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We have cloned and expressed a Ca(2+)-activated K+ channel beta-subunit from human brain. The open reading frame encodes a 191-amino acid protein possessing significant homology to a previously described subunit cloned from bovine muscle. The gene for this subunit is located on chromosome 5 at band q34 (hslo-beta). There is no evidence for alternative RNA splicing of this gene product. hslo-beta mRNA is abundantly expressed in smooth muscle, but expression levels are low in most other tissues, including brain. Brain subregions in which beta-subunit mRNA expression is relatively high are the hippocampus and corpus callosum. The coexpression of hslo-beta mRNA together with hslo-alpha subunits in either Xenopus oocytes or stably transfected HEK 293 cells give rise to Ca(2+)-activated potassium currents with a much increased calcium and/or voltage sensitivity. These data indicate that the beta-subunit shows a tissue distribution different to that of the alpha-subunit, and in many tissues there may be no association of alpha-subunits with beta-subunits. These beta-subunits can play a functional role in the regulation of neuronal excitability by tuning the Ca2+ and/or the voltage dependence of alpha-subunits.
Adelman,
Calcium-activated potassium channels expressed from cloned complementary DNAs.
1992,
Pubmed
,
Xenbase Atkinson,
A component of calcium-activated potassium channels encoded by the Drosophila slo locus.
1991,
Pubmed Bielefeldt,
Phosphorylation and dephosphorylation modulate a Ca(2+)-activated K+ channel in rat peptidergic nerve terminals.
1994,
Pubmed Butler,
mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels.
1993,
Pubmed
,
Xenbase Christie,
Heteropolymeric potassium channels expressed in Xenopus oocytes from cloned subunits.
1990,
Pubmed
,
Xenbase Chung,
Protein kinase activity closely associated with a reconstituted calcium-activated potassium channel.
1991,
Pubmed DiChiara,
Distinct effects of Ca2+ and voltage on the activation and deactivation of cloned Ca(2+)-activated K+ channels.
1995,
Pubmed
,
Xenbase Galland,
Chromosomal localization of FLT4, a novel receptor-type tyrosine kinase gene.
1992,
Pubmed Garcia-Calvo,
Purification and reconstitution of the high-conductance, calcium-activated potassium channel from tracheal smooth muscle.
1994,
Pubmed Gola,
Colocalization of active KCa channels and Ca2+ channels within Ca2+ domains in helix neurons.
1993,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Hicks,
Further evidence for clustering of human GABAA receptor subunit genes: localization of the alpha 6-subunit gene (GABRA6) to distal chromosome 5q by linkage analysis.
1994,
Pubmed Isacoff,
Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase Isom,
Auxiliary subunits of voltage-gated ion channels.
1994,
Pubmed Knaus,
Subunit composition of the high conductance calcium-activated potassium channel from smooth muscle, a representative of the mSlo and slowpoke family of potassium channels.
1994,
Pubmed Knaus,
Primary sequence and immunological characterization of beta-subunit of high conductance Ca(2+)-activated K+ channel from smooth muscle.
1994,
Pubmed Latorre,
Varieties of calcium-activated potassium channels.
1989,
Pubmed Maletic-Savatic,
Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro.
1995,
Pubmed Marty,
Potassium channels in cultured bovine adrenal chromaffin cells.
1985,
Pubmed Maruyama,
Quantification of Ca2+-activated K+ channels under hormonal control in pig pancreas acinar cells.
,
Pubmed McCormack,
Shaker K+ channel subunits from heteromultimeric channels with novel functional properties.
1990,
Pubmed
,
Xenbase McManus,
Functional role of the beta subunit of high conductance calcium-activated potassium channels.
1995,
Pubmed
,
Xenbase McManus,
Calcium-activated potassium channels: regulation by calcium.
1991,
Pubmed McPhalen,
Calcium-binding sites in proteins: a structural perspective.
1991,
Pubmed Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase Moczydlowski,
Gating kinetics of Ca2+-activated K+ channels from rat muscle incorporated into planar lipid bilayers. Evidence for two voltage-dependent Ca2+ binding reactions.
1983,
Pubmed Morales,
A novel beta subunit increases rate of inactivation of specific voltage-gated potassium channel alpha subunits.
1995,
Pubmed
,
Xenbase Pérez,
Reconstitution of expressed KCa channels from Xenopus oocytes to lipid bilayers.
1994,
Pubmed
,
Xenbase Pierce,
A mouse genomic library in the bacteriophage P1 cloning system: organization and characterization.
1992,
Pubmed Reinhart,
The contribution of both extracellular and intracellular calcium to the action of alpha-adrenergic agonists in perfused rat liver.
1984,
Pubmed Reinhart,
A family of calcium-dependent potassium channels from rat brain.
1989,
Pubmed Reinhart,
Modulation of calcium-activated potassium channels from rat brain by protein kinase A and phosphatase 2A.
1991,
Pubmed Reinhart,
Kinase and phosphatase activities intimately associated with a reconstituted calcium-dependent potassium channel.
1995,
Pubmed Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase Rhodes,
Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain.
1995,
Pubmed Roberts,
Colocalization of ion channels involved in frequency selectivity and synaptic transmission at presynaptic active zones of hair cells.
1990,
Pubmed Robitaille,
Functional colocalization of calcium and calcium-gated potassium channels in control of transmitter release.
1993,
Pubmed Robitaille,
Presynaptic calcium signals and transmitter release are modulated by calcium-activated potassium channels.
1992,
Pubmed Tseng-Crank,
Cloning, expression, and distribution of functionally distinct Ca(2+)-activated K+ channel isoforms from human brain.
1994,
Pubmed
,
Xenbase Wisgirda,
Functional dependence of Ca(2+)-activated K+ current on L- and N-type Ca2+ channels: differences between chicken sympathetic and parasympathetic neurons suggest different regulatory mechanisms.
1994,
Pubmed
