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We have added constitutively active MAP kinase/ERK kinase (MEK), an activator of the mitogen-activated protein kinase (MAPK) signaling pathway, to cycling Xenopus egg extracts at various times during the cell cycle. p42MAPK activation during entry into M-phase arrested the cell cycle in metaphase, as has been shown previously. Unexpectedly, p42MAPK activation during interphase inhibited entry into M-phase. In these interphase-arrested extracts, H1 kinase activity remained low, Cdc2 was tyrosine phosphorylated, and nuclei continued to enlarge. The interphase arrest was overcome by recombinant cyclin B. In other experiments, p42MAPK activation by MEK or by Mos inhibited Cdc2 activation by cyclin B. PD098059, a specific inhibitor of MEK, blocked the effects of MEK(QP) and Mos. Mos-induced activation of p42MAPK did not inhibit DNA replication. These results indicate that, in addition to the established role of p42MAPK activation in M-phase arrest, the inappropriate activation of p42MAPK during interphase prevents normal entry into M-phase.
Alessi,
PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo.
1995, Pubmed
Alessi,
PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo.
1995,
Pubmed Barth,
Role of cdc25-C phosphatase in the immediate G2 delay induced by the exogenous factors epidermal growth factor and phorbolester.
1996,
Pubmed Bottorff,
RAS signalling is abnormal in a c-raf1 MEK1 double mutant.
1995,
Pubmed Boulton,
Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.
1991,
Pubmed Cobb,
How MAP kinases are regulated.
1995,
Pubmed Cobb,
Extracellular signal-regulated kinases: ERKs in progress.
1991,
Pubmed Coleman,
Cdc2 regulatory factors.
1994,
Pubmed Daar,
The ras oncoprotein and M-phase activity.
1991,
Pubmed
,
Xenbase Davis,
Mitosis-specific monoclonal antibodies block cleavage in amphibian embryos.
1989,
Pubmed
,
Xenbase Druker,
Oncogenes, growth factors, and signal transduction.
1989,
Pubmed Erikson,
In vivo phosphorylation and activation of ribosomal protein S6 kinases during Xenopus oocyte maturation.
1989,
Pubmed
,
Xenbase Erikson,
Structure, expression, and regulation of protein kinases involved in the phosphorylation of ribosomal protein S6.
1991,
Pubmed
,
Xenbase Fang,
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.
1991,
Pubmed
,
Xenbase Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase Haccard,
Induction of Xenopus oocyte meiotic maturation by MAP kinase.
1995,
Pubmed
,
Xenbase Hattori,
Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system.
1992,
Pubmed
,
Xenbase Hoffmann,
Phosphorylation and activation of human cdc25-C by cdc2--cyclin B and its involvement in the self-amplification of MPF at mitosis.
1993,
Pubmed
,
Xenbase Izumi,
Phosphorylation of Xenopus cyclins B1 and B2 is not required for cell cycle transitions.
1991,
Pubmed
,
Xenbase Izumi,
Elimination of cdc2 phosphorylation sites in the cdc25 phosphatase blocks initiation of M-phase.
1993,
Pubmed
,
Xenbase Izumi,
Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
1992,
Pubmed
,
Xenbase Kuang,
cdc25 is one of the MPM-2 antigens involved in the activation of maturation-promoting factor.
1994,
Pubmed
,
Xenbase Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Kumagai,
Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
1992,
Pubmed
,
Xenbase Kumagai,
The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
1991,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Laird,
Raf-1 is activated during mitosis.
1995,
Pubmed Lange-Carter,
Assay of MEK kinases.
1995,
Pubmed Lange-Carter,
A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.
1993,
Pubmed Lovrić,
Activation of Mil/Raf protein kinases in mitotic cells.
1996,
Pubmed Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase O'Connell,
Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation.
1997,
Pubmed Peng,
Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.
1997,
Pubmed Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Robbins,
Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro.
1993,
Pubmed Ruderman,
MAP kinase and the activation of quiescent cells.
1993,
Pubmed Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Sagata,
Meiotic metaphase arrest in animal oocytes: its mechanisms and biological significance.
1996,
Pubmed Sanchez,
Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.
1997,
Pubmed Shibuya,
Activation of p42 MAP kinase and the release of oocytes from cell cycle arrest.
1992,
Pubmed Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
,
Xenbase Shibuya,
Activation of the Xenopus oocyte mitogen-activated protein kinase pathway by Mos is independent of Raf.
1996,
Pubmed
,
Xenbase Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase Shibuya,
Stabilization and enhancement of primary cytostatic factor (CSF) by ATP and NaF in amphibian egg cytosols.
1988,
Pubmed
,
Xenbase Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed Sun,
MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.
1993,
Pubmed Tachibana,
MAP kinase links the fertilization signal transduction pathway to the G1/S-phase transition in starfish eggs.
1997,
Pubmed Takenaka,
MAP kinase is required for the spindle assembly checkpoint but is dispensable for the normal M phase entry and exit in Xenopus egg cell cycle extracts.
1997,
Pubmed
,
Xenbase van der Velden,
Mitotic arrest caused by the amino terminus of Xenopus cyclin B2.
1993,
Pubmed
,
Xenbase van der Velden,
Cell cycle-regulated degradation of Xenopus cyclin B2 requires binding to p34cdc2.
1994,
Pubmed
,
Xenbase Vigers,
A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs.
1991,
Pubmed
,
Xenbase Wang,
A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells.
1997,
Pubmed
,
Xenbase Waskiewicz,
Mitogen and stress response pathways: MAP kinase cascades and phosphatase regulation in mammals and yeast.
1995,
Pubmed Wells,
The spindle-assembly checkpoint: aiming for a perfect mitosis, every time.
1996,
Pubmed Yew,
Meiotic initiation by the mos protein in Xenopus.
1992,
Pubmed
,
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