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XB-ART-18098
FEBS Lett 1996 Jun 10;3881:59-65.
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Molecular determinants of external barium block in Shaker potassium channels.

Hurst RS, Toro L, Stefani E.


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Mutations in the outer pore region of Shaker K+ channels (T449 and D447) can influence external Ba2+ block. Substitution of T449 by A, V or Y differentially reduced Ba2+ block primarily by decreasing the blocking rate. Substitution of D447 by N resulted in a non-conducting channel with apparently normal gating currents. External Ba2+ can speed the OFF gating current of a different non-conducting mutant, W434F; this effect was markedly attenuated by the D447N substitution. These results suggest that D447 contributes to an external Ba2+ binding site while T449 imposes a barrier to the access of that site.

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???displayArticle.link??? FEBS Lett
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