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Acute morphogenic and chemotropic effects of neurotrophins on cultured embryonic Xenopus spinal neurons.
Ming Gl, Lohof AM, Zheng JQ.
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Neurotrophins constitute a family of trophic factors with profound effects on the survival and differentiation of the nervous system. Addition of brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), but not nerve growth factor (NGF), increased the survival of embryonic Xenopus spinal neurons in culture, although all three neurotrophins enhanced neurite outgrowth. Here we report that neurotrophins also exert acute actions on the morphology and motility of 1-day-old cultured Xenopus spinal neurons. Bath application of BDNF induced extensive formation of lamellipodia simultaneously at multiple sites along the neurite shaft as well as at the growth cone. The BDNF-induced lamellipodia appeared within minutes, rapidly protruded to their greatest extent in about 10 min, and gradually disappeared thereafter, leaving behind newly formed thin lateral processes. When applied as microscopic concentration gradients, both BDNF and NT-3, but not NGF, induced the growth cone to grow toward the neurotrophin source. Our results suggest that neurotrophic factors, when delivered to responsive neurons, may serve as morphogenic and chemotropic agents during neuronal development.
Aletta,
Growth cone configuration and advance: a time-lapse study using video-enhanced differential interference contrast microscopy.
1988, Pubmed
Aletta,
Growth cone configuration and advance: a time-lapse study using video-enhanced differential interference contrast microscopy.
1988,
Pubmed Bailey,
Long-term sensitization in Aplysia increases the number of presynaptic contacts onto the identified gill motor neuron L7.
1988,
Pubmed Bailey,
Structural changes accompanying memory storage.
1993,
Pubmed Barbacid,
Neurotrophic factors and their receptors.
1995,
Pubmed Barbacid,
The Trk family of neurotrophin receptors.
1994,
Pubmed Barde,
Trophic factors and neuronal survival.
1989,
Pubmed Barde,
The nerve growth factor family.
1990,
Pubmed Barker,
Wortmannin blocks lipid and protein kinase activities associated with PI 3-kinase and inhibits a subset of responses induced by Fc epsilon R1 cross-linking.
1995,
Pubmed Berninger,
Fast actions of neurotrophic factors.
1996,
Pubmed Boulton,
ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.
1991,
Pubmed Bray,
Cortical flow in animal cells.
1988,
Pubmed Chao,
Neurotrophin receptors: a window into neuronal differentiation.
1992,
Pubmed Cohen-Cory,
Effects of brain-derived neurotrophic factor on optic axon branching and remodelling in vivo.
1995,
Pubmed
,
Xenbase Connolly,
Rapid regulation of neuronal growth cone shape and surface morphology by nerve growth factor.
1987,
Pubmed Connolly,
Regulation of growth cone morphology by nerve growth factor: a comparative study by scanning electron microscopy.
1985,
Pubmed Davies,
The role of neurotrophins in the developing nervous system.
1994,
Pubmed Davies,
Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor.
,
Pubmed Dechant,
Neurotrophin receptors.
1994,
Pubmed Desmond,
Changes in the postsynaptic density with long-term potentiation in the dentate gyrus.
1986,
Pubmed Ernfors,
Increased levels of messenger RNAs for neurotrophic factors in the brain during kindling epileptogenesis.
1991,
Pubmed Funakoshi,
Differential expression of mRNAs for neurotrophins and their receptors after axotomy of the sciatic nerve.
1993,
Pubmed Glanzman,
Target-dependent structural changes accompanying long-term synaptic facilitation in Aplysia neurons.
1990,
Pubmed Greene,
Early events in neurotrophin signalling via Trk and p75 receptors.
1995,
Pubmed Griffin,
Rapid retraction of neurites by sensory neurons in response to increased concentrations of nerve growth factor.
1980,
Pubmed Gundersen,
Characterization of the turning response of dorsal root neurites toward nerve growth factor.
1980,
Pubmed Halegoua,
Changes in the phosphorylation and distribution of vinculin during nerve growth factor induced neurite outgrowth.
1987,
Pubmed Hallböök,
Cellular localization of brain-derived neurotrophic factor and neurotrophin-3 mRNA expression in the early chicken embryo.
1993,
Pubmed Hallböök,
Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary.
1991,
Pubmed
,
Xenbase Henderson,
Neurotrophins promote motor neuron survival and are present in embryonic limb bud.
1993,
Pubmed Heumann,
Neurotrophin signalling.
1994,
Pubmed Hyman,
BDNF is a neurotrophic factor for dopaminergic neurons of the substantia nigra.
1991,
Pubmed Ip,
Neurotrophic factors and their receptors.
1995,
Pubmed Isackson,
BDNF mRNA expression is increased in adult rat forebrain after limbic seizures: temporal patterns of induction distinct from NGF.
1991,
Pubmed Kaplan,
Neurotrophin signal transduction by the Trk receptor.
1994,
Pubmed Kater,
Regulation of growth cone behavior by calcium.
1991,
Pubmed Kennedy,
Guidance and induction of branch formation in developing axons by target-derived diffusible factors.
1995,
Pubmed Klein,
Role of neurotrophins in mouse neuronal development.
1994,
Pubmed Knüsel,
K-252 compounds: modulators of neurotrophin signal transduction.
1992,
Pubmed Kotani,
Phosphoinositide 3-kinase as an upstream regulator of the small GTP-binding protein Rac in the insulin signaling of membrane ruffling.
1995,
Pubmed Kuffler,
Promoting and directing axon outgrowth.
1994,
Pubmed Letourneau,
Chemotactic response of nerve fiber elongation to nerve growth factor.
1978,
Pubmed Lewin,
Physiology of the neurotrophins.
1996,
Pubmed Lo,
Neurotrophic factors and synaptic plasticity.
1995,
Pubmed Lohof,
Potentiation of developing neuromuscular synapses by the neurotrophins NT-3 and BDNF.
1993,
Pubmed
,
Xenbase Lohof,
Asymmetric modulation of cytosolic cAMP activity induces growth cone turning.
1992,
Pubmed
,
Xenbase Lu,
Depolarizing stimuli regulate nerve growth factor gene expression in cultured hippocampal neurons.
1991,
Pubmed Lumsden,
Earliest sensory nerve fibres are guided to peripheral targets by attractants other than nerve growth factor.
,
Pubmed Lundborg,
Trophism, tropism, and specificity in nerve regeneration.
1994,
Pubmed Maisonpierre,
NT-3, BDNF, and NGF in the developing rat nervous system: parallel as well as reciprocal patterns of expression.
1990,
Pubmed Mason,
Growth cone form is behavior-specific and, consequently, position-specific along the retinal axon pathway.
1997,
Pubmed McAllister,
Neurotrophins regulate dendritic growth in developing visual cortex.
1995,
Pubmed McAllister,
Neurotrophin regulation of cortical dendritic growth requires activity.
1996,
Pubmed McTigue,
Nerve growth factor and other agents mediate phosphorylation and activation of tyrosine hydroxylase. A convergence of multiple kinase activities.
1985,
Pubmed Menesini Chen,
Sympathetic nerve fibers ingrowth in the central nervous system of neonatal rodent upon intracerebral NGF injections.
1978,
Pubmed Obermeier,
Neuronal differentiation signals are controlled by nerve growth factor receptor/Trk binding sites for SHC and PLC gamma.
1994,
Pubmed Obermeier,
Identification of Trk binding sites for SHC and phosphatidylinositol 3'-kinase and formation of a multimeric signaling complex.
1993,
Pubmed Ohmichi,
Activation of phosphatidylinositol-3 kinase by nerve growth factor involves indirect coupling of the trk proto-oncogene with src homology 2 domains.
1992,
Pubmed Parker,
Intracellular signalling. PI 3-kinase puts GTP on the Rac.
1995,
Pubmed Patterson,
Neurotrophin expression in rat hippocampal slices: a stimulus paradigm inducing LTP in CA1 evokes increases in BDNF and NT-3 mRNAs.
1992,
Pubmed Ruit,
Selective dependence of mammalian dorsal root ganglion neurons on nerve growth factor during embryonic development.
1992,
Pubmed Sato,
Action of a diffusible target-derived chemoattractant on cortical axon branch induction and directed growth.
1994,
Pubmed Schnell,
Neurotrophin-3 enhances sprouting of corticospinal tract during development and after adult spinal cord lesion.
1994,
Pubmed Seeley,
Short-latency local actions of nerve growth factor at the growth cone.
1983,
Pubmed Segal,
Changes in neurotrophin responsiveness during the development of cerebellar granule neurons.
1992,
Pubmed Segal,
Intracellular signaling pathways activated by neurotrophic factors.
1996,
Pubmed Small,
Organization of actin meshworks in cultured cells: the leading edge.
1982,
Pubmed Smith,
Neuronal cytomechanics: the actin-based motility of growth cones.
1988,
Pubmed Snider,
Neurotrophic molecules.
1989,
Pubmed Snider,
Functions of the neurotrophins during nervous system development: what the knockouts are teaching us.
1994,
Pubmed Song,
cAMP-induced switching in turning direction of nerve growth cones.
1997,
Pubmed
,
Xenbase Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase Stoop,
Synaptic modulation by neurotrophic factors: differential and synergistic effects of brain-derived neurotrophic factor and ciliary neurotrophic factor.
1996,
Pubmed
,
Xenbase Tanaka,
Making the connection: cytoskeletal rearrangements during growth cone guidance.
1995,
Pubmed Tapley,
K252a is a selective inhibitor of the tyrosine protein kinase activity of the trk family of oncogenes and neurotrophin receptors.
1992,
Pubmed Tessier-Lavigne,
The molecular biology of axon guidance.
1996,
Pubmed Thoenen,
Neurotrophins and neuronal plasticity.
1995,
Pubmed Vetter,
Nerve growth factor rapidly stimulates tyrosine phosphorylation of phospholipase C-gamma 1 by a kinase activity associated with the product of the trk protooncogene.
1991,
Pubmed Wennström,
Activation of phosphoinositide 3-kinase is required for PDGF-stimulated membrane ruffling.
1994,
Pubmed Zhang,
NGF and NT-3 have differing effects on the growth of dorsal root axons in developing mammalian spinal cord.
1994,
Pubmed Zheng,
Turning of nerve growth cones induced by neurotransmitters.
1994,
Pubmed
,
Xenbase Zheng,
Essential role of filopodia in chemotropic turning of nerve growth cone induced by a glutamate gradient.
1996,
Pubmed
,
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