Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We establish that the TATA binding protein (TBP) in the presence of TFIIA recognizes the TATA box in nucleosomal DNA dependent on the dissociation of the amino-terminal tails of the core histones from the nucleosome and the position of the TATA box within the nucleosome. We examine TBP/TFIIA access to the TATA box with this sequence placed in four distinct rotational frames with reference to the histone surface and at three distinct translational positions at the edge, side and dyad axis of the nucleosome. Under our experimental conditions, we find that the preferential translational position at which TBP/TFIIA can bind the TATA box is within linker DNA at the edge of the nucleosome and that binding is facilitated if contacts made by the amino-terminal tails of the histones with nucleosomal DNA are eliminated. TBP/TFIIA binding to DNA at the edge of the nucleosome occurs with the TATA box in all four rotational positions. This is indicative of TBP/TFIIA association directing the dissociation of the TATA box from the surface of the histone octamer.
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991, Pubmed
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991,
Pubmed Ausio,
Nucleosome core particle stability and conformational change. Effect of temperature, particle and NaCl concentrations, and crosslinking of histone H3 sulfhydryl groups.
1984,
Pubmed Ausio,
Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.
1989,
Pubmed Bashkin,
Structure of DNA in a nucleosome core at high salt concentration and at high temperature.
1993,
Pubmed
,
Xenbase Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed Cary,
Effect of acetylation on the binding of N-terminal peptides of histone H4 to DNA.
1982,
Pubmed Chen,
A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.
1994,
Pubmed Cormack,
The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells.
1992,
Pubmed Dong,
DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.
1990,
Pubmed Han,
Depletion of histone H4 and nucleosomes activates the PHO5 gene in Saccharomyces cerevisiae.
1988,
Pubmed Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase Hayes,
The histone core exerts a dominant constraint on the structure of DNA in a nucleosome.
1991,
Pubmed Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase Hayes,
Preferential and asymmetric interaction of linker histones with 5S DNA in the nucleosome.
1993,
Pubmed
,
Xenbase Hayes,
Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome".
1994,
Pubmed
,
Xenbase Hoffmann,
Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
1991,
Pubmed Horikoshi,
Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box.
1989,
Pubmed Horikoshi,
Transcription factor TFIID induces DNA bending upon binding to the TATA element.
1992,
Pubmed Imbalzano,
Facilitated binding of TATA-binding protein to nucleosomal DNA.
1994,
Pubmed Kahn,
DNA bending in transcription initiation.
1993,
Pubmed Kim,
Crystal structure of a yeast TBP/TATA-box complex.
1993,
Pubmed Kim,
Co-crystal structure of TBP recognizing the minor groove of a TATA element.
1993,
Pubmed Kim,
Involvement of the basic repeat domain of TATA-binding protein (TBP) in transcription by RNA polymerases I, II, and III.
1994,
Pubmed Kladde,
Positioned nucleosomes inhibit Dam methylation in vivo.
1994,
Pubmed Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase Li,
Translational positioning of a nucleosomal glucocorticoid response element modulates glucocorticoid receptor affinity.
1993,
Pubmed Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed Maldonado,
Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
1990,
Pubmed Matsui,
Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly.
1987,
Pubmed Meisterernst,
Recombinant yeast TFIID, a general transcription factor, mediates activation by the gene-specific factor USF in a chromatin assembly assay.
1990,
Pubmed
,
Xenbase Nightingale,
Methylation at CpG sequences does not influence histone H1 binding to a nucleosome including a Xenopus borealis 5 S rRNA gene.
1995,
Pubmed
,
Xenbase Nikolov,
Crystal structure of TFIID TATA-box binding protein.
1992,
Pubmed Patterton,
Nucleosomal location of the STE6 TATA box and Mat alpha 2p-mediated repression.
1994,
Pubmed Pennings,
Mobility of positioned nucleosomes on 5 S rDNA.
1991,
Pubmed Perlmann,
Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome.
1988,
Pubmed Piña,
Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter.
1990,
Pubmed Pruss,
Histone-DNA contacts in a nucleosome core containing a Xenopus 5S rRNA gene.
1993,
Pubmed
,
Xenbase Pruss,
Nucleosomal anatomy--where are the histones?
1995,
Pubmed Ranish,
Isolation of two genes that encode subunits of the yeast transcription factor IIA.
1992,
Pubmed Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase Richmond,
Studies of nucleosome structure.
1993,
Pubmed Ura,
A positive role for nucleosome mobility in the transcriptional activity of chromatin templates: restriction by linker histones.
1995,
Pubmed
,
Xenbase Vettese-Dadey,
Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.
1994,
Pubmed Wolffe,
Initiation of transcription on nucleosomal templates.
1989,
Pubmed Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase Yamashita,
Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.
1993,
Pubmed Yamashita,
Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB.
1992,
Pubmed
,
Xenbase
