Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1995 Dec 01;1423:5947-56. doi: 10.1002/j.1460-2075.1995.tb00283.x.
Show Gene links
Show Anatomy links
Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development.
Borden KL, Lally JM, Martin SR, O'Reilly NJ, Etkin LD, Freemont PS.
???displayArticle.abstract???
Xenopus nuclear factor XNF7, a maternally expressed protein, functions in patterning of the embryo. XNF7 contains a number of defined protein domains implicated in the regulation of some developmental processes. Among these is a tripartite motif comprising a zinc-binding RING finger and B-box domain next to a predicted alpha-helical coiled-coil domain. Interestingly, this motif is found in a variety of protein including several proto-oncoproteins. Here we describe the solution structure of the XNF7 B-box zinc-binding domain determined at physiological pH by 1H NMR methods. The B-box structure represents the first three-dimensional structure of this new motif and comprises a monomer have two beta-strands, two helical turns and three extended loop regions packed in a novel topology. The r.m.s. deviation for the best 18 structures is 1.15 A for backbone atoms and 1.94 A for all atoms. Structure calculations and biochemical data shows one zinc atom ligated in a Cys2-His2 tetrahedral arrangement. We have used mutant peptides to determine the metal ligation scheme which surprisingly shows that not all of the seven conserved cysteines/histidines in the B-box motif are involved in metal ligation. The B-box structure is not similar in tertiary fold to any other known zinc-binding motif.
Bellini,
A putative zinc-binding protein on lampbrush chromosome loops.
1993, Pubmed
Bellini,
A putative zinc-binding protein on lampbrush chromosome loops.
1993,
Pubmed Borden,
The solution structure of the RING finger domain from the acute promyelocytic leukaemia proto-oncoprotein PML.
1995,
Pubmed Borden,
Characterisation of a novel cysteine/histidine-rich metal binding domain from Xenopus nuclear factor XNF7.
1993,
Pubmed
,
Xenbase Brown,
Regulation of BRCA1.
,
Pubmed Campbell,
A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1.
1994,
Pubmed Chan,
Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen.
1991,
Pubmed de Thé,
The PML-RAR alpha fusion mRNA generated by the t(15;17) translocation in acute promyelocytic leukemia encodes a functionally altered RAR.
1991,
Pubmed Frankel,
Tat protein from human immunodeficiency virus forms a metal-linked dimer.
1988,
Pubmed Freemont,
The RING finger. A novel protein sequence motif related to the zinc finger.
1993,
Pubmed Freemont,
A novel cysteine-rich sequence motif.
1991,
Pubmed Georgiadis,
Crystallographic structure of the nitrogenase iron protein from Azotobacter vinelandii.
1992,
Pubmed Goddard,
Characterization of a zinc finger gene disrupted by the t(15;17) in acute promyelocytic leukemia.
1991,
Pubmed Harbury,
A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants.
1993,
Pubmed Inoue,
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
1993,
Pubmed Kakizuka,
Chromosomal translocation t(15;17) in human acute promyelocytic leukemia fuses RAR alpha with a novel putative transcription factor, PML.
1991,
Pubmed Karas,
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons.
1988,
Pubmed Kastner,
Structure, localization and transcriptional properties of two classes of retinoic acid receptor alpha fusion proteins in acute promyelocytic leukemia (APL): structural similarities with a new family of oncoproteins.
1992,
Pubmed
,
Xenbase Le Douarin,
The N-terminal part of TIF1, a putative mediator of the ligand-dependent activation function (AF-2) of nuclear receptors, is fused to B-raf in the oncogenic protein T18.
1995,
Pubmed Leonhardt,
Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC).
1994,
Pubmed Li,
Cytoplasmic retention of Xenopus nuclear factor 7 before the mid blastula transition uses a unique anchoring mechanism involving a retention domain and several phosphorylation sites.
1994,
Pubmed
,
Xenbase Lovering,
Identification and preliminary characterization of a protein motif related to the zinc finger.
1993,
Pubmed Marion,
Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.
1983,
Pubmed Michael,
Metal binding and folding properties of a minimalist Cys2His2 zinc finger peptide.
1992,
Pubmed Miki,
A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1.
1994,
Pubmed Miki,
Development of a highly efficient expression cDNA cloning system: application to oncogene isolation.
1991,
Pubmed Miller,
xlgv7: a maternal gene product localized in nuclei of the central nervous system in Xenopus laevis.
1989,
Pubmed
,
Xenbase Miller,
The nuclear-cytoplasmic distribution of the Xenopus nuclear factor, xnf7, coincides with its state of phosphorylation during early development.
1991,
Pubmed
,
Xenbase Neuhaus,
Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet.
1992,
Pubmed Nilges,
Determination of three-dimensional structures of proteins from interproton distance data by hybrid distance geometry-dynamical simulated annealing calculations.
1988,
Pubmed Reddy,
A unique bipartite cysteine-histidine motif defines a subfamily of potential zinc-finger proteins.
1991,
Pubmed
,
Xenbase Reddy,
A novel zinc finger coiled-coil domain in a family of nuclear proteins.
1992,
Pubmed Reddy,
The cloning and characterization of a maternally expressed novel zinc finger nuclear phosphoprotein (xnf7) in Xenopus laevis.
1991,
Pubmed
,
Xenbase Russell,
Multiple protein sequence alignment from tertiary structure comparison: assignment of global and residue confidence levels.
1992,
Pubmed Slobbe,
Ro ribonucleoprotein assembly in vitro. Identification of RNA-protein and protein-protein interactions.
1992,
Pubmed Takahashi,
Developmentally regulated expression of a human "finger"-containing gene encoded by the 5' half of the ret transforming gene.
1988,
Pubmed Wagner,
Protein structures in solution by nuclear magnetic resonance and distance geometry. The polypeptide fold of the basic pancreatic trypsin inhibitor determined using two different algorithms, DISGEO and DISMAN.
1987,
Pubmed Wishart,
Relationship between nuclear magnetic resonance chemical shift and protein secondary structure.
1991,
Pubmed
