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Estrogen response elements function as allosteric modulators of estrogen receptor conformation.
Wood JR, Greene GL, Nardulli AM.
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The estrogen receptor (ER) is a ligand-dependent transcription factor that regulates the expression of estrogen-responsive genes. ER-mediated transcriptional changes are brought about by interaction of the ER with the estrogen response element (ERE). In this study, we examined the interaction of the Xenopus laevis ER DNA binding domain (DBD) and the intact ER with the X. laevis vitellogenin A2 ERE and the human pS2 ERE. Using gel mobility shift, DNase I footprinting, and methylation interference assays, we demonstrated that the DBD bound only as a dimer to the A2 ERE. However, the DBD bound as a monomer to the consensus pS2 ERE half site at lower DBD concentrations and then as a homodimer to the consensus and imperfect pS2 ERE half site at higher DBD concentrations. Antibody supershift experiments carried out with partially purified, yeast-expressed full-length ER demonstrated that three ER-specific antibodies interacted differentially with A2 and pS2 ERE-bound ER, indicating that receptor epitopes were differentially exposed. Furthermore, partial digestion of the A2 and pS2 ERE-bound ER with chymotrypsin or trypsin produced distinct protease cleavage patterns. Taken together, these data provide evidence that differential interaction of the DBD with the A2 and pS2 EREs brings about global changes in ER conformation. The conformational changes in ER induced by individual ERE sequences could lead to association of the receptor with different transcription factors and assist in the differential modulation of estrogen-responsive genes in target cells.
Alber,
Protein-DNA interactions: how GCN4 binds DNA.
1993, Pubmed
Alber,
Protein-DNA interactions: how GCN4 binds DNA.
1993,
Pubmed Beekman,
Transcriptional activation by the estrogen receptor requires a conformational change in the ligand binding domain.
1993,
Pubmed Berry,
Role of the two activating domains of the oestrogen receptor in the cell-type and promoter-context dependent agonistic activity of the anti-oestrogen 4-hydroxytamoxifen.
1990,
Pubmed Blaustein,
Estrogen receptor immunoreactivity in rat brain: rapid effects of estradiol injection.
1993,
Pubmed Bourguet,
Crystal structure of the ligand-binding domain of the human nuclear receptor RXR-alpha.
1995,
Pubmed Chang,
The role of estrogen response elements in expression of the Xenopus laevis vitellogenin B1 gene.
1992,
Pubmed
,
Xenbase Fawell,
Inhibition of estrogen receptor-DNA binding by the "pure" antiestrogen ICI 164,384 appears to be mediated by impaired receptor dimerization.
1990,
Pubmed Fawell,
Characterization and colocalization of steroid binding and dimerization activities in the mouse estrogen receptor.
1990,
Pubmed Frankel,
Modular structure of transcription factors: implications for gene regulation.
1991,
Pubmed Fujita,
Independent modes of transcriptional activation by the p50 and p65 subunits of NF-kappa B.
1992,
Pubmed Furlow,
High affinity binding of the estrogen receptor to a DNA response element does not require homodimer formation or estrogen.
1993,
Pubmed Green,
Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor.
,
Pubmed
,
Xenbase Greene,
Immunochemical studies of estrogen receptors.
1984,
Pubmed Hansen,
Conformational transitions of the estrogen receptor monomer. Effects of estrogens, antiestrogen, and temperature.
1986,
Pubmed Härd,
Solution structure of the glucocorticoid receptor DNA-binding domain.
1990,
Pubmed Klein-Hitpass,
A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor.
1988,
Pubmed
,
Xenbase Klein-Hitpass,
Specific binding of estrogen receptor to the estrogen response element.
1989,
Pubmed
,
Xenbase Kuiper,
Cloning of a novel receptor expressed in rat prostate and ovary.
1996,
Pubmed Lefstin,
Influence of a steroid receptor DNA-binding domain on transcriptional regulatory functions.
1994,
Pubmed Littlewood,
A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins.
1995,
Pubmed Luisi,
Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA.
1991,
Pubmed Martinez,
Cooperative binding of estrogen receptor to imperfect estrogen-responsive DNA elements correlates with their synergistic hormone-dependent enhancer activity.
1989,
Pubmed
,
Xenbase Metzger,
Characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells.
1995,
Pubmed Montano,
Human estrogen receptor ligand activity inversion mutants: receptors that interpret antiestrogens as estrogens and estrogens as antiestrogens and discriminate among different antiestrogens.
1996,
Pubmed Nardulli,
Binding of the estrogen receptor DNA-binding domain to the estrogen response element induces DNA bending.
1992,
Pubmed
,
Xenbase Nardulli,
Human estrogen receptor bound to an estrogen response element bends DNA.
1993,
Pubmed Nardulli,
Estrogen receptor-induced DNA bending: orientation of the bend and replacement of an estrogen response element with an intrinsic DNA bending sequence.
1995,
Pubmed Nardulli,
Purified estrogen receptor DNA binding domain expressed in Escherichia coli activates transcription of an estrogen-responsive promoter in cultured cells.
1991,
Pubmed
,
Xenbase Nardulli,
Estrogen receptor affinity and location of consensus and imperfect estrogen response elements influence transcription activation of simplified promoters.
1996,
Pubmed
,
Xenbase Nunez,
The 5' flanking region of the pS2 gene contains a complex enhancer region responsive to oestrogens, epidermal growth factor, a tumour promoter (TPA), the c-Ha-ras oncoprotein and the c-jun protein.
1989,
Pubmed Pabo,
Transcription factors: structural families and principles of DNA recognition.
1992,
Pubmed Ponglikitmongkol,
Genomic organization of the human oestrogen receptor gene.
1988,
Pubmed Potthoff,
Effects of wild type and mutant estrogen receptors on DNA flexibility, DNA bending, and transcription activation.
1996,
Pubmed Renaud,
Crystal structure of the RAR-gamma ligand-binding domain bound to all-trans retinoic acid.
1995,
Pubmed Schreiber,
Identification of a novel lymphoid specific octamer binding protein (OTF-2B) by proteolytic clipping bandshift assay (PCBA).
1988,
Pubmed Schwabe,
The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements.
1993,
Pubmed Schwabe,
DNA recognition by the oestrogen receptor: from solution to the crystal.
1993,
Pubmed Schwabe,
The oestrogen receptor recognizes an imperfectly palindromic response element through an alternative side-chain conformation.
1995,
Pubmed Spolar,
Coupling of local folding to site-specific binding of proteins to DNA.
1994,
Pubmed Staal,
Distinct conformations of vitamin D receptor/retinoid X receptor-alpha heterodimers are specified by dinucleotide differences in the vitamin D-responsive elements of the osteocalcin and osteopontin genes.
1996,
Pubmed Starr,
Intracellular receptors use a common mechanism to interpret signaling information at response elements.
1996,
Pubmed Steitz,
Structural studies of protein-nucleic acid interaction: the sources of sequence-specific binding.
1990,
Pubmed Talanian,
Sequence-specific DNA binding by a short peptide dimer.
1990,
Pubmed Tan,
DNA binding-induced conformational change of the yeast transcriptional activator PRTF.
1990,
Pubmed Tasset,
Distinct classes of transcriptional activating domains function by different mechanisms.
1990,
Pubmed Tora,
The human estrogen receptor has two independent nonacidic transcriptional activation functions.
1989,
Pubmed Tzukerman,
Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions.
1994,
Pubmed von Hippel,
Protein-DNA recognition: new perspectives and underlying themes.
1994,
Pubmed Wagner,
A structural role for hormone in the thyroid hormone receptor.
1995,
Pubmed Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase Webster,
The hormone-binding domains of the estrogen and glucocorticoid receptors contain an inducible transcription activation function.
1988,
Pubmed
