Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
2006 Feb 01;4515:646-56. doi: 10.1007/s00424-005-1498-z.
Show Gene links
Show Anatomy links
Molecular cloning and functional characterization of the Aplysia FMRFamide-gated Na+ channel.
Furukawa Y, Miyawaki Y, Abe G.
???displayArticle.abstract???
FMRFamide-gated Na+ channel (FaNaC) is the only known peptide-gated ion channel, which belongs to the epithelial Na+ channel/degenerin (ENaC/DEG) family. We have cloned a putative FaNaC from the Aplysia kurodai CNS library using PCR, and examined its characteristics in Xenopus oocytes. A. kurodai FaNaC (AkFaNaC) comprised with 653 amino acids, and the sequence predicts two putative membrane domains and a large extracellular domain as in other members of the ENaC/DEG family. In oocytes expressing AkFaNaC, FMRFamide evoked amiloride-sensitive Na+ current. Different from the known FaNaCs (Helix and Helisoma FaNaCs), AkFaNaC was blocked by external Ca2+ but not by Mg2+. Also, desensitization of the current was enhanced by Mg2+ but not by Ca2+. The FMRFamide-gated current was depressed in both low and high pH. These results indicate that AkFaNaC is an FaNaC of Aplysia, and that the channel has Aplysia specific functional domains.
Adams,
Paradoxical stimulation of a DEG/ENaC channel by amiloride.
1999, Pubmed,
Xenbase
Adams,
Paradoxical stimulation of a DEG/ENaC channel by amiloride.
1999,
Pubmed
,
Xenbase Belkin,
FMRFamide produces biphasic modulation of the LFS motor neurons in the neural circuit of the siphon withdrawal reflex of Aplysia by activating Na+ and K+ currents.
1993,
Pubmed Benos,
Functional domains within the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels.
1999,
Pubmed Coscoy,
The pre-transmembrane 1 domain of acid-sensing ion channels participates in the ion pore.
1999,
Pubmed Coscoy,
The Phe-Met-Arg-Phe-amide-activated sodium channel is a tetramer.
1998,
Pubmed
,
Xenbase Cottrell,
The first peptide-gated ion channel.
1997,
Pubmed
,
Xenbase Cottrell,
Multiple actions of a molluscan cardioexcitatory neuropeptide and related peptides on identified Helix neurones.
1984,
Pubmed Cottrell,
Location of a ligand recognition site of FMRFamide-gated Na(+) channels.
2001,
Pubmed
,
Xenbase Cottrell,
The neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) can activate a ligand-gated ion channel in Helix neurones.
1990,
Pubmed Cropper,
FRF peptides in the ARC neuromuscular system of Aplysia: purification and physiological actions.
1994,
Pubmed Ebberink,
The brain of Lymnaea contains a family of FMRFamide-like peptides.
1987,
Pubmed Fujisawa,
The FMRFamide-related decapeptide of Mytilus contains a D-amino acid residue.
1992,
Pubmed Green,
Activity modes and modulation of the peptide-gated Na(+) channel of Helix neurones.
2002,
Pubmed Green,
The neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) directly gates two ion channels in an identified Helix neurone.
1994,
Pubmed Immke,
Protons open acid-sensing ion channels by catalyzing relief of Ca2+ blockade.
2003,
Pubmed Ishikawa,
Regulation of the epithelial Na+ channel by cytosolic ATP.
2003,
Pubmed Ismailov,
Identification of an amiloride binding domain within the alpha-subunit of the epithelial Na+ channel.
1997,
Pubmed
,
Xenbase Jeziorski,
Cloning and expression of a FMRFamide-gated Na(+) channel from Helisoma trivolvis and comparison with the native neuronal channel.
2000,
Pubmed
,
Xenbase Kellenberger,
Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure.
2002,
Pubmed Kellenberger,
On the molecular basis of ion permeation in the epithelial Na+ channel.
1999,
Pubmed
,
Xenbase Lingueglia,
Cloning of the amiloride-sensitive FMRFamide peptide-gated sodium channel.
1995,
Pubmed
,
Xenbase McNicholas,
Diversity of channels generated by different combinations of epithelial sodium channel subunits.
1997,
Pubmed
,
Xenbase Palmer,
Voltage-dependent block by amiloride and other monovalent cations of apical Na channels in the toad urinary bladder.
1984,
Pubmed Paukert,
Identification of the Ca2+ blocking site of acid-sensing ion channel (ASIC) 1: implications for channel gating.
2004,
Pubmed
,
Xenbase Perry,
Neuronal expression of an FMRFamide-gated Na+ channel and its modulation by acid pH.
2001,
Pubmed Pfaffinger,
Cloning and expression of an Aplysia K+ channel and comparison with native Aplysia K+ currents.
1991,
Pubmed
,
Xenbase Poët,
Exploration of the pore structure of a peptide-gated Na+ channel.
2001,
Pubmed
,
Xenbase Price,
Seven FMRFamide-related and two SCP-related cardioactive peptides from Helix.
1990,
Pubmed Rice,
EMBOSS: the European Molecular Biology Open Software Suite.
2000,
Pubmed Ruben,
Analysis of FMRF-amide effects on Aplysia bursting neurons.
1986,
Pubmed Schild,
Identification of amino acid residues in the alpha, beta, and gamma subunits of the epithelial sodium channel (ENaC) involved in amiloride block and ion permeation.
1997,
Pubmed
,
Xenbase Segal,
Influence of voltage and extracellular Na(+) on amiloride block and transport kinetics of rat epithelial Na(+) channel expressed in Xenopus oocytes.
2002,
Pubmed
,
Xenbase Sheng,
Epithelial sodium channel pore region. structure and role in gating.
2001,
Pubmed
,
Xenbase Shimizu,
Cumulative inactivation and the pore domain in the Kv1 channels.
2002,
Pubmed
,
Xenbase Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed Waldmann,
Functional degenerin-containing chimeras identify residues essential for amiloride-sensitive Na+ channel function.
1995,
Pubmed Weber,
Ion currents of Xenopus laevis oocytes: state of the art.
1999,
Pubmed
,
Xenbase Woodhull,
Ionic blockage of sodium channels in nerve.
1973,
Pubmed Zhainazarov,
Single-channel currents of a peptide-gated sodium channel expressed in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
