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A cell-free system derived from Xenopus eggs was used to identify the 'destruction box' of the Schizosaccharomyces pombe B-type cyclin, Cdc13, as residues 59-67: RHALDDVSN. Expression of indestructible Cdc13 from a regulated promoter in S.pombe blocked cells in anaphase and inhibited septation, showing that destruction of Cdc13 is necessary for exit from mitosis, but not for sister chromatid separation. In contrast, strong expression of a polypeptide comprising the N-terminal 70 residues of Cdc13, which acts as a competitive inhibitor of destruction box-mediated proteolysis, inhibited both sister chromatid separation and the destruction of Cdc13, whereas an equivalent construct with a mutated destruction box did not. Appropriately timed expression of this N-terminal fragment of Cdc13 overcame the G1 arrest seen in cdc10 mutant strains, suggesting that proteins required for the initiation of S phase are subject to destruction by the same proteolytic machinery as cyclin.
Basi,
TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.
1993, Pubmed
Basi,
TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.
1993,
Pubmed Booher,
The fission yeast cdc2/cdc13/suc1 protein kinase: regulation of catalytic activity and nuclear localization.
1989,
Pubmed Booher,
Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules.
1988,
Pubmed Brown,
Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis.
1994,
Pubmed Fantes,
Epistatic gene interactions in the control of division in fission yeast.
1979,
Pubmed Funabiki,
Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.
1993,
Pubmed Funabiki,
Cut2 proteolysis required for sister-chromatid seperation in fission yeast.
1996,
Pubmed Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Gordon,
A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition.
1996,
Pubmed Gordon,
Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit.
1993,
Pubmed Hagan,
The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.
1988,
Pubmed Hayles,
Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex.
1994,
Pubmed Hayles,
A pre-start checkpoint preventing mitosis in fission yeast acts independently of p34cdc2 tyrosine phosphorylation.
1995,
Pubmed Heichman,
The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle.
1996,
Pubmed Hershko,
Methylated ubiquitin inhibits cyclin degradation in clam embryo extracts.
1991,
Pubmed Hirano,
A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase.
1988,
Pubmed Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase Hunt,
The requirements for protein synthesis and degradation, and the control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo.
1992,
Pubmed Hunt,
Cyclins and their partners: from a simple idea to complicated reality.
1991,
Pubmed Irniger,
Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast.
1995,
Pubmed Kelly,
The fission yeast cdc18+ gene product couples S phase to START and mitosis.
1993,
Pubmed King,
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.
1995,
Pubmed
,
Xenbase King,
Mitosis in transition.
1994,
Pubmed Klotzbücher,
The 'destruction box' of cyclin A allows B-type cyclins to be ubiquitinated, but not efficiently destroyed.
1996,
Pubmed Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase Lorca,
Cyclin A-Cys41 does not undergo cell cycle-dependent degradation in Xenopus extracts.
1992,
Pubmed
,
Xenbase Mahaffey,
Ubiquitination of full-length cyclin.
1995,
Pubmed
,
Xenbase Marks,
Cytoskeletal and DNA structure abnormalities result from bypass of requirement for the cdc10 start gene in the fission yeast Schizosaccharomyces pombe.
1992,
Pubmed Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Maundrell,
Thiamine-repressible expression vectors pREP and pRIP for fission yeast.
1993,
Pubmed Mitchison,
Growth in cell length in the fission yeast Schizosaccharomyces pombe.
1985,
Pubmed Moreno,
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
1991,
Pubmed Moreno,
Regulation of p34cdc2 protein kinase during mitosis.
1989,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Muzi Falconi,
cdc18+ regulates initiation of DNA replication in Schizosaccharomyces pombe.
1996,
Pubmed Nishitani,
p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast.
1995,
Pubmed Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed O'Donnell,
bimA encodes a member of the tetratricopeptide repeat family of proteins and is required for the completion of mitosis in Aspergillus nidulans.
1991,
Pubmed Ohkura,
The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
1989,
Pubmed Okazaki,
High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe.
1990,
Pubmed Rimmington,
Expression of N-terminally truncated cyclin B in the Drosophila larval brain leads to mitotic delay at late anaphase.
1994,
Pubmed Russell,
The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe.
1983,
Pubmed Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Sazer,
Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast.
1990,
Pubmed Sigrist,
Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.
1995,
Pubmed Simanis,
The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.
1986,
Pubmed Stewart,
Destruction of Xenopus cyclins A and B2, but not B1, requires binding to p34cdc2.
1994,
Pubmed
,
Xenbase Stone,
Mitotic regulation of protein phosphatases by the fission yeast sds22 protein.
1993,
Pubmed Stratmann,
Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition.
1996,
Pubmed Sudakin,
The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.
1995,
Pubmed Surana,
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
1993,
Pubmed Toda,
Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe.
1983,
Pubmed Tugendreich,
CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition.
1995,
Pubmed van der Velden,
Mitotic arrest caused by the amino terminus of Xenopus cyclin B2.
1993,
Pubmed
,
Xenbase Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase Watanabe,
Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs.
1991,
Pubmed
,
Xenbase Woods,
Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies.
1989,
Pubmed Wuarin,
Regulating S phase: CDKs, licensing and proteolysis.
1996,
Pubmed Yamamoto,
Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae.
1996,
Pubmed Yamamoto,
Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).
1996,
Pubmed Ye,
The NIMA protein kinase is hyperphosphorylated and activated downstream of p34cdc2/cyclin B: coordination of two mitosis promoting kinases.
1995,
Pubmed
