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Electrostatic interactions between transmembrane segments mediate folding of Shaker K+ channel subunits.
Tiwari-Woodruff SK, Schulteis CT, Mock AF, Papazian DM.
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In voltage-dependent Shaker K+ channels, charged residues E293 in transmembrane segment S2 and R365, R368, and R371 in S4 contribute significantly to the gating charge movement that accompanies activation. Using an intragenic suppression strategy, we have now probed for structural interaction between transmembrane segments S2, S3, and S4 in Shaker channels. Charge reversal mutations of E283 in S2 and K374 in S4 disrupt maturation of the protein. Maturation was specifically and efficiently rescued by second-site charge reversal mutations, indicating that electrostatic interactions exist between E283 in S2 and R368 and R371 in S4, and between K374 in S4 and E293 in S2 and D316 in S3. Rescued subunits were incorporated into functional channels, demonstrating that a native structure was restored. Our data indicate that K374 interacts with E293 and D316 within the same subunit. These electrostatic interactions mediate the proper folding of the protein and are likely to persist in the native structure. Our results raise the possibility that the S4 segment is tilted relative to S2 and S3 in the voltage-sensing domain of Shaker channels. Such an arrangement might provide solvent access to voltage-sensing residues, which we find to be highly tolerant of mutations.
Aggarwal,
Contribution of the S4 segment to gating charge in the Shaker K+ channel.
1996, Pubmed,
Xenbase
Aggarwal,
Contribution of the S4 segment to gating charge in the Shaker K+ channel.
1996,
Pubmed
,
Xenbase Anderson,
pH-induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme.
1990,
Pubmed Bezanilla,
Inactivation of the sodium channel. I. Sodium current experiments.
1977,
Pubmed Bezanilla,
Gating of Shaker K+ channels: II. The components of gating currents and a model of channel activation.
1994,
Pubmed
,
Xenbase Catterall,
Molecular properties of voltage-sensitive sodium channels.
1986,
Pubmed Cosson,
Membrane protein association by potential intramembrane charge pairs.
1991,
Pubmed Demo,
The inactivation gate of the Shaker K+ channel behaves like an open-channel blocker.
1991,
Pubmed di Rago,
Genetic analysis of the folded structure of yeast mitochondrial cytochrome b by selection of intragenic second-site revertants.
1995,
Pubmed di Rago,
Intragenic suppressors reveal long distance interactions between inactivating and reactivating amino acid replacements generating three-dimensional constraints in the structure of mitochondrial cytochrome b.
1990,
Pubmed Durell,
Atomic scale structure and functional models of voltage-gated potassium channels.
1992,
Pubmed Fersht,
Conformational equilibria in -and -chymotrypsin. The energetics and importance of the salt bridge.
1972,
Pubmed Gething,
Protein folding in the cell.
1992,
Pubmed Goldstein,
A structural vignette common to voltage sensors and conduction pores: canaliculi.
1996,
Pubmed Guy,
Pursuing the structure and function of voltage-gated channels.
1990,
Pubmed
,
Xenbase Hartzog,
Second-site suppressor mutations at glycine 218 and histidine 245 in the alpha subunit of F1F0 ATP synthase in Escherichia coli.
1994,
Pubmed Hendsch,
Do salt bridges stabilize proteins? A continuum electrostatic analysis.
1994,
Pubmed Hendsch,
Protein stabilization by removal of unsatisfied polar groups: computational approaches and experimental tests.
1996,
Pubmed Horton,
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
1989,
Pubmed Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase Isacoff,
Putative receptor for the cytoplasmic inactivation gate in the Shaker K+ channel.
1991,
Pubmed
,
Xenbase King,
The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli.
1991,
Pubmed Landt,
A general method for rapid site-directed mutagenesis using the polymerase chain reaction.
1990,
Pubmed Larsson,
Transmembrane movement of the shaker K+ channel S4.
1996,
Pubmed
,
Xenbase Lee,
Possible salt bridges between transmembrane alpha-helices of the lactose carrier of Escherichia coli.
1992,
Pubmed Lemmon,
Specificity and promiscuity in membrane helix interactions.
1994,
Pubmed Lemmon,
Glycophorin A dimerization is driven by specific interactions between transmembrane alpha-helices.
1992,
Pubmed Lemmon,
Sequence specificity in the dimerization of transmembrane alpha-helices.
1992,
Pubmed Lemmon,
A dimerization motif for transmembrane alpha-helices.
1994,
Pubmed Li,
Images of purified Shaker potassium channels.
1994,
Pubmed
,
Xenbase Liu,
Intragenic suppression among CDC34 (UBC3) mutations defines a class of ubiquitin-conjugating catalytic domains.
1995,
Pubmed MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase MacKinnon,
Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels.
1990,
Pubmed MacKinnon,
Functional stoichiometry of Shaker potassium channel inactivation.
1993,
Pubmed
,
Xenbase Mannuzzu,
Direct physical measure of conformational rearrangement underlying potassium channel gating.
1996,
Pubmed
,
Xenbase Meeker,
Contributions of the ionizable amino acids to the stability of staphylococcal nuclease.
1996,
Pubmed Nakamura,
Roles of electrostatic interaction in proteins.
1996,
Pubmed Oliveberg,
New approach to the study of transient protein conformations: the formation of a semiburied salt link in the folding pathway of barnase.
1996,
Pubmed Papazian,
Electrostatic interactions of S4 voltage sensor in Shaker K+ channel.
1995,
Pubmed
,
Xenbase Papazian,
Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence.
1991,
Pubmed
,
Xenbase Peled-Zehavi,
Coassembly of synthetic segments of shaker K+ channel within phospholipid membranes.
1996,
Pubmed Perozo,
S4 mutations alter gating currents of Shaker K channels.
1994,
Pubmed
,
Xenbase Sahin-Tóth,
Properties of interacting aspartic acid and lysine residues in the lactose permease of Escherichia coli.
1993,
Pubmed Schoppa,
The size of gating charge in wild-type and mutant Shaker potassium channels.
1992,
Pubmed Schulteis,
Intersubunit interaction between amino- and carboxyl-terminal cysteine residues in tetrameric shaker K+ channels.
1996,
Pubmed
,
Xenbase Schulteis,
Conserved cysteine residues in the shaker K+ channel are not linked by a disulfide bond.
1995,
Pubmed
,
Xenbase Schwarz,
Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila.
1988,
Pubmed Seoh,
Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.
1996,
Pubmed
,
Xenbase Shortle,
Genetic analysis of staphylococcal nuclease: identification of three intragenic "global" suppressors of nuclease-minus mutations.
1985,
Pubmed Sigworth,
Voltage gating of ion channels.
1994,
Pubmed Timpe,
Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Tissot,
Importance of two buried salt bridges in the stability and folding pathway of barnase.
1996,
Pubmed Waldburger,
Are buried salt bridges important for protein stability and conformational specificity?
1995,
Pubmed Yang,
Evidence for voltage-dependent S4 movement in sodium channels.
1995,
Pubmed Yang,
Molecular basis of charge movement in voltage-gated sodium channels.
1996,
Pubmed Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed Zagotta,
Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB.
1990,
Pubmed
,
Xenbase Zagotta,
Shaker potassium channel gating. III: Evaluation of kinetic models for activation.
1994,
Pubmed
,
Xenbase
