Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Calcium signaling is known to be associated with cytokinesis; however, the detailed spatio-temporal pattern of calcium dynamics has remained unclear. We have studied changes of intracellular free calcium in cleavage-stage Xenopus embryos using fluorescent calcium indicator dyes, mainly Calcium Green-1. Cleavage formation was followed by calcium transients that localized to cleavage furrows and propagated along the furrows as calcium waves. The calcium transients at the cleavage furrows were observed at each cleavage furrow at least until blastula stage. The velocity of the calcium waves at the first cleavage furrow was approximately 3 microns/s, which was much slower than that associated with fertilization/egg activation. These calcium waves traveled only along the cleavage furrows and not in the direction orthogonal to the furrows. These observations imply that there exists an intracellular calcium-releasing activity specifically associated with cleavage furrows. The calcium waves occurred in the absence of extracellular calcium and were inhibited in embryos injected with heparin an inositol 1,4,5-trisphosphate (InsP3) receptor antagonist. These results suggest that InsP3 receptor-mediated calcium mobilization plays an essential role in calcium wave formation at the cleavage furrows.
Bi,
Calcium-regulated exocytosis is required for cell membrane resealing.
1995, Pubmed
Bi,
Calcium-regulated exocytosis is required for cell membrane resealing.
1995,
Pubmed Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase Byers,
Membrane protein redistribution during Xenopus first cleavage.
1986,
Pubmed
,
Xenbase Cao,
Mechanism of the formation of contractile ring in dividing cultured animal cells. II. Cortical movement of microinjected actin filaments.
1990,
Pubmed Chang,
A localized elevation of cytosolic free calcium is associated with cytokinesis in the zebrafish embryo.
1995,
Pubmed Fluck,
Slow calcium waves accompany cytokinesis in medaka fish eggs.
1991,
Pubmed Furuichi,
Intracellular channels.
1994,
Pubmed Grandin,
Intracellular free calcium oscillates during cell division of Xenopus embryos.
1991,
Pubmed
,
Xenbase Han,
Reducing inositol lipid hydrolysis, Ins(1,4,5)P3 receptor availability, or Ca2+ gradients lengthens the duration of the cell cycle in Xenopus laevis blastomeres.
1992,
Pubmed
,
Xenbase Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase Hepler,
The role of calcium in cell division.
1994,
Pubmed Keating,
Intracellular free calcium oscillations in normal and cleavage-blocked embryos and artificially activated eggs of Xenopus laevis.
1994,
Pubmed
,
Xenbase Kubota,
Oscillation of intracellular free calcium in cleaving and cleavage-arrested embryos of Xenopus laevis.
1993,
Pubmed
,
Xenbase Kume,
The Xenopus IP3 receptor: structure, function, and localization in oocytes and eggs.
1993,
Pubmed
,
Xenbase McDougall,
Function and characteristics of repetitive calcium waves associated with meiosis.
1995,
Pubmed Mittal,
Visualization of myosin in living cells.
1987,
Pubmed Nuccitelli,
The sperm-induced Ca2+ wave following fertilization of the Xenopus egg requires the production of Ins(1, 4, 5)P3.
1993,
Pubmed
,
Xenbase Parys,
Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase Poenie,
Changes of free calcium levels with stages of the cell division cycle.
,
Pubmed Snow,
Calcium buffer injections delay cleavage in Xenopus laevis blastomeres.
1993,
Pubmed
,
Xenbase Stricker,
Time-lapse confocal imaging of calcium dynamics in starfish embryos.
1995,
Pubmed Sugiyama,
Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor: application to the studies on inflammatory cells.
1994,
Pubmed Sullivan,
Calcium mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function.
1993,
Pubmed
,
Xenbase Tombes,
Meiosis, egg activation, and nuclear envelope breakdown are differentially reliant on Ca2+, whereas germinal vesicle breakdown is Ca2+ independent in the mouse oocyte.
1992,
Pubmed Tsien,
Measurement of cytosolic free Ca2+ with quin2.
1989,
Pubmed Yamakita,
In vivo phosphorylation of regulatory light chain of myosin II during mitosis of cultured cells.
1994,
Pubmed
