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Mitotic cyclins are abruptly degraded at the end of mitosis by a cell-cycle-regulated ubiquitin-dependent proteolytic system. To understand how cyclin is recognized for ubiquitin conjugation, we have performed a mutagenic analysis of the destruction signal of mitotic cyclins. We demonstrate that an N-terminal cyclin B segment as short as 27 residues, containing the 9-amino-acid destruction box, is sufficient to destabilize a heterologous protein in mitotic Xenopus extracts. Each of the three highly conserved residues of the cyclin B destruction box is essential for ubiquitination and subsequent degradation. Although an intact destruction box is essential for the degradation of both A- and B-type cyclins, we find that the Xenopus cyclin A1 destruction box cannot functionally substitute for its B-type counterpart, because it does not contain the highly conserved asparagine necessary for cyclin B proteolysis. Physical analysis of ubiquitinated cyclin B intermediates demonstrates that multiple lysine residues function as ubiquitin acceptor sites, and mutagenic studies indicate that no single lysine residue is essential for cyclin B degradation. This study defines the key residues of the destruction box that target cyclin for ubiquitination and suggests there are important differences in the way in which A- and B-type cyclins are recognized by the cyclin ubiquitination machinery.
Amon,
Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle.
1994, Pubmed
Amon,
Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle.
1994,
Pubmed Aristarkhov,
E2-C, a cyclin-selective ubiquitin carrier protein required for the destruction of mitotic cyclins.
1996,
Pubmed Bachmair,
The degradation signal in a short-lived protein.
1989,
Pubmed Beers,
Utility of polyhistidine-tagged ubiquitin in the purification of ubiquitin-protein conjugates and as an affinity ligand for the purification of ubiquitin-specific hydrolases.
1993,
Pubmed Chau,
A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein.
1989,
Pubmed Deshaies,
The self-destructive personality of a cell cycle in transition.
1995,
Pubmed Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed Galan,
The yeast plasma membrane uracil permease is stabilized against stress induced degradation by a point mutation in a cyclin-like "destruction box".
1994,
Pubmed Gallant,
Identification of a novel vertebrate cyclin: cyclin B3 shares properties with both A- and B-type cyclins.
1994,
Pubmed Gallant,
Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells.
1992,
Pubmed
,
Xenbase Ghiara,
A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
1991,
Pubmed Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Glotzer,
Cell cycle. The only way out of mitosis.
1995,
Pubmed Hershko,
Methylated ubiquitin inhibits cyclin degradation in clam embryo extracts.
1991,
Pubmed Hershko,
Occurrence of a polyubiquitin structure in ubiquitin-protein conjugates.
1985,
Pubmed Hochstrasser,
The short-lived MAT alpha 2 transcriptional regulator is ubiquitinated in vivo.
1991,
Pubmed Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase Hunt,
The requirements for protein synthesis and degradation, and the control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo.
1992,
Pubmed Irniger,
Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast.
1995,
Pubmed Izumi,
Phosphorylation of Xenopus cyclins B1 and B2 is not required for cell cycle transitions.
1991,
Pubmed
,
Xenbase Kaplon,
The cellular content of Cdc25p, the Ras exchange factor in Saccharomyces cerevisiae, is regulated by destabilization through a cyclin destruction box.
1995,
Pubmed King,
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.
1995,
Pubmed
,
Xenbase King,
Mitosis in transition.
1994,
Pubmed Klotzbücher,
The 'destruction box' of cyclin A allows B-type cyclins to be ubiquitinated, but not efficiently destroyed.
1996,
Pubmed Kobayashi,
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
1992,
Pubmed
,
Xenbase Li,
Requirement for phosphorylation of cyclin B1 for Xenopus oocyte maturation.
1995,
Pubmed
,
Xenbase Lorca,
Cyclin A-Cys41 does not undergo cell cycle-dependent degradation in Xenopus extracts.
1992,
Pubmed
,
Xenbase Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase Luo,
Overexpression of a truncated cyclin B gene arrests Dictyostelium cell division during mitosis.
1994,
Pubmed Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Pines,
Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.
1991,
Pubmed Rimmington,
Expression of N-terminally truncated cyclin B in the Drosophila larval brain leads to mitotic delay at late anaphase.
1994,
Pubmed Scheffner,
The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53.
1993,
Pubmed Schwob,
The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae.
1994,
Pubmed Sigrist,
Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.
1995,
Pubmed Sokolik,
Ubiquitin conjugation to cytochromes c. Structure of the yeast iso-1 conjugate and possible recognition determinants.
1992,
Pubmed Sokolik,
The structures of ubiquitin conjugates of yeast Iso-2-cytochrome c.
1991,
Pubmed Stewart,
Destruction of Xenopus cyclins A and B2, but not B1, requires binding to p34cdc2.
1994,
Pubmed
,
Xenbase Studier,
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
1986,
Pubmed Sudakin,
The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.
1995,
Pubmed Surana,
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
1993,
Pubmed Treier,
Ubiquitin-dependent c-Jun degradation in vivo is mediated by the delta domain.
1994,
Pubmed Tugendreich,
CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition.
1995,
Pubmed van der Velden,
Mitotic arrest caused by the amino terminus of Xenopus cyclin B2.
1993,
Pubmed
,
Xenbase van der Velden,
Cell cycle-regulated degradation of Xenopus cyclin B2 requires binding to p34cdc2.
1994,
Pubmed
,
Xenbase Whitfield,
The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition.
1990,
Pubmed Yamamoto,
Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).
1996,
Pubmed Yamamoto,
Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae.
1996,
Pubmed Yu,
Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation.
1996,
Pubmed
,
Xenbase Zachariae,
TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast.
1996,
Pubmed
,
Xenbase
