Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The lipocalinXlcpl1 expressed in the neural plate of Xenopus laevis embryos is a secreted retinaldehyde binding protein.
Lepperdinger G, Strobl B, Jilek A, Weber A, Thalhamer J, Flöckner H, Mollay C.
???displayArticle.abstract???
The cellular and structural properties and binding capabilities of a lipocalin expressed in the early neural plate of Xenopus laevis embryos and the adult choroid plexus have been investigated. It was found that this lipocalin, termed Xlcpl1, binds retinal at a nanomolar concentration, retinoic acid in the micromolar range, but does not show binding to retinol. Furthermore, this protein also binds D/L thyroxine. The Xlcpl1 cDNA was expressed in cell culture using the vaccinia virus expression system. In AtT20 cells, Xlcpl1 was secreted via the constitutive secretory pathway. We therefore assume that cpl1 binds retinaldehyde during the transport through the compartments of the secretory pathway that are considered to be the storage compartments of retinoids. Therefore, cpl1-expressing cells will secrete the precursors of active retinoids such as retinoic acid isomers. These retinoids may enter the cytosol by diffusion or receptor-controlled mechanisms, as has been shown for exogenously applied retinoids. Based on these data, it is suggested that cpl1 is an integral member of the retinoid signaling pathway and, therefore, it plays a key role in pattern formation in early embryonic development.
Barton,
A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
1987, Pubmed
Barton,
A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
1987,
Pubmed Cogan,
Binding affinities of retinol and related compounds to retinol binding proteins.
1976,
Pubmed Creech Kraft,
Temporal distribution, localization and metabolism of all-trans-retinol, didehydroretinol and all-trans-retinal during Xenopus development.
1994,
Pubmed
,
Xenbase Dekker,
Overexpression of a cellular retinoic acid binding protein (xCRABP) causes anteroposterior defects in developing Xenopus embryos.
1994,
Pubmed
,
Xenbase Durston,
Retinoic acid causes an anteroposterior transformation in the developing central nervous system.
1989,
Pubmed
,
Xenbase Falkner,
Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors.
1988,
Pubmed Fernandez,
An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis.
1994,
Pubmed Flower,
Structure and sequence relationships in the lipocalins and related proteins.
1993,
Pubmed Harland,
Neural induction in Xenopus.
1994,
Pubmed
,
Xenbase Huber,
Molecular structure of the bilin binding protein (BBP) from Pieris brassicae after refinement at 2.0 A resolution.
1987,
Pubmed Kato,
Immunohistochemical studies on the localization of cellular retinol-binding protein in rat testis and epididymis.
1985,
Pubmed Kraft,
Xenopus laevis: a model system for the study of embryonic retinoid metabolism. I. Embryonic metabolism of 9-cis- and all-trans-retinals and retinols to their corresponding acid forms.
1995,
Pubmed
,
Xenbase Kraft,
The retinoid X receptor ligand, 9-cis-retinoic acid, is a potential regulator of early Xenopus development.
1994,
Pubmed
,
Xenbase Langston,
Retinoic acid and homeobox gene regulation.
1994,
Pubmed Newcomer,
Retinoid-binding proteins: structural determinants important for function.
1995,
Pubmed Newcomer,
Structure of the epididymal retinoic acid binding protein at 2.1 A resolution.
1993,
Pubmed Newcomer,
X-ray crystallographic identification of a protein-binding site for both all-trans- and 9-cis-retinoic acid.
1993,
Pubmed Pappas,
Endogenous retinoids in rat epididymal tissue and rat and human spermatozoa.
1993,
Pubmed Porter,
Localization of cellular retinol-binding protein and cellular retinoic acid-binding protein in the rat testis and epididymis.
1985,
Pubmed Posch,
Biosynthesis of all-trans-retinoic acid from retinal. Recognition of retinal bound to cellular retinol binding protein (type I) as substrate by a purified cytosolic dehydrogenase.
1992,
Pubmed Posch,
Holocellular retinol binding protein as a substrate for microsomal retinal synthesis.
1991,
Pubmed Richter,
Gene expression in the embryonic nervous system of Xenopus laevis.
1988,
Pubmed
,
Xenbase Stunnenberg,
High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus.
1988,
Pubmed von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed Way,
Identification of a region in segment 1 of gelsolin critical for actin binding.
1990,
Pubmed
