed
,
Xenbase Ross,
mRNA stability in mammalian cells.
1995,
Pubmed Ross,
H4 histone messenger RNA decay in cell-free extracts initiates at or near the 3' terminus and proceeds 3' to 5'.
1986,
Pubmed Scheper,
The cis-acting elements involved in endonucleolytic cleavage of the 3' UTR of human IGF-II mRNAs bind a 50 kDa protein.
1996,
Pubmed Schoenberg,
Extranuclear estrogen-regulated destabilization of Xenopus laevis serum albumin mRNA.
1989,
Pubmed
,
Xenbase Shapiro,
Estrogen regulation of gene transcription and mRNA stability.
1989,
Pubmed Stoeckle,
Removal of a 3' non-coding sequence is an initial step in degradation of gro alpha mRNA and is regulated by interleukin-1.
1992,
Pubmed Taylor,
Unique autolytic cleavage of human myeloperoxidase. Implications for the involvement of active site MET409.
1992,
Pubmed Vreken,
The rate-limiting step in yeast PGK1 mRNA degradation is an endonucleolytic cleavage in the 3'-terminal part of the coding region.
1992,
Pubmed
