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The initiation of anaphase and exit from mitosis depend on the anaphase-promoting complex (APC), which mediates the ubiquitin-dependent proteolysis of anaphase-inhibiting proteins and mitotic cyclins. We have analyzed whether protein phosphatases are required for mitotic APC activation. In Xenopus egg extracts APC activation occurs normally in the presence of protein phosphatase 1 inhibitors, suggesting that the anaphase defects caused by protein phosphatase 1 mutation in several organisms are not due to a failure to activate the APC. Contrary to this, the initiation of mitotic cyclin B proteolysis is prevented by inhibitors of protein phosphatase 2A such as okadaic acid. Okadaic acid induces an activity that inhibits cyclin B ubiquitination. We refer to this activity as inhibitor of mitotic proteolysis because it also prevents the degradation of other APC substrates. A similar activity exists in extracts of Xenopus eggs that are arrested at the second meiotic metaphase by the cytostatic factor activity of the protein kinase mos. In Xenopus eggs, the initiation of anaphase II may therefore be prevented by an inhibitor of APC-dependent ubiquitination.
Abrieu,
MAP kinase does not inactivate, but rather prevents the cyclin degradation pathway from being turned on in Xenopus egg extracts.
1996, Pubmed,
Xenbase
Abrieu,
MAP kinase does not inactivate, but rather prevents the cyclin degradation pathway from being turned on in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Amon,
Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle.
1994,
Pubmed Aristarkhov,
E2-C, a cyclin-selective ubiquitin carrier protein required for the destruction of mitotic cyclins.
1996,
Pubmed Axton,
One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis.
1990,
Pubmed Brandeis,
The proteolysis of mitotic cyclins in mammalian cells persists from the end of mitosis until the onset of S phase.
1996,
Pubmed Cano,
Neither ERK nor JNK/SAPK MAP kinase subtypes are essential for histone H3/HMG-14 phosphorylation or c-fos and c-jun induction.
1995,
Pubmed Chen,
Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores.
1996,
Pubmed
,
Xenbase Cohen,
The structure and regulation of protein phosphatases.
1989,
Pubmed Cohen-Fix,
Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
1996,
Pubmed
,
Xenbase Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase Coux,
Structure and functions of the 20S and 26S proteasomes.
1996,
Pubmed Doonan,
The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1.
1989,
Pubmed Dudley,
A synthetic inhibitor of the mitogen-activated protein kinase cascade.
1995,
Pubmed Félix,
Cdc2 H1 kinase is negatively regulated by a type 2A phosphatase in the Xenopus early embryonic cell cycle: evidence from the effects of okadaic acid.
1990,
Pubmed
,
Xenbase Fernandez,
Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit.
1992,
Pubmed Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase Funabiki,
Cut2 proteolysis required for sister-chromatid seperation in fission yeast.
1996,
Pubmed Ghosh,
Okadaic acid inhibits sister chromatid separation in mammalian cells.
1992,
Pubmed Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Gorbsky,
Cell cycle checkpoints: arresting progress in mitosis.
1997,
Pubmed
,
Xenbase Gotoh,
Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
1990,
Pubmed Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase Hershko,
Components of a system that ligates cyclin to ubiquitin and their regulation by the protein kinase cdc2.
1994,
Pubmed Hisamoto,
The Glc7 type 1 protein phosphatase of Saccharomyces cerevisiae is required for cell cycle progression in G2/M.
1994,
Pubmed Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase Hunt,
The requirements for protein synthesis and degradation, and the control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo.
1992,
Pubmed Irniger,
Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast.
1995,
Pubmed Ishii,
Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+.
1996,
Pubmed Jessus,
Tyrosine phosphorylation of p34cdc2 and p42 during meiotic maturation of Xenopus oocyte. Antagonistic action of okadaic acid and 6-DMAP.
1991,
Pubmed
,
Xenbase Jones,
Activation of the Xenopus cyclin degradation machinery by full-length cyclin A.
1996,
Pubmed
,
Xenbase Kameshita,
A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel.
1989,
Pubmed King,
Mitosis in transition.
1994,
Pubmed King,
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.
1995,
Pubmed
,
Xenbase Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase Kosako,
Mitogen-activated protein kinase kinase is required for the mos-induced metaphase arrest.
1994,
Pubmed
,
Xenbase Kumagai,
Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
1992,
Pubmed
,
Xenbase Kung,
Cell line-specific differences in the control of cell cycle progression in the absence of mitosis.
1990,
Pubmed Lahav-Baratz,
Reversible phosphorylation controls the activity of cyclosome-associated cyclin-ubiquitin ligase.
1995,
Pubmed Li,
MAD2 associates with the cyclosome/anaphase-promoting complex and inhibits its activity.
1997,
Pubmed
,
Xenbase Li,
Feedback control of mitosis in budding yeast.
1991,
Pubmed Li,
Identification of a human mitotic checkpoint gene: hsMAD2.
1996,
Pubmed Lorca,
An okadaic acid-sensitive phosphatase negatively controls the cyclin degradation pathway in amphibian eggs.
1991,
Pubmed
,
Xenbase Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Moriguchi,
Purification and identification of a major activator for p38 from osmotically shocked cells. Activation of mitogen-activated protein kinase kinase 6 by osmotic shock, tumor necrosis factor-alpha, and H2O2.
1996,
Pubmed Murray,
Cell cycle extracts.
1991,
Pubmed Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase Nigg,
Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation.
1996,
Pubmed
,
Xenbase Ohkura,
Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis.
1988,
Pubmed Ohkura,
The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
1989,
Pubmed O'Keefe,
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
1989,
Pubmed Parker,
Radiolabeling of proteins.
1990,
Pubmed Peters,
Identification of BIME as a subunit of the anaphase-promoting complex.
1996,
Pubmed
,
Xenbase Peters,
Proteasomes: protein degradation machines of the cell.
1994,
Pubmed Picard,
Involvement of protein phosphatases 1 and 2A in the control of M phase-promoting factor activity in starfish.
1989,
Pubmed Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Rock,
Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules.
1994,
Pubmed Sagata,
Meiotic metaphase arrest in animal oocytes: its mechanisms and biological significance.
1996,
Pubmed Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Schwab,
Yeast Hct1 is a regulator of Clb2 cyclin proteolysis.
1997,
Pubmed Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
,
Xenbase Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase Sigrist,
Drosophila fizzy-related down-regulates mitotic cyclins and is required for cell proliferation arrest and entry into endocycles.
1997,
Pubmed
,
Xenbase Sorger,
Coupling cell division and cell death to microtubule dynamics.
1997,
Pubmed Sudakin,
The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.
1995,
Pubmed Takenaka,
MAP kinase is required for the spindle assembly checkpoint but is dispensable for the normal M phase entry and exit in Xenopus egg cell cycle extracts.
1997,
Pubmed
,
Xenbase Tournebize,
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
1997,
Pubmed
,
Xenbase Vandré,
Inhibition of mitosis by okadaic acid: possible involvement of a protein phosphatase 2A in the transition from metaphase to anaphase.
1992,
Pubmed Visintin,
CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis.
1997,
Pubmed Walker,
Multiple roles for protein phosphatase 1 in regulating the Xenopus early embryonic cell cycle.
1992,
Pubmed
,
Xenbase Wang,
A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells.
1997,
Pubmed
,
Xenbase Whitfield,
The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition.
1990,
Pubmed Yu,
Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation.
1996,
Pubmed
,
Xenbase Zachariae,
Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae.
1996,
Pubmed
