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Proc Natl Acad Sci U S A
1998 Nov 24;9524:14184-9. doi: 10.1073/pnas.95.24.14184.
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Unmasking the functions of the chromaffin cell alpha7 nicotinic receptor by using short pulses of acetylcholine and selective blockers.
López MG, Montiel C, Herrero CJ, García-Palomero E, Mayorgas I, Hernández-Guijo JM, Villarroya M, Olivares R, Gandía L, McIntosh JM, Olivera BM, García AG.
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Methyllycaconitine (MLA), alpha-conotoxin ImI, and alpha-bungarotoxin inhibited the release of catecholamines triggered by brief pulses of acetylcholine (ACh) (100 microM, 5 s) applied to fast-superfused bovine adrenal chromaffin cells, with IC50s of 100 nM for MLA and 300 nM for alpha-conotoxin ImI and alpha-bungarotoxin. MLA (100 nM), alpha-conotoxin ImI (1 microM), and alpha-bungarotoxin (1 microM) halved the entry of 45Ca2+ stimulated by 5-s pulses of 300 microM ACh applied to incubated cells. These supramaximal concentrations of alpha7 nicotinic receptor blockers depressed by 30% (MLA), 25% (alpha-bungarotoxin), and 50% (alpha-conotoxin ImI) the inward current generated by 1-s pulses of 100 microM ACh, applied to voltage-clamped chromaffin cells. In Xenopus oocytes expressing rat brain alpha7 neuronal nicotinic receptor for acetylcholine nAChR, the current generated by 1-s pulses of ACh was blocked by MLA, alpha-conotoxin ImI, and alpha-bungarotoxin with IC50s of 0.1 nM, 100 nM, and 1.6 nM, respectively; the current through alpha3 beta4 nAChR was unaffected by alpha-conotoxin ImI and alpha-bungarotoxin, and weakly blocked by MLA (IC50 = 1 microM). The functions of controlling the electrical activity, the entry of Ca2+, and the ensuing exocytotic response of chromaffin cells were until now exclusively attributed to alpha3 beta4 nAChR; the present results constitute the first evidence to support a prominent role of alpha7 nAChR in controlling such functions, specially under the more physiological conditions used here to stimulate chromaffin cells with brief pulses of ACh.
Afar,
Nicotine-induced intracellular calcium changes are not antagonized by alpha-bungarotoxin in adrenal medullary cells.
1994, Pubmed
Afar,
Nicotine-induced intracellular calcium changes are not antagonized by alpha-bungarotoxin in adrenal medullary cells.
1994,
Pubmed Borges,
Continuous monitoring of catecholamine release from perfused cat adrenals.
1986,
Pubmed Brown,
Department of Pharmacology, The School of Pharmacy, University of London, London, Great Britain.
1977,
Pubmed Carbonetto,
Nonequivalence of alpha-bungarotoxin receptors and acetylcholine receptors in chick sympathetic neurons.
1978,
Pubmed Chan,
A role for the nicotinic alpha-bungarotoxin receptor in neurite outgrowth in PC12 cells.
1993,
Pubmed Changeux,
Use of a snake venom toxin to characterize the cholinergic receptor protein.
1970,
Pubmed Codignola,
alpha-Conotoxin imperialis I inhibits nicotine-evoked hormone release and cell proliferation in human neuroendocrine carcinoma cells.
1996,
Pubmed Criado,
Primary structure of an agonist binding subunit of the nicotinic acetylcholine receptor from bovine adrenal chromaffin cells.
1992,
Pubmed Criado,
Differential expression of alpha-bungarotoxin-sensitive neuronal nicotinic receptors in adrenergic chromaffin cells: a role for transcription factor Egr-1.
1997,
Pubmed DOUGLAS,
On the mode of action of acetylcholine in evoking adrenal medullary secretion: increased uptake of calcium during the secretory response.
1962,
Pubmed Duggan,
Alpha-bungarotoxin, cobra neurotoxin and excitation of Renshaw cells by acetylcholine.
1976,
Pubmed Fenwick,
Sodium and calcium channels in bovine chromaffin cells.
1982,
Pubmed Freedman,
Alpha-bungarotoxin binding to hippocampal interneurons: immunocytochemical characterization and effects on growth factor expression.
1993,
Pubmed García-Guzmán,
alpha-Bungarotoxin-sensitive nicotinic receptors on bovine chromaffin cells: molecular cloning, functional expression and alternative splicing of the alpha 7 subunit.
1995,
Pubmed
,
Xenbase Groot-Kormelink,
A reporter mutation approach shows incorporation of the "orphan" subunit beta3 into a functional nicotinic receptor.
1998,
Pubmed
,
Xenbase Gu,
Characterization and localization of adrenal nicotinic acetylcholine receptors: evidence that mAb35-nicotinic receptors are the principal receptors mediating adrenal catecholamine secretion.
1996,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Hory-Lee,
The nicotinic blocking agents d-tubocurare and alpha-bungarotoxin save motoneurons from naturally occurring death in the absence of neuromuscular blockade.
1995,
Pubmed Johnson,
alpha-Conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: preferential inhibition of homomeric alpha 7 and alpha 9 receptors.
1995,
Pubmed
,
Xenbase Kageyama,
Effect of alpha-bungarotoxin and etorphine on acetylcholine-evoked release of endogenous and radiolabeled catecholamines from primary culture of adrenal chromaffin cells.
1984,
Pubmed Kilpatrick,
Ion channels and membrane potential in stimulus-secretion coupling in adrenal medulla cells.
1981,
Pubmed Kouvelas,
Chick sympathetic neurons develop receptors for alpha-bungarotoxin in vitro, but the toxin does not block nicotinic receptors.
1978,
Pubmed Kumakura,
Modulation of nicotinic receptors by opiate receptor agonists in cultured adrenal chromaffin cells.
1980,
Pubmed McGehee,
Nicotine enhancement of fast excitatory synaptic transmission in CNS by presynaptic receptors.
1995,
Pubmed McIntosh,
A nicotinic acetylcholine receptor ligand of unique specificity, alpha-conotoxin ImI.
1994,
Pubmed Montiel,
Serotonergic effects of dotarizine in coronary artery and in oocytes expressing 5-HT2 receptors.
1997,
Pubmed
,
Xenbase Moro,
Separation and culture of living adrenaline- and noradrenaline-containing cells from bovine adrenal medullae.
1990,
Pubmed Myers,
alpha-Conotoxins, small peptide probes of nicotinic acetylcholine receptors.
1991,
Pubmed Palma,
Neuronal nicotinic alpha 7 receptor expressed in Xenopus oocytes presents five putative binding sites for methyllycaconitine.
1996,
Pubmed
,
Xenbase Patrick,
alpha-Bungarotoxin binding and cholinergic receptor function on a rat sympathetic nerve line.
1977,
Pubmed Pugh,
Neuronal acetylcholine receptors that bind alpha-bungarotoxin mediate neurite retraction in a calcium-dependent manner.
1994,
Pubmed Quik,
The alpha-bungarotoxin site and its relation to the cholinergic and nerve growth factor mediated increases in tyrosine hydroxylase activity in cultures of sympathetic ganglia and chromaffin cells.
1982,
Pubmed Quik,
alpha-Bungarotoxin blocks the nicotinic receptor mediated increase in cell number in a neuroendocrine cell line.
1994,
Pubmed Quik,
Marked up-regulation of the beta-bungarotoxin site in adrenal chromaffin cells by specific nicotinic antagonists.
1987,
Pubmed Revah,
Mutations in the channel domain alter desensitization of a neuronal nicotinic receptor.
1991,
Pubmed
,
Xenbase Trifaró,
Morphological characteristics and stimulus-secretion coupling in bovine adcrenal chromaffin cell cultures.
1980,
Pubmed Ullian,
Rapid synaptic transmission in the avian ciliary ganglion is mediated by two distinct classes of nicotinic receptors.
1997,
Pubmed Vijayaraghavan,
Nicotinic receptors that bind alpha-bungarotoxin on neurons raise intracellular free Ca2+.
1992,
Pubmed Ward,
Methyllycaconitine: a selective probe for neuronal alpha-bungarotoxin binding sites.
1990,
Pubmed Wilson,
The acetylcholine receptor of the adrenal medulla.
1977,
Pubmed Zhang,
Neuronal acetylcholine receptors that bind alpha-bungarotoxin with high affinity function as ligand-gated ion channels.
1994,
Pubmed
,
Xenbase Zhang,
Synaptic currents generated by neuronal acetylcholine receptors sensitive to alpha-bungarotoxin.
1996,
Pubmed
