XB-ART-18873
EMBO J
1995 Dec 15;1424:6268-79. doi: 10.1002/j.1460-2075.1995.tb00317.x.
Show Gene links
Show Anatomy links
Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1.
Gawantka V, Delius H, Hirschfeld K, Blumenstock C, Niehrs C.
???displayArticle.abstract???
We have identified a novel homeobox gene, Xvent-1, that is differentially expressed in the ventral marginal zone of the early Xenopus gastrula. Evidence is presented from mRNA microinjection experiments for a role for this gene in dorsoventral patterning of mesoderm. First, Xvent-1 is induced by BMP-4, a gene known to be a key regulator of ventral mesoderm development. Second, Xvent-1 and the organizer-specific gene goosecoid are able to interact, directly or indirectly, in a cross-regulatory loop suppressing each other's expression, consistent with their mutually exclusive expression in the marginal zone. Third, microinjection of Xvent-1 mRNA ventralizes dorsal mesoderm. The results suggest that Xvent-1 functions in a ventral signaling pathway that maintains the ventral mesodermal state and antagonizes the Spemann organizer.
???displayArticle.pubmedLink??? 8557046
???displayArticle.pmcLink??? PMC394751
???displayArticle.link??? EMBO J
Species referenced: Xenopus
Genes referenced: gsc ventx1.2
References [+] :
Amaya, FGF signalling in the early specification of mesoderm in Xenopus. 1993, Pubmed , Xenbase
Arendt, Inversion of dorsoventral axis? 1994, Pubmed
Blitz, Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle. 1995, Pubmed , Xenbase
Blumberg, Organizer-specific homeobox genes in Xenopus laevis embryos. 1991, Pubmed , Xenbase
Candia, The expression pattern of Xenopus Mox-2 implies a role in initial mesodermal differentiation. 1995, Pubmed , Xenbase
Candia, Amino acid sequence of Mox-2 and comparison to its Xenopus and rat homologs. 1993, Pubmed , Xenbase
Cho, Overexpression of a homeodomain protein confers axis-forming activity to uncommitted Xenopus embryonic cells. 1991, Pubmed , Xenbase
Cho, Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid. 1991, Pubmed , Xenbase
Christian, Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus. 1993, Pubmed , Xenbase
Cribbs, Structural complexity and evolutionary conservation of the Drosophila homeotic gene proboscipedia. 1992, Pubmed
Dale, Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development. 1992, Pubmed , Xenbase
Dale, Regional specification within the mesoderm of early embryos of Xenopus laevis. 1987, Pubmed , Xenbase
Dale, Fate map for the 32-cell stage of Xenopus laevis. 1987, Pubmed , Xenbase
Dent, A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. 1989, Pubmed , Xenbase
Fainsod, On the function of BMP-4 in patterning the marginal zone of the Xenopus embryo. 1994, Pubmed , Xenbase
François, Xenopus chordin and Drosophila short gastrulation genes encode homologous proteins functioning in dorsal-ventral axis formation. 1995, Pubmed , Xenbase
Gont, Tail formation as a continuation of gastrulation: the multiple cell populations of the Xenopus tailbud derive from the late blastopore lip. 1993, Pubmed , Xenbase
Graff, Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. 1994, Pubmed , Xenbase
Halpern, Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. 1993, Pubmed
Harland, The transforming growth factor beta family and induction of the vertebrate mesoderm: bone morphogenetic proteins are ventral inducers. 1994, Pubmed , Xenbase
Harland, In situ hybridization: an improved whole-mount method for Xenopus embryos. 1991, Pubmed , Xenbase
Harvey, Microinjection of synthetic Xhox-1A homeobox mRNA disrupts somite formation in developing Xenopus embryos. 1988, Pubmed , Xenbase
Holley, A conserved system for dorsal-ventral patterning in insects and vertebrates involving sog and chordin. 1995, Pubmed , Xenbase
Jones, Inductive signals. Revolving vertebrates. 1995, Pubmed , Xenbase
Jones, DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction. 1992, Pubmed , Xenbase
Kao, The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos. 1988, Pubmed , Xenbase
Kao, Generation of body plan phenotypes in early embryogenesis. 1991, Pubmed , Xenbase
Keller, Cellular basis of amphibian gastrulation. 1992, Pubmed
Kimelman, Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction. 1992, Pubmed , Xenbase
Köster, Bone morphogenetic protein 4 (BMP-4), a member of the TGF-beta family, in early embryos of Xenopus laevis: analysis of mesoderm inducing activity. 1991, Pubmed , Xenbase
Kozak, An analysis of vertebrate mRNA sequences: intimations of translational control. 1991, Pubmed
Mohun, Cell type-specific activation of actin genes in the early amphibian embryo. , Pubmed , Xenbase
Moody, Fates of the blastomeres of the 32-cell-stage Xenopus embryo. 1987, Pubmed , Xenbase
Niehrs, Mesodermal patterning by a gradient of the vertebrate homeobox gene goosecoid. 1994, Pubmed , Xenbase
Niehrs, The homeobox gene goosecoid controls cell migration in Xenopus embryos. 1993, Pubmed , Xenbase
Niehrs, Ectopic expression of a homeobox gene changes cell fate in Xenopus embryos in a position-specific manner. 1991, Pubmed , Xenbase
Nishimatsu, Genes for bone morphogenetic proteins are differentially transcribed in early amphibian embryos. 1992, Pubmed , Xenbase
Northrop, Dorsal-ventral differences in Xcad-3 expression in response to FGF-mediated induction in Xenopus. 1994, Pubmed , Xenbase
Pannese, The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates and specifies anterior body regions. 1995, Pubmed , Xenbase
Ruiz i Altaba, Bimodal and graded expression of the Xenopus homeobox gene Xhox3 during embryonic development. 1989, Pubmed , Xenbase
Ruiz i Altaba, Involvement of the Xenopus homeobox gene Xhox3 in pattern formation along the anterior-posterior axis. 1989, Pubmed , Xenbase
Rupp, Xenopus embryos regulate the nuclear localization of XMyoD. 1994, Pubmed , Xenbase
Sanger, DNA sequencing with chain-terminating inhibitors. 1977, Pubmed
Sasai, Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. 1994, Pubmed , Xenbase
Scharf, Axis determination in eggs of Xenopus laevis: a critical period before first cleavage, identified by the common effects of cold, pressure and ultraviolet irradiation. 1983, Pubmed , Xenbase
Schmidt, Localized BMP-4 mediates dorsal/ventral patterning in the early Xenopus embryo. 1995, Pubmed , Xenbase
Sharpe, A homeobox-containing marker of posterior neural differentiation shows the importance of predetermination in neural induction. 1987, Pubmed , Xenbase
Simeone, Two vertebrate homeobox genes related to the Drosophila empty spiracles gene are expressed in the embryonic cerebral cortex. 1992, Pubmed
Sive, The frog prince-ss: a molecular formula for dorsoventral patterning in Xenopus. 1993, Pubmed , Xenbase
Slack, Embryonic induction. 1993, Pubmed , Xenbase
Smith, Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center. 1991, Pubmed , Xenbase
Smith, Dorsalization and neural induction: properties of the organizer in Xenopus laevis. 1983, Pubmed , Xenbase
Smith, Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. 1991, Pubmed , Xenbase
Steinbeisser, The role of gsc and BMP-4 in dorsal-ventral patterning of the marginal zone in Xenopus: a loss-of-function study using antisense RNA. 1995, Pubmed , Xenbase
Suzuki, A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo. 1994, Pubmed , Xenbase
Taira, Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus. 1994, Pubmed , Xenbase
Taira, The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos. 1992, Pubmed , Xenbase
von Dassow, Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene. 1993, Pubmed , Xenbase
Winklbauer, Mesodermal cell migration during Xenopus gastrulation. 1990, Pubmed , Xenbase
Witta, XIPOU 2, a noggin-inducible gene, has direct neuralizing activity. 1995, Pubmed , Xenbase
Zanetti, Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. 1985, Pubmed , Xenbase