Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 Apr 15;948:3736-41. doi: 10.1073/pnas.94.8.3736.
Show Gene links
Show Anatomy links
RanBP2 associates with Ubc9p and a modified form of RanGAP1.
Saitoh H, Pu R, Cavenagh M, Dasso M.
???displayArticle.abstract???
Ran is a small GTPase required for nuclear transport in eukaryotic cells [Gorlich, D. & Mattaj, I. W. (1996) Science 271, 1513-1518]. Mutants in Ran also show defects in mRNA processing, cell cycle regulation, and other aspects of nuclear function [Rush, M. G., Drivas, G. & D'Eustachio, P. (1996) BioEssays 18, 103-112; Sazer, S. (1996) Trends Cell Biol. 6, 81-85]. In an effort to understand the role of Ran in these diverse processes, we previously characterized 10 Ran interacting proteins (Rips) from Xenopus egg extracts. In this report, we present further characterization of a complex containing three of these Rips: p340(RanBP2), p88, and p18. We have cloned the Xenopus homologue of RanGAP1, and we show here that p88 is a modified form of this protein. In RanGAP assays, the p340(RanBP2)-p88-p18 complex contains GTPase-activating protein activity, indicating that RanGAP1 is not inactivated by modification. Rather, modification of RanGAP1 appears to be linked to its association with p340(RanBP2) because we did not observe unmodified RanGAP1 in p340(RanBP2) immunoprecipitates. We have also characterized p18, and we found that it is the Xenopus homologue of Ubc9p, an E2 ubiquitin-conjugating enzyme that is required for cell cycle regulation [Seufert, W., Futcher, B. & Jentsch, S. (1995) Nature (London) 373, 78-81]. Using antibodies directed against Xenopus Ubc9p, we have confirmed that Ubc9p associates with p340(RanBP2) in Xenopus extracts. These results suggest Ubc9p's role in cell cycle regulation may involve either modification of nuclear transport substrates or the nuclear transport machinery.
Bischoff,
Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
1995, Pubmed
Bischoff,
Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
1995,
Pubmed Bischoff,
RanGAP1 induces GTPase activity of nuclear Ras-related Ran.
1994,
Pubmed Boddy,
PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia.
1996,
Pubmed Ciechanover,
The ubiquitin-proteasome proteolytic pathway.
1994,
Pubmed Clarke,
Regulation of Cdc2/cyclin B activation by Ran, a Ras-related GTPase.
1995,
Pubmed
,
Xenbase Dasso,
The role of the Ran GTPase pathway in cell cycle control and interphase nuclear functions.
1995,
Pubmed DeGregori,
A murine homolog of the yeast RNA1 gene is required for postimplantation development.
1994,
Pubmed Forrester,
Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure.
1992,
Pubmed Görlich,
Nucleocytoplasmic transport.
1996,
Pubmed Hateboer,
mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect.
1996,
Pubmed Jiang,
Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae.
1996,
Pubmed King,
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.
1995,
Pubmed
,
Xenbase Kornbluth,
Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression.
1994,
Pubmed
,
Xenbase Kovalenko,
Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.
1996,
Pubmed Loeb,
The yeast nuclear import receptor is required for mitosis.
1995,
Pubmed Mahajan,
A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2.
1997,
Pubmed Matunis,
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
1996,
Pubmed
,
Xenbase Melchior,
GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.
1995,
Pubmed Nishimoto,
Premature of chromosome condensation in a ts DNA- mutant of BHK cells.
1978,
Pubmed Nishitani,
Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.
1991,
Pubmed Rush,
The small nuclear GTPase Ran: how much does it run?
1996,
Pubmed Saitoh,
Direct and indirect association of the small GTPase ran with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts.
1996,
Pubmed
,
Xenbase Sazer,
The search for the primary function of the Ran GTPase continues.
1996,
Pubmed Seufert,
Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins.
1995,
Pubmed
,
Xenbase Shen,
UBL1, a human ubiquitin-like protein associating with human RAD51/RAD52 proteins.
1996,
Pubmed Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed Yasugi,
Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9.
1996,
Pubmed Yokoyama,
A giant nucleopore protein that binds Ran/TC4.
1995,
Pubmed
