Hatada S et al. (1997), A novel gene encoding a ferredoxin reductase-li...

XB-ART-16223

Gene 1997 Jul 31;1942:297-9. doi: 10.1016/s0378-1119(97)00208-4.

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A novel gene encoding a ferredoxin reductase-like protein expressed in the neuroectoderm in Xenopus neurula.

Hatada S, Kinoshita M, Sakumoto H, Nishihara R, Noda M, Asashima M.

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In an attempt to elucidate the molecular mechanisms of early neural development in Xenopus laevis, we identified, using a differential display method, several genes that are induced after Concanavalin A treatment in the animal caps prepared from stage 9 blastula. One such gene was found to encode a possible type IIIa membrane protein of 66.2 kDa sharing similarities with several prokaryotic and eukaryotic redox enzymes, hence the putative product was named Nfrl, neurula-specific ferredoxin reductase-like protein. Northern blot analysis confirmed that the expression of the Nfrl gene is up-regulated around the neurula stage, and is much lower in embryos of earlier stages and in adult tissues. The temporally limited expression of this gene implies neurula- and early larva-specific redox reactions of certain substrates, the nature of which remains to be elucidated.

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Species referenced: Xenopus laevis
Genes referenced: aifm3

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	Fig. 1. Structure of the Nfrl cDNA and its predicted amino acid sequence. The initial sequence (B1) amplified by differential display is underlined.
	Fig. 2. Northern blot analysis of the Nfrl gene. Each lane contained 5 mg of poly (A)+ RNA isolated from whole embryos at the indicated stage. The quality and the quantity of the RNA loaded were assessed by 28S and 18S ribosomal RNAs stained with ethidium bromide.