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Macroscopic and microscopic properties of a cloned glutamate transporter/chloride channel.
Wadiche JI, Kavanaugh MP.
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The behavior of a Cl- channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of approximately 5A. Glutamate flux exhibited a much greater temperature dependence than Cl- flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl- conductance and a maximum channel Po < 1. The glutamate binding rate was similar to estimates for receptor binding. After glutamate binding, channels activated rapidly followed by a relaxation phase. Differences in the macroscopic kinetics of channels activated by concentration jumps of L-glutamate or D-aspartate were correlated with differences in uptake kinetics, indicating a close correspondence of channel gating to state transitions in the transporter cycle.
Anderson,
Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction.
1973, Pubmed
Anderson,
Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction.
1973,
Pubmed Arriza,
Cloning and expression of a human neutral amino acid transporter with structural similarity to the glutamate transporter gene family.
1993,
Pubmed
,
Xenbase Arriza,
Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance.
1997,
Pubmed
,
Xenbase Asztely,
Extrasynaptic glutamate spillover in the hippocampus: dependence on temperature and the role of active glutamate uptake.
1997,
Pubmed Barbour,
Electrogenic glutamate uptake in glial cells is activated by intracellular potassium.
1988,
Pubmed Bergles,
Glutamate transporter currents in bergmann glial cells follow the time course of extrasynaptic glutamate.
1997,
Pubmed Bergles,
Synaptic activation of glutamate transporters in hippocampal astrocytes.
1997,
Pubmed Billups,
Anion conductance behavior of the glutamate uptake carrier in salamander retinal glial cells.
1996,
Pubmed Borlinghaus,
Fast charge translocations associated with partial reactions of the Na,K-pump: I. Current and voltage transients after photochemical release of ATP.
1987,
Pubmed Cammack,
Channel behavior in a gamma-aminobutyrate transporter.
1996,
Pubmed Clements,
The time course of glutamate in the synaptic cleft.
1992,
Pubmed Diamond,
Transporters buffer synaptically released glutamate on a submillisecond time scale.
1997,
Pubmed Eliasof,
Excitatory amino acid transporters of the salamander retina: identification, localization, and function.
1998,
Pubmed
,
Xenbase Eliasof,
Retinal glial cell glutamate transporter is coupled to an anionic conductance.
1996,
Pubmed Erecińska,
Aspartate transport in synaptosomes from rat brain.
1983,
Pubmed Fairman,
An excitatory amino-acid transporter with properties of a ligand-gated chloride channel.
1995,
Pubmed
,
Xenbase Galli,
Norepinephrine transporters have channel modes of conduction.
1996,
Pubmed Grant,
A glutamate-activated chloride current in cone-driven ON bipolar cells of the white perch retina.
1995,
Pubmed Grunewald,
Conformational changes monitored on the glutamate transporter GLT-1 indicate the existence of two neurotransmitter-bound states.
1995,
Pubmed Halm,
Anion permeation in an apical membrane chloride channel of a secretory epithelial cell.
1992,
Pubmed HODGKIN,
Measurement of current-voltage relations in the membrane of the giant axon of Loligo.
1952,
Pubmed Jonas,
Quantal components of unitary EPSCs at the mossy fibre synapse on CA3 pyramidal cells of rat hippocampus.
1993,
Pubmed Kanner,
Active transport of L-glutamate by membrane vesicles isolated from rat brain.
1978,
Pubmed Kavanaugh,
Mutation of an amino acid residue influencing potassium coupling in the glutamate transporter GLT-1 induces obligate exchange.
1997,
Pubmed Kavanaugh,
Voltage dependence of facilitated arginine flux mediated by the system y+ basic amino acid transporter.
1993,
Pubmed
,
Xenbase Klamo,
Kinetics and stoichiometry of a proton/myo-inositol cotransporter.
1996,
Pubmed
,
Xenbase Larsson,
Noise analysis of the glutamate-activated current in photoreceptors.
1996,
Pubmed Lehre,
Differential expression of two glial glutamate transporters in the rat brain: quantitative and immunocytochemical observations.
1995,
Pubmed Lin,
Single-channel currents produced by the serotonin transporter and analysis of a mutation affecting ion permeation.
1996,
Pubmed
,
Xenbase Maconochie,
A method for making solution changes in the sub-millisecond range at the tip of a patch pipette.
1989,
Pubmed Mager,
Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Malandro,
Molecular biology of mammalian amino acid transporters.
1996,
Pubmed
,
Xenbase Nelson,
Hydrogen ion cotransport by the renal brush border glutamate transporter.
1983,
Pubmed Otis,
Postsynaptic glutamate transport at the climbing fiber-Purkinje cell synapse.
1997,
Pubmed
,
Xenbase Otis,
Anion currents and predicted glutamate flux through a neuronal glutamate transporter.
1998,
Pubmed Picaud,
Glutamate-gated chloride channel with glutamate-transporter-like properties in cone photoreceptors of the tiger salamander.
1995,
Pubmed Picaud,
Cone photoreceptors respond to their own glutamate release in the tiger salamander.
1995,
Pubmed Rothstein,
Localization of neuronal and glial glutamate transporters.
1994,
Pubmed Sarantis,
A presynaptic action of glutamate at the cone output synapse.
1988,
Pubmed Scanziani,
Use-dependent increases in glutamate concentration activate presynaptic metabotropic glutamate receptors.
1997,
Pubmed Sigworth,
The variance of sodium current fluctuations at the node of Ranvier.
1980,
Pubmed Stallcup,
Coupled transport of glutamate and sodium in a cerebellar nerve cell line.
1979,
Pubmed Szatkowski,
Non-vesicular release of glutamate from glial cells by reversed electrogenic glutamate uptake.
1990,
Pubmed Tachibana,
L-glutamate-induced depolarization in solitary photoreceptors: a process that may contribute to the interaction between photoreceptors in situ.
1988,
Pubmed Wadiche,
Ion fluxes associated with excitatory amino acid transport.
1995,
Pubmed
,
Xenbase Wadiche,
Kinetics of a human glutamate transporter.
1995,
Pubmed
,
Xenbase Yamada,
EAAT4 is a post-synaptic glutamate transporter at Purkinje cell synapses.
1996,
Pubmed Zampighi,
A method for determining the unitary functional capacity of cloned channels and transporters expressed in Xenopus laevis oocytes.
1995,
Pubmed
,
Xenbase Zerangue,
ASCT-1 is a neutral amino acid exchanger with chloride channel activity.
1996,
Pubmed
,
Xenbase Zerangue,
Flux coupling in a neuronal glutamate transporter.
1996,
Pubmed
,
Xenbase
