Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Regulation of quantal secretion from developing motoneurons by postsynaptic activity-dependent release of NT-3.
Liou JC, Fu WM.
???displayArticle.abstract???
Neurotrophic factors derived from postsynaptic muscle cells may play important roles in the development of presynaptic neuronal functions. In 3-d-old Xenopus nerve-muscle cultures, embryonic spinal neurons that had made natural contact with co-cultured myocytes exhibited spontaneous release of larger packets of acetylcholine (ACh) quanta than those released by the isolated neurons having no contact with any myocyte. Treatment of isolated neurons with neurotrophin-3 (NT-3) for 2 d increased the average sizes of quantal ACh packets at newly formed nerve-muscle synapses, whereas treatment with antibody against NT-3 or with K252a, a specific inhibitor of tyrosine kinase receptors, decreased the quantal size at existing synapses, which suggests that NT-3 supplied by the postsynaptic muscle cell may be responsible for the development and maintenance of the quantal packets. The muscle effect seems to depend on synaptic activities mediated by postsynaptic ACh receptor channels, because chronic treatment of the culture with D-tubocurarine (D-Tc) for 2 d resulted in a marked reduction of the quantal sizes, when assayed after extensive washing of the culture with Ringer's solution. The curare treatment did not affect the postsynaptic ACh receptor sensitivity, because iontophoretically applied ACh induced current responses similar to those of control. Finally, co-treatment of the culture with NT-3 and D-Tc reversed the effect of D-Tc on the quantal size, and this reversal effect was abolished when K252a was also applied concomitantly. Our results suggest that muscle-derived NT-3 participates in the maturation of normal transmitter packets in developing neurons, and the secretion of NT-3 depends on spontaneous synaptic activity.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase Balice-Gordon,
Long-term synapse loss induced by focal blockade of postsynaptic receptors.
1994,
Pubmed Balice-Gordon,
In vivo observations of pre- and postsynaptic changes during the transition from multiple to single innervation at developing neuromuscular junctions.
1993,
Pubmed Barbacid,
Neurotrophic factors and their receptors.
1995,
Pubmed Bear,
Synaptic plasticity: LTP and LTD.
1994,
Pubmed Berninger,
Fast actions of neurotrophic factors.
1996,
Pubmed Buchanan,
Studies of nerve-muscle interactions in Xenopus cell culture: fine structure of early functional contacts.
1989,
Pubmed
,
Xenbase Chao,
Neurotrophin receptors: a window into neuronal differentiation.
1992,
Pubmed Chow,
Release of acetylcholine from embryonic neurons upon contact with muscle cell.
1985,
Pubmed
,
Xenbase Connor,
Retrograde signaling in the formation and maintenance of the neuromuscular junction.
1994,
Pubmed Cramer,
Activity-dependent remodeling of connections in the mammalian visual system.
1995,
Pubmed Dahm,
The regulation of synaptogenesis during normal development and following activity blockade.
1991,
Pubmed Dan,
Hebbian depression of isolated neuromuscular synapses in vitro.
1992,
Pubmed
,
Xenbase Dan,
Retrograde interactions during formation and elimination of neuromuscular synapses.
1994,
Pubmed Decker,
Calcium permeability of the nicotinic acetylcholine receptor: the single-channel calcium influx is significant.
1990,
Pubmed DiStefano,
The neurotrophins BDNF, NT-3, and NGF display distinct patterns of retrograde axonal transport in peripheral and central neurons.
1992,
Pubmed Eftimie,
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity.
1991,
Pubmed Englander,
Acetylcholine receptor clustering and nuclear movement in muscle fibers in culture.
1987,
Pubmed Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase Falls,
Mr 42,000 ARIA: a protein that may regulate the accumulation of acetylcholine receptors at developing chick neuromuscular junctions.
1990,
Pubmed Fischbach,
The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture.
1973,
Pubmed Fu,
Regulatory role of ATP at developing neuromuscular junctions.
1995,
Pubmed
,
Xenbase Funakoshi,
Muscle-derived neurotrophin-4 as an activity-dependent trophic signal for adult motor neurons.
1995,
Pubmed Goldman,
Acetylcholine receptor alpha-, beta-, gamma-, and delta-subunit mRNA levels are regulated by muscle activity.
1988,
Pubmed Goodman,
Developmental mechanisms that generate precise patterns of neuronal connectivity.
1993,
Pubmed Götz,
Neurotrophin-6 is a new member of the nerve growth factor family.
1994,
Pubmed Hall,
Synaptic structure and development: the neuromuscular junction.
1993,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Henderson,
Neurotrophins promote motor neuron survival and are present in embryonic limb bud.
1993,
Pubmed Heumann,
Neurotrophin signalling.
1994,
Pubmed Isackson,
Trophic factor response to neuronal stimuli or injury.
1995,
Pubmed Jasmin,
Golgi apparatus in chick skeletal muscle: changes in its distribution during end plate development and after denervation.
1989,
Pubmed Jennings,
Development of the neuromuscular synapse.
1993,
Pubmed Kidokoro,
Two types of miniature endplate potentials in Xenopus nerve-muscle cultures.
1984,
Pubmed
,
Xenbase Kidokoro,
Initial synaptic transmission at the growth cone in Xenopus nerve-muscle cultures.
1982,
Pubmed
,
Xenbase Kidokoro,
Changes in synaptic potential properties during acetylcholine receptor accumulation and neurospecific interactions in Xenopus nerve-muscle cell culture.
1980,
Pubmed
,
Xenbase Klein,
Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements.
1994,
Pubmed Koliatsos,
Evidence that brain-derived neurotrophic factor is a trophic factor for motor neurons in vivo.
1993,
Pubmed Korsching,
The neurotrophic factor concept: a reexamination.
1993,
Pubmed Lamballe,
trkC, a new member of the trk family of tyrosine protein kinases, is a receptor for neurotrophin-3.
1991,
Pubmed Laufer,
Activity-dependent regulation of gene expression in muscle and neuronal cells.
1989,
Pubmed Lo,
Heterosynaptic suppression of developing neuromuscular synapses in culture.
1994,
Pubmed
,
Xenbase Lo,
Activity-dependent synaptic competition in vitro: heterosynaptic suppression of developing synapses.
1991,
Pubmed
,
Xenbase Lohof,
Potentiation of developing neuromuscular synapses by the neurotrophins NT-3 and BDNF.
1993,
Pubmed
,
Xenbase Lu,
Exogenous synapsin I promotes functional maturation of developing neuromuscular synapses.
1992,
Pubmed
,
Xenbase Mason,
Release of the predicted calcitonin gene-related peptide from cultured rat trigeminal ganglion cells.
,
Pubmed Reist,
Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions.
1992,
Pubmed Sanes,
Activity-dependent accumulation of basal lamina by cultured rat myotubes.
1983,
Pubmed Snider,
Functions of the neurotrophins during nervous system development: what the knockouts are teaching us.
1994,
Pubmed Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase Takahashi,
Structure and physiology of developing neuromuscular synapses in culture.
1987,
Pubmed
,
Xenbase Wang,
Neurotrophins promote maturation of developing neuromuscular synapses.
1995,
Pubmed
,
Xenbase Wong,
The neurotrophins BDNF, NT-3 and NT-4/5, but not NGF, up-regulate the cholinergic phenotype of developing motor neurons.
1993,
Pubmed Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase Young,
Spontaneous release of transmitter from growth cones of embryonic neurones.
,
Pubmed
,
Xenbase Zafra,
Regulation of brain-derived neurotrophic factor and nerve growth factor mRNA in primary cultures of hippocampal neurons and astrocytes.
1992,
Pubmed Zafra,
Interplay between glutamate and gamma-aminobutyric acid transmitter systems in the physiological regulation of brain-derived neurotrophic factor and nerve growth factor synthesis in hippocampal neurons.
1991,
Pubmed Zafra,
Activity dependent regulation of BDNF and NGF mRNAs in the rat hippocampus is mediated by non-NMDA glutamate receptors.
1990,
Pubmed
