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XB-ART-17555
Cell 1996 Oct 18;872:287-96. doi: 10.1016/s0092-8674(00)81346-x.
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Interaction between the origin recognition complex and the replication licensing system in Xenopus.

Rowles A, Chong JP, Brown L, Howell M, Evan GI, Blow JJ.


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The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.

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Species referenced: Xenopus laevis
Genes referenced: orc1 rlf
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