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Mol Biol Cell
1997 Oct 01;810:2077-88. doi: 10.1091/mbc.8.10.2077.
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The involvement of the intermediate chain of cytoplasmic dynein in binding the motor complex to membranous organelles of Xenopus oocytes.
Steffen W, Karki S, Vaughan KT, Vallee RB, Holzbaur EL, Weiss DG, Kuznetsov SA.
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Cytoplasmic dynein is one of the major motor proteins involved in intracellular transport. It is a protein complex consisting of four subunit classes: heavy chains, intermediate chains (ICs), light intermediate chains, and light chains. In a previous study, we had generated new monoclonal antibodies to the ICs and mapped the ICs to the base of the motor. Because the ICs have been implicated in targeting the motor to cargo, we tested whether these new antibodies to the intermediate chain could block the function of cytoplasmic dynein. When cytoplasmic extracts of Xenopus oocytes were incubated with either one of the monoclonal antibodies (m74-1, m74-2), neither organelle movement nor network formation was observed. Network formation and membrane transport was blocked at an antibody concentration as low as 15 micrograms/ml. In contrast to these observations, no effect was observed on organelle movement and tubular network formation in the presence of a control antibody at concentrations as high as 0.5 mg/ml. After incubating cytoplasmic extracts or isolated membranes with the monoclonal antibodies m74-1 and m74-2, the dynein IC polypeptide was no longer detectable in the membrane fraction by SDS-PAGE immunoblot, indicating a loss of cytoplasmic dynein from the membrane. We used a panel of dynein IC truncation mutants and mapped the epitopes of both antibodies to the N-terminal coiled-coil domain, in close proximity to the p150Glued binding domain. In an IC affinity column binding assay, both antibodies inhibited the IC-p150Glued interaction. Thus these findings demonstrate that direct IC-p150Glued interaction is required for the proper attachment of cytoplasmic dynein to membranes.
Adams,
Binding of myosin I to membrane lipids.
1989, Pubmed
Adams,
Binding of myosin I to membrane lipids.
1989,
Pubmed Allan,
Movement of membrane tubules along microtubules in vitro: evidence for specialised sites of motor attachment.
1994,
Pubmed
,
Xenbase Allan,
Assay of membrane motility in interphase and metaphase Xenopus extracts.
1993,
Pubmed
,
Xenbase Allan,
Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro.
1995,
Pubmed
,
Xenbase Allan,
Cell cycle control of microtubule-based membrane transport and tubule formation in vitro.
1991,
Pubmed
,
Xenbase Allen,
Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: evidence for an active role of microtubules in cytoplasmic transport.
1985,
Pubmed Allen,
Video-enhanced microscopy with a computer frame memory.
1983,
Pubmed Allen,
Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: a new method capable of analyzing microtubule-related motility in the reticulopodial network of Allogromia laticollaris.
1981,
Pubmed Aniento,
Cytoplasmic dynein-dependent vesicular transport from early to late endosomes.
1993,
Pubmed Blocker,
Molecular requirements for bi-directional movement of phagosomes along microtubules.
1997,
Pubmed Blocker,
Microtubule-associated protein-dependent binding of phagosomes to microtubules.
1996,
Pubmed Collins,
Reversible assembly purification of taxol-treated microtubules.
1991,
Pubmed Corthésy-Theulaz,
Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex.
1992,
Pubmed Dabora,
The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts.
1988,
Pubmed Doberstein,
Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba myosin IC.
1992,
Pubmed Echeverri,
Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis.
1996,
Pubmed Fath,
Molecular motors are differentially distributed on Golgi membranes from polarized epithelial cells.
1994,
Pubmed Franke,
Cytoplasmic microtubules linked to endoplasmic reticulum with cross-bridges.
1971,
Pubmed Gill,
Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein.
1991,
Pubmed Hayden,
Binding of brush border myosin I to phospholipid vesicles.
1990,
Pubmed Hirokawa,
Brain dynein (MAP1C) localizes on both anterogradely and retrogradely transported membranous organelles in vivo.
1990,
Pubmed Holleran,
Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles.
1996,
Pubmed Karki,
Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex.
1995,
Pubmed King,
The Mr 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with alpha-tubulin in situ.
1991,
Pubmed Lacey,
Cytoplasmic dynein is a vesicle protein.
1992,
Pubmed Lacey,
Cytoplasmic dynein binds to phospholipid vesicles.
1994,
Pubmed Lee,
Dynamic behavior of endoplasmic reticulum in living cells.
1988,
Pubmed Lee,
Construction of the endoplasmic reticulum.
1989,
Pubmed Li,
Association of a cellular myosin II with anionic phospholipids and the neuronal plasma membrane.
1994,
Pubmed Lin,
Immunolocalization of cytoplasmic dynein to lysosomes in cultured cells.
1992,
Pubmed Mage,
Preparation of Fab fragments from IgGs of different animal species.
1980,
Pubmed Muresan,
Plus-end motors override minus-end motors during transport of squid axon vesicles on microtubules.
1996,
Pubmed Murray,
Cell cycle extracts.
1991,
Pubmed Niclas,
Cell cycle regulation of dynein association with membranes modulates microtubule-based organelle transport.
1996,
Pubmed
,
Xenbase Paschal,
Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function.
1992,
Pubmed Paschal,
MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties.
1987,
Pubmed Schroer,
Two activators of microtubule-based vesicle transport.
1991,
Pubmed Steffen,
Inhibition of Microtubule-Dependent, Minus-End Directed Transport of Axoplasmic Organelles by an Antibody Specific for the Intermediate Chain of Dynein.
1997,
Pubmed Steffen,
Immunogold localisation of the intermediate chain within the protein complex of cytoplasmic dynein.
1996,
Pubmed Steuer,
Localization of cytoplasmic dynein to mitotic spindles and kinetochores.
1990,
Pubmed Terasaki,
Interactions among endoplasmic reticulum, microtubules, and retrograde movements of the cell surface.
1994,
Pubmed
,
Xenbase Tokito,
Functionally distinct isoforms of dynactin are expressed in human neurons.
1996,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed Toyoshima,
Kinectin, a major kinesin-binding protein on ER.
1992,
Pubmed Vaisberg,
Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.
1993,
Pubmed Vaisberg,
Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles.
1996,
Pubmed Vale,
Formation of membrane networks in vitro by kinesin-driven microtubule movement.
1988,
Pubmed Vaughan,
Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued.
1995,
Pubmed Waterman-Storer,
The interaction between cytoplasmic dynein and dynactin is required for fast axonal transport.
1997,
Pubmed Waterman-Storer,
The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1).
1995,
Pubmed Yu,
Kinesin and cytoplasmic dynein binding to brain microsomes.
1992,
Pubmed
