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Apoptosis is essential for the development and homeostasis of multicellular organisms. Recently, a cell-free extract prepared from Xenopus eggs was shown to recapitulate intracellular apoptotic pathways in vitro. While many stimuli have been shown to trigger apoptosis in a variety of cell types, the intracellular signaling pathways involved in apoptosis remain largely unknown. Here we show that addition of a recombinant protein containing the phosphotyrosine binding (SH2) domain from the adaptor protein crk, but not those derived from a panel of other signaling proteins, can prevent apoptosis in the Xenopus egg extract system. Furthermore, immunodepletion of endogenous crk protein from the egg extracts, or addition of anti-crk antisera to these extracts, prevents apoptosis. The ability to undergo apoptosis can be restored to these extracts by addition of recombinant crk protein. These results directly demonstrate that crk participates in apoptotic signaling.
Bakhshi,
Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18.
1985, Pubmed
Bakhshi,
Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18.
1985,
Pubmed Bellamy,
Cell death in health and disease: the biology and regulation of apoptosis.
1995,
Pubmed Boldin,
Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
1996,
Pubmed Chittenden,
Induction of apoptosis by the Bcl-2 homologue Bak.
1995,
Pubmed Cohen,
Modular binding domains in signal transduction proteins.
1995,
Pubmed Cortez,
Structural and signaling requirements for BCR-ABL-mediated transformation and inhibition of apoptosis.
1995,
Pubmed Cosulich,
Bcl-2 regulates activation of apoptotic proteases in a cell-free system.
1996,
Pubmed
,
Xenbase Ellis,
Mechanisms and functions of cell death.
1991,
Pubmed Farrow,
Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K.
1995,
Pubmed Feller,
c-Abl kinase regulates the protein binding activity of c-Crk.
1994,
Pubmed Fernandez-Sarabia,
Bcl-2 associates with the ras-related protein R-ras p23.
1993,
Pubmed Gulbins,
FAS-induced apoptosis is mediated via a ceramide-initiated RAS signaling pathway.
1995,
Pubmed Hockenbery,
Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.
1990,
Pubmed Hsueh,
Ovarian follicle atresia: a hormonally controlled apoptotic process.
1994,
Pubmed Kiefer,
Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.
1995,
Pubmed Knudsen,
Four proline-rich sequences of the guanine-nucleotide exchange factor C3G bind with unique specificity to the first Src homology 3 domain of Crk.
1994,
Pubmed Liu,
Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c.
1996,
Pubmed Liu,
Evidence for involvement of tyrosine phosphorylation in taxol-induced apoptosis in a human ovarian tumor cell line.
1994,
Pubmed Matsuda,
CRK protein binds to two guanine nucleotide-releasing proteins for the Ras family and modulates nerve growth factor-induced activation of Ras in PC12 cells.
1994,
Pubmed Matsuda,
Two species of human CRK cDNA encode proteins with distinct biological activities.
1992,
Pubmed Mayer,
A novel viral oncogene with structural similarity to phospholipase C.
1988,
Pubmed Mayer,
Mutagenic analysis of the v-crk oncogene: requirement for SH2 and SH3 domains and correlation between increased cellular phosphotyrosine and transformation.
1990,
Pubmed McGahon,
Regulation of the Fas apoptotic cell death pathway by Abl.
1995,
Pubmed Migita,
Prevention of anti-CD3 monoclonal antibody-induced thymic apoptosis by protein tyrosine kinase inhibitors.
1994,
Pubmed Muzio,
FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.
1996,
Pubmed Newmeyer,
Cell-free apoptosis in Xenopus egg extracts: inhibition by Bcl-2 and requirement for an organelle fraction enriched in mitochondria.
1994,
Pubmed
,
Xenbase Nichols,
Identification of CRKL as the constitutively phosphorylated 39-kD tyrosine phosphoprotein in chronic myelogenous leukemia cells.
1994,
Pubmed Pawson,
Protein modules and signalling networks.
1995,
Pubmed Reichman,
The product of the cellular crk gene consists primarily of SH2 and SH3 regions.
1992,
Pubmed Rosen,
Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein.
1995,
Pubmed Sato,
FAP-1: a protein tyrosine phosphatase that associates with Fas.
1995,
Pubmed Schaller,
pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk.
1995,
Pubmed Schieven,
Lineage-specific induction of B cell apoptosis and altered signal transduction by the phosphotyrosine phosphatase inhibitor bis(maltolato)oxovanadium(IV).
1995,
Pubmed Songyang,
SH2 domains recognize specific phosphopeptide sequences.
1993,
Pubmed Steller,
Mechanisms and genes of cellular suicide.
1995,
Pubmed Su,
Defective expression of hematopoietic cell protein tyrosine phosphatase (HCP) in lymphoid cells blocks Fas-mediated apoptosis.
1995,
Pubmed Tanaka,
C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the Src homology 3 domains of CRK and GRB2/ASH proteins.
1994,
Pubmed Tanaka,
Differential inhibition of signaling pathways by dominant-negative SH2/SH3 adapter proteins.
1995,
Pubmed Tilly,
Involvement of apoptosis in ovarian follicular atresia and postovulatory regression.
1991,
Pubmed Vaux,
Prevention of programmed cell death in Caenorhabditis elegans by human bcl-2.
1992,
Pubmed Vuori,
Introduction of p130cas signaling complex formation upon integrin-mediated cell adhesion: a role for Src family kinases.
1996,
Pubmed Wang,
R-Ras promotes apoptosis caused by growth factor deprivation via a Bcl-2 suppressible mechanism.
1995,
Pubmed Yousefi,
Protein-tyrosine phosphorylation regulates apoptosis in human eosinophils and neutrophils.
1994,
Pubmed
