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Assembly of a mitotic spindle requires the accurate regulation of microtubule dynamics which is accomplished, at least in part, by phosphorylation-dephosphorylation reactions. Here we have investigated the role of serine-threonine phosphatases in the control of microtubule dynamics using specific inhibitors in Xenopus egg extracts. Type 2A phosphatases are required to maintain the short steady-state length of microtubules in mitosis by regulating the level of microtubule catastrophes, in part by controlling the the microtubule-destabilizing activity and phosphorylation of Op18/stathmin. Type 1 phosphatases are only required for control of microtubule dynamics during the transitions into and out of mitosis. Thus, although both type 2A and type 1 phosphatases are involved in the regulation of microtubule dynamics, they have distinct, non-overlapping roles.
Armstrong,
Drosophila PPY, a novel male specific protein serine/threonine phosphatase localised in somatic cells of the testis.
1995, Pubmed
Armstrong,
Drosophila PPY, a novel male specific protein serine/threonine phosphatase localised in somatic cells of the testis.
1995,
Pubmed Ault,
Studies on the ejection properties of asters: astral microtubule turnover influences the oscillatory behavior and positioning of mono-oriented chromosomes.
1991,
Pubmed Axton,
One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis.
1990,
Pubmed Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase Black,
A regulated MET3-GLC7 gene fusion provides evidence of a mitotic role for Saccharomyces cerevisiae protein phosphatase 1.
1995,
Pubmed Booher,
Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control.
1989,
Pubmed Brattsand,
Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38.
1994,
Pubmed Brewis,
PPX, a novel protein serine/threonine phosphatase localized to centrosomes.
1993,
Pubmed Chen,
A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus.
1994,
Pubmed Clarke,
Dephosphorylation of cdc25-C by a type-2A protein phosphatase: specific regulation during the cell cycle in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase Cohen,
The structure and regulation of protein phosphatases.
1989,
Pubmed de Pennart,
Okadaic acid induces spindle lengthening and disrupts the interaction of microtubules with the kinetochores in metaphase II-arrested mouse oocytes.
1993,
Pubmed Dogterom,
Influence of M-phase chromatin on the anisotropy of microtubule asters.
1996,
Pubmed
,
Xenbase Dohadwala,
Phosphorylation and inactivation of protein phosphatase 1 by cyclin-dependent kinases.
1994,
Pubmed Evans,
Mutations in the Saccharomyces cerevisiae type 2A protein phosphatase catalytic subunit reveal roles in cell wall integrity, actin cytoskeleton organization and mitosis.
1997,
Pubmed Felix,
A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
1989,
Pubmed
,
Xenbase Fernandez,
Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit.
1992,
Pubmed Ferrigno,
Protein phosphatase 2A1 is the major enzyme in vertebrate cell extracts that dephosphorylates several physiological substrates for cyclin-dependent protein kinases.
1993,
Pubmed
,
Xenbase Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Gomes,
Abnormal anaphase resolution (aar): a locus required for progression through mitosis in Drosophila.
1993,
Pubmed Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Hisamoto,
The Glc7 type 1 protein phosphatase of Saccharomyces cerevisiae is required for cell cycle progression in G2/M.
1994,
Pubmed Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase Honkanen,
Identification, purification, and characterization of a novel serine/threonine protein phosphatase from bovine brain.
1991,
Pubmed Horwitz,
The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation.
1997,
Pubmed Hyman,
Preparation of modified tubulins.
1991,
Pubmed Kinoshita,
Negative regulation of mitosis by the fission yeast protein phosphatase ppa2.
1993,
Pubmed Larsson,
G2/M transition requires multisite phosphorylation of oncoprotein 18 by two distinct protein kinase systems.
1995,
Pubmed Lee,
Inhibition of cdc2 activation by INH/PP2A.
1994,
Pubmed
,
Xenbase Lorca,
Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs.
1993,
Pubmed
,
Xenbase MAQSOOD,
Radioisotopes in medicine.
1957,
Pubmed Marklund,
Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics.
1996,
Pubmed Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase Ohkura,
The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
1989,
Pubmed Parsons,
Microtubule assembly in clarified Xenopus egg extracts.
1997,
Pubmed
,
Xenbase Picard,
Okadaic acid mimics a nuclear component required for cyclin B-cdc2 kinase microinjection to drive starfish oocytes into M phase.
1991,
Pubmed
,
Xenbase Picard,
Involvement of protein phosphatases 1 and 2A in the control of M phase-promoting factor activity in starfish.
1989,
Pubmed Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase Shamu,
Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase.
1992,
Pubmed
,
Xenbase Shenolikar,
Protein serine/threonine phosphatases--new avenues for cell regulation.
1994,
Pubmed Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase Snaith,
Deficiency of protein phosphatase 2A uncouples the nuclear and centrosome cycles and prevents attachment of microtubules to the kinetochore in Drosophila microtubule star (mts) embryos.
1996,
Pubmed Sobel,
Stathmin: a relay phosphoprotein for multiple signal transduction?
1991,
Pubmed Sontag,
A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle.
1995,
Pubmed Surana,
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
1993,
Pubmed Van Dolah,
Okadaic acid inhibits a protein phosphatase activity involved in formation of the mitotic spindle of GH4 rat pituitary cells.
1992,
Pubmed Verde,
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
1990,
Pubmed
,
Xenbase Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Walczak,
XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
1996,
Pubmed
,
Xenbase Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed Wells,
The spindle-assembly checkpoint: aiming for a perfect mitosis, every time.
1996,
Pubmed Yamano,
Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation.
1994,
Pubmed Zhai,
Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis.
1996,
Pubmed Zhang,
The impact of chromosomes and centrosomes on spindle assembly as observed in living cells.
1995,
Pubmed
