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EMBO J
1997 Aug 15;1616:4817-25. doi: 10.1093/emboj/16.16.4817.
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Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1.
Loukin SH, Vaillant B, Zhou XL, Spalding EP, Kung C, Saimi Y.
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YKC1 (TOK1, DUK1, YORK) encodes the outwardly rectifying K+ channel of the yeast plasma membrane. Non-targeted mutations of YKC1 were isolated by their ability to completely block proliferation when expressed in yeast. All such mutations examined occurred near the cytoplasmic ends of the transmembrane segments following either of the duplicated P loops, which we termed the 'post-P loop' (PP) regions. These PP mutations specifically caused marked defects in the 'C1' states, a set of interrelated closed states that Ykc1 enters and exits at rates of tens to hundreds of milliseconds. These results indicate that the Ykc1 PP region plays a role in determining closed state conformations and that non-targeted mutagenesis and microbial selection can be a valuable tool for probing structure-function relationships of ion channels.
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