Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Estimating single-channel kinetic parameters from idealized patch-clamp data containing missed events.
Qin F, Auerbach A, Sachs F.
???displayArticle.abstract???
We present here a maximal likelihood algorithm for estimating single-channel kinetic parameters from idealized patch-clamp data. The algorithm takes into account missed events caused by limited time resolution of the recording system. Assuming a fixed dead time, we derive an explicit expression for the corrected transition rate matrix by generalizing the theory of Roux and Sauve (1985, Biophys. J. 48:149-158) to the case of multiple conductance levels. We use a variable metric optimizer with analytical derivatives for rapidly maximizing the likelihood. The algorithm is applicable to data containing substates and multiple identical or nonidentical channels. It allows multiple data sets obtained under different experimental conditions, e.g., concentration, voltage, and force, to be fit simultaneously. It also permits a variety of constraints on rate constants and provides standard errors for all estimates of model parameters. The algorithm has been tested extensively on a variety of kinetic models with both simulated and experimental data. It is very efficient and robust; rate constants for a multistate model can often be extracted in a processing time of approximately 1 min, largely independent of the starting values.
Albertsen,
Estimation of kinetic rate constants from multi-channel recordings by a direct fit of the time series.
1994, Pubmed
Albertsen,
Estimation of kinetic rate constants from multi-channel recordings by a direct fit of the time series.
1994,
Pubmed Auerbach,
A statistical analysis of acetylcholine receptor activation in Xenopus myocytes: stepwise versus concerted models of gating.
1993,
Pubmed
,
Xenbase Ball,
Ion-channel gating mechanisms: model identification and parameter estimation from single channel recordings.
1989,
Pubmed Blatz,
Quantitative description of three modes of activity of fast chloride channels from rat skeletal muscle.
1986,
Pubmed Blatz,
Correcting single channel data for missed events.
1986,
Pubmed Chay,
Kinetic modeling for the channel gating process from single channel patch clamp data.
1988,
Pubmed Chung,
Characterization of single channel currents using digital signal processing techniques based on Hidden Markov Models.
1990,
Pubmed Colquhoun,
On the stochastic properties of single ion channels.
1981,
Pubmed Colquhoun,
Relaxation and fluctuations of membrane currents that flow through drug-operated channels.
1977,
Pubmed Crouzy,
Yet another approach to the dwell-time omission problem of single-channel analysis.
1990,
Pubmed Draber,
Detection of jumps in single-channel data containing subconductance levels.
1994,
Pubmed Fredkin,
Bayesian restoration of single-channel patch clamp recordings.
1992,
Pubmed Hawkes,
Asymptotic distributions of apparent open times and shut times in a single channel record allowing for the omission of brief events.
1992,
Pubmed Horn,
Estimating kinetic constants from single channel data.
1983,
Pubmed Huang,
Gating kinetics of batrachotoxin-modified sodium channels in neuroblastoma cells determined from single-channel measurements.
1984,
Pubmed Läuger,
Internal motions in proteins and gating kinetics of ionic channels.
1988,
Pubmed Magleby,
Estimating kinetic parameters for single channels with simulation. A general method that resolves the missed event problem and accounts for noise.
1990,
Pubmed Magleby,
Calcium dependence of open and shut interval distributions from calcium-activated potassium channels in cultured rat muscle.
1983,
Pubmed McManus,
Accounting for the Ca(2+)-dependent kinetics of single large-conductance Ca(2+)-activated K+ channels in rat skeletal muscle.
1991,
Pubmed Milne,
Stochastic modelling of a single ion channel: an alternating renewal approach with application to limited time resolution.
1988,
Pubmed Roux,
A general solution to the time interval omission problem applied to single channel analysis.
1985,
Pubmed Sachs,
The automated analysis of data from single ionic channels.
1982,
Pubmed Vandenberg,
A sodium channel gating model based on single channel, macroscopic ionic, and gating currents in the squid giant axon.
1991,
Pubmed Weiss,
Voltage-dependent gating mechanism for single fast chloride channels from rat skeletal muscle.
1992,
Pubmed
