Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
CENP-E, a kinesin-like protein that is known to associate with kinetochores during all phases of mitotic chromosome movement, is shown here to be a component of meiotic kinetochores as well. CENP-E is detected at kinetochores during metaphase I in both mice and frogs, and, as in mitosis, is relocalized to the midbody during telophase. CENP-E function is essential for meiosis I because injection of an antibody to CENP-E into mouse oocytes in prophase completely prevented progression of those oocytes past metaphase I. Beyond this, CENP-E is modified or masked during the natural, Mos-dependent, cell cycle arrest that occurs at metaphase II, although it is readily detectable at the kinetochores in metaphase II oocytes derived from mos-deficient (MOS-/-) mice that fail to arrest at metaphase II. This must reflect a masking of some CENP-E epitopes, not the absence of CENP-E, in meiosis II because a different polyclonal antibody raised to the tail of CENP-E detects CENP-E at kinetochores of metaphase II-arrested eggs and because CENP-E reappears in telophase of mouse oocytes activated in the absence of protein synthesis.
Barrett,
Ha-rasVal-12,Thr-59 activates S6 kinase and p34cdc2 kinase in Xenopus oocytes: evidence for c-mosxe-dependent and -independent pathways.
1990, Pubmed,
Xenbase
Barrett,
Ha-rasVal-12,Thr-59 activates S6 kinase and p34cdc2 kinase in Xenopus oocytes: evidence for c-mosxe-dependent and -independent pathways.
1990,
Pubmed
,
Xenbase Brown,
The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A.
1996,
Pubmed Brown,
Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis.
1994,
Pubmed Campbell,
Microinjection of mitotic cells with the 3F3/2 anti-phosphoepitope antibody delays the onset of anaphase.
1995,
Pubmed Choi,
Activation of p34cdc2 protein kinase activity in meiotic and mitotic cell cycles in mouse oocytes and embryos.
1991,
Pubmed Choi,
The Mos/mitogen-activated protein kinase (MAPK) pathway regulates the size and degradation of the first polar body in maturing mouse oocytes.
1996,
Pubmed
,
Xenbase Choi,
Mos/mitogen-activated protein kinase can induce early meiotic phenotypes in the absence of maturation-promoting factor: a novel system for analyzing spindle formation during meiosis I.
1996,
Pubmed Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase Duesbery,
The role of Ca(2+) in progesterone-induced germinal vesicle breakdown of Xenopus laevis oocytes: the synergic effects of microtubule depolymerization and Ca(2+).
1996,
Pubmed
,
Xenbase Elinson,
A transient array of parallel microtubules in frog eggs: potential tracks for a cytoplasmic rotation that specifies the dorso-ventral axis.
1988,
Pubmed
,
Xenbase Freeman,
Xenopus homolog of the mos protooncogene transforms mammalian fibroblasts and induces maturation of Xenopus oocytes.
1989,
Pubmed
,
Xenbase Fukasawa,
Mos overexpression in Swiss 3T3 cells induces meiotic-like alterations of the mitotic spindle.
1995,
Pubmed Gard,
Microtubule organization during maturation of Xenopus oocytes: assembly and rotation of the meiotic spindles.
1992,
Pubmed
,
Xenbase Gorbsky,
Differential expression of a phosphoepitope at the kinetochores of moving chromosomes.
1993,
Pubmed Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase Hardwick,
Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption.
1996,
Pubmed Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase Hyman,
Morphogenetic properties of microtubules and mitotic spindle assembly.
1996,
Pubmed Li,
Identification of a human mitotic checkpoint gene: hsMAD2.
1996,
Pubmed Li,
Mitotic forces control a cell-cycle checkpoint.
1995,
Pubmed Liao,
Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein.
1994,
Pubmed Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase Lombillo,
Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.
1995,
Pubmed Mansour,
Transformation of mammalian cells by constitutively active MAP kinase kinase.
1994,
Pubmed Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed McKim,
Mechanical basis of meiotic metaphase arrest.
1993,
Pubmed Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Moses,
Cytostatic factor (CSF) in the eggs of Xenopus laevis.
1989,
Pubmed
,
Xenbase Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase Nicklas,
Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint.
1995,
Pubmed O'Keefe,
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
1989,
Pubmed Paules,
Mouse Mos protooncogene product is present and functions during oogenesis.
1989,
Pubmed
,
Xenbase Pluta,
The centromere: hub of chromosomal activities.
1995,
Pubmed Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Rieder,
The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores.
1995,
Pubmed Roy,
The cyclin B2 component of MPF is a substrate for the c-mos(xe) proto-oncogene product.
1990,
Pubmed
,
Xenbase Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sawin,
Meiosis, mitosis and microtubule motors.
1993,
Pubmed
,
Xenbase Shibuya,
Activation of p42 MAP kinase and the release of oocytes from cell cycle arrest.
1992,
Pubmed Siracusa,
Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis.
1978,
Pubmed Surana,
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
1993,
Pubmed Thrower,
Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor.
1995,
Pubmed Wang,
Mos oncogene product associates with kinetochores in mammalian somatic cells and disrupts mitotic progression.
1994,
Pubmed
,
Xenbase Yen,
CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase.
1991,
Pubmed Yen,
CENP-E is a putative kinetochore motor that accumulates just before mitosis.
1992,
Pubmed Yen,
Kinetochore function: molecular motors, switches and gates.
1996,
Pubmed Yew,
Meiotic initiation by the mos protein in Xenopus.
1992,
Pubmed
,
Xenbase Zhang,
Activation of Xenopus laevis eggs in the absence of intracellular Ca activity by the protein phosphorylation inhibitor, 6-dimethylaminopurine (6-DMAP).
1992,
Pubmed
,
Xenbase Zhou,
In vitro and in vivo characterization of pp39mos association with tubulin.
1991,
Pubmed
