Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3' UTR cis-acting element (termed VLE), which also mediates sequence-specific binding of several proteins. One protein, the 69-kD Vg1 RBP, associates Vg1 RNA to microtubules in vitro. Here we show that Vg1 RBP-binding sites correlate with vegetal localization. Purification and cloning of Vg1 RBP revealed five RNA-binding motifs: four KH and one RRM domains. Surprisingly, Vg1 RBP is highly homologous to the zipcode binding protein implicated in the microfilament-mediated localization of beta actin mRNA in fibroblasts. These data support Vg1 RBP's direct role in vegetal localization and suggest the existence of a general, evolutionarily conserved mechanism for mRNA targeting.
???displayArticle.pubmedLink???
9620847 ???displayArticle.pmcLink???PMC316865 ???displayArticle.link???Genes Dev
Bassell,
Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.
1998, Pubmed
Bassell,
Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.
1998,
Pubmed Deshler,
Localization of Xenopus Vg1 mRNA by Vera protein and the endoplasmic reticulum.
1997,
Pubmed
,
Xenbase Elisha,
Vg1 RNA binding protein mediates the association of Vg1 RNA with microtubules in Xenopus oocytes.
1995,
Pubmed
,
Xenbase Erdélyi,
Requirement for Drosophila cytoplasmic tropomyosin in oskar mRNA localization.
1995,
Pubmed Forristall,
Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2.
1995,
Pubmed
,
Xenbase Gautreau,
Two copies of a subelement from the Vg1 RNA localization sequence are sufficient to direct vegetal localization in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Gavis,
Expeditions to the pole: RNA localization in Xenopus and Drosophila.
1997,
Pubmed
,
Xenbase Kaminski,
Direct evidence that polypyrimidine tract binding protein (PTB) is essential for internal initiation of translation of encephalomyocarditis virus RNA.
1995,
Pubmed Kislauskis,
Isoform-specific 3'-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments.
1993,
Pubmed Kislauskis,
Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.
1994,
Pubmed Kloc,
Translocation of repetitive RNA sequences with the germ plasm in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Kloc,
Two distinct pathways for the localization of RNAs at the vegetal cortex in Xenopus oocytes.
1995,
Pubmed
,
Xenbase Kuznetsov,
Actin-dependent organelle movement in squid axoplasm.
1992,
Pubmed Lillie,
Suppression of a myosin defect by a kinesin-related gene.
1992,
Pubmed Lustig,
Expression cloning of a Xenopus T-related gene (Xombi) involved in mesodermal patterning and blastopore lip formation.
1996,
Pubmed
,
Xenbase Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase Mosquera,
A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain.
1993,
Pubmed
,
Xenbase Mowry,
Complex formation between stage-specific oocyte factors and a Xenopus mRNA localization element.
1996,
Pubmed
,
Xenbase Mowry,
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Müeller-Pillasch,
Cloning of a gene highly overexpressed in cancer coding for a novel KH-domain containing protein.
1997,
Pubmed Ross,
Characterization of a beta-actin mRNA zipcode-binding protein.
1997,
Pubmed Schwartz,
A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs.
1992,
Pubmed
,
Xenbase Siomi,
Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome.
1994,
Pubmed Siomi,
RNA-binding proteins as regulators of gene expression.
1997,
Pubmed Stennard,
The Xenopus T-box gene, Antipodean, encodes a vegetally localised maternal mRNA and can trigger mesoderm formation.
1996,
Pubmed
,
Xenbase St Johnston,
The intracellular localization of messenger RNAs.
1995,
Pubmed Sundell,
Requirement of microfilaments in sorting of actin messenger RNA.
1991,
Pubmed Woehlke,
Microtubule interaction site of the kinesin motor.
1997,
Pubmed Yisraeli,
The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.
1988,
Pubmed
,
Xenbase Yisraeli,
A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA.
1990,
Pubmed
,
Xenbase Zhang,
Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning.
1996,
Pubmed
,
Xenbase Zhou,
RNA transport to the vegetal cortex of Xenopus oocytes.
1996,
Pubmed
,
Xenbase Zhou,
Localization of Xcat-2 RNA, a putative germ plasm component, to the mitochondrial cloud in Xenopus stage I oocytes.
1996,
Pubmed
,
Xenbase
