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Cell
2005 May 20;1214:529-539. doi: 10.1016/j.cell.2005.03.009.
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Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity.
Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM.
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The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro.
Anantharaman,
Comparative genomics and evolution of proteins involved in RNA metabolism.
2002, Pubmed
Anantharaman,
Comparative genomics and evolution of proteins involved in RNA metabolism.
2002,
Pubmed Baldwin,
Cation binding to the integrin CD11b I domain and activation model assessment.
1998,
Pubmed Bateman,
The TROVE module: a common element in Telomerase, Ro and Vault ribonucleoproteins.
2003,
Pubmed Batey,
Structural and energetic analysis of RNA recognition by a universally conserved protein from the signal recognition particle.
2001,
Pubmed Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed Buyon,
Neonatal lupus syndromes.
2004,
Pubmed Carter,
The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease.
1971,
Pubmed Chen,
The Ro 60 kDa autoantigen: insights into cellular function and role in autoimmunity.
2004,
Pubmed Chen,
Ro ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation.
2000,
Pubmed Chen,
The Ro autoantigen binds misfolded U2 small nuclear RNAs and assists mammalian cell survival after UV irradiation.
2003,
Pubmed
,
Xenbase Cingolani,
Structure of importin-beta bound to the IBB domain of importin-alpha.
1999,
Pubmed Farris,
Y3 is the most conserved small RNA component of Ro ribonucleoprotein complexes in vertebrate species.
1995,
Pubmed
,
Xenbase Green,
Binding of the 60-kDa Ro autoantigen to Y RNAs: evidence for recognition in the major groove of a conserved helix.
1998,
Pubmed
,
Xenbase Groves,
The structure of the protein phosphatase 2A PR65/A subunit reveals the conformation of its 15 tandemly repeated HEAT motifs.
1999,
Pubmed Hendrick,
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
1981,
Pubmed Herschlag,
RNA chaperones and the RNA folding problem.
1995,
Pubmed Huber,
Three-dimensional structure of the armadillo repeat region of beta-catenin.
1997,
Pubmed Kickhoefer,
The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle.
2001,
Pubmed Kleywegt,
Model building and refinement practice.
1997,
Pubmed Macrae,
Ro's role in RNA reconnaissance.
2005,
Pubmed Maquat,
Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics.
2004,
Pubmed O'Brien,
A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.
1994,
Pubmed
,
Xenbase O'Brien,
Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins.
1993,
Pubmed
,
Xenbase Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed Otwinowski,
[20] Processing of X-ray diffraction data collected in oscillation mode.
2019,
Pubmed Parker,
The enzymes and control of eukaryotic mRNA turnover.
2004,
Pubmed Patel,
Cutaneous lupus erythematosus: a review.
2002,
Pubmed Pruijn,
Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes.
1991,
Pubmed Schroeder,
Strategies for RNA folding and assembly.
2004,
Pubmed Shi,
A misfolded form of 5S rRNA is complexed with the Ro and La autoantigens.
1996,
Pubmed
,
Xenbase Shimaoka,
Conformational regulation of integrin structure and function.
2002,
Pubmed Terwilliger,
Automated MAD and MIR structure solution.
1999,
Pubmed Van Horn,
Caenorhabditis elegans embryos contain only one major species of Ro RNP.
1995,
Pubmed
,
Xenbase Wang,
Modular recognition of RNA by a human pumilio-homology domain.
2002,
Pubmed
,
Xenbase Whittaker,
Distribution and evolution of von Willebrand/integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere.
2002,
Pubmed Wolin,
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
1984,
Pubmed Xue,
A lupus-like syndrome develops in mice lacking the Ro 60-kDa protein, a major lupus autoantigen.
2003,
Pubmed
