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During mitosis, microtubules not only grow fast, but also have a high rate of catastrophe. This is achieved in part by the activity of the MAP, XMAP215, which can stimulate the growth rate of microtubules without fully inhibiting the function of the catastrophe-kinesin XKCM1. We do not know whether this activity is particular to XMAP215, or is a general property of all MAPs. Here, we compare the activities of XMAP215 with the neuronal MAP tau, in opposing the destabilizing activity of the non-conventional kinesin XKCM1. We show that tau is a much more potent inhibitor of XKCM1 than XMAP215. Because tau completely suppresses XKCM1 activity, even at low concentrations, the combination of tau and XKCM1 is unable to generate mitotic microtubule dynamics.
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990, Pubmed,
Xenbase
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase Charrasse,
The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.
1998,
Pubmed
,
Xenbase Desai,
Kin I kinesins are microtubule-destabilizing enzymes.
1999,
Pubmed
,
Xenbase Drechsel,
Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau.
1992,
Pubmed Hirokawa,
Selective stabilization of tau in axons and microtubule-associated protein 2C in cell bodies and dendrites contributes to polarized localization of cytoskeletal proteins in mature neurons.
1996,
Pubmed Howard,
Dynamics and mechanics of the microtubule plus end.
2003,
Pubmed Kinoshita,
Reconstitution of physiological microtubule dynamics using purified components.
2001,
Pubmed
,
Xenbase Kinoshita,
XMAP215: a key component of the dynamic microtubule cytoskeleton.
2002,
Pubmed
,
Xenbase Lewis,
Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein.
1988,
Pubmed Mitchison,
Dynamic instability of microtubule growth.
,
Pubmed Mitchison,
Microtubule assembly nucleated by isolated centrosomes.
,
Pubmed Ohkura,
Dis1/TOG universal microtubule adaptors - one MAP for all?
2001,
Pubmed Tournebize,
Role of chromosomes in assembly of meiotic and mitotic spindles.
1997,
Pubmed
,
Xenbase van Breugel,
Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer.
2003,
Pubmed
,
Xenbase Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed Walker,
Dilution of individual microtubules observed in real time in vitro: evidence that cap size is small and independent of elongation rate.
1991,
Pubmed Weingarten,
A protein factor essential for microtubule assembly.
1975,
Pubmed West,
A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences.
1991,
Pubmed
