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EMBO J
1995 Oct 16;1420:5073-84. doi: 10.1002/j.1460-2075.1995.tb00189.x.
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Programming of a repressed but committed chromatin structure during early development.
Prioleau MN, Buckle RS, Méchali M.
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The determination of chromatin for transcription during early development as well as the requirement for trans-acting factors during this period has been analysed in Xenopus. Basal transcription is repressed both during oogenesis and after the mid-blastula transition (MBT), and transactivators are required to relieve this repression. In contrast, transactivators cannot overcome the generalized transcriptional repression which occurs in embryos before MBT. However, they do bind to promoters leading to a repressed but preset chromatin structure. Experiments involving the pre-binding of TATA binding protein (TBP) or of the strong transactivator GAL4-VP16 further show that there is no limiting factor before the MBT, and that it is the recruitment and stabilization of the basal transcription machinery and not of transactivators which is repressed during early development. This multi-step process in gene activation, with activation of promoters temporally uncoupled from their commitment, may be of importance in the regulation of early embryonic events by providing molecular signposts for future determinations.
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974, Pubmed,
Xenbase
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974,
Pubmed
,
Xenbase Almouzni,
Constraints on transcriptional activator function contribute to transcriptional quiescence during early Xenopus embryogenesis.
1995,
Pubmed
,
Xenbase Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993,
Pubmed
,
Xenbase Bendig,
Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.
1984,
Pubmed
,
Xenbase Bentley,
Sequence requirements for premature termination of transcription in the human c-myc gene.
1988,
Pubmed
,
Xenbase Bienz,
A CCAAT box confers cell-type-specific regulation on the Xenopus hsp70 gene in oocytes.
1986,
Pubmed
,
Xenbase Burch,
Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
1983,
Pubmed Burton,
Expression in Escherichia coli: purification and properties of the yeast general transcription factor TFIID.
1991,
Pubmed Chasman,
Activation of yeast polymerase II transcription by herpesvirus VP16 and GAL4 derivatives in vitro.
1989,
Pubmed Dyson,
Chromatin structure around the c-myc gene in Burkitt lymphomas with upstream and downstream translocation points.
1985,
Pubmed Etkin,
The synthesis of authentic sea urchin transcriptional and translational products by sea urchin histone genes injected into Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase Felsenfeld,
Chromatin as an essential part of the transcriptional mechanism.
1992,
Pubmed Gimlich,
Early cellular interactions promote embryonic axis formation in Xenopus laevis.
1984,
Pubmed
,
Xenbase Green,
72-bp element contains a critical control region for SV40 late expression in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase Grosschedl,
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
1980,
Pubmed
,
Xenbase Gurdon,
All components required for the eventual activation of muscle-specific actin genes are localized in the subequatorial region of an uncleaved amphibian egg.
1985,
Pubmed
,
Xenbase Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed King,
Expression of the c-myc proto-oncogene during development of Xenopus laevis.
1986,
Pubmed
,
Xenbase Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase Lin,
GAL4 derivatives function alone and synergistically with mammalian activators in vitro.
1988,
Pubmed Lowenhaupt,
Chromatin structure in pre- and postblastula embryos of Drosophila.
1983,
Pubmed Mango,
Germ line c-myc is not down-regulated by loss or exclusion of activating factors in myc-induced macrophage tumors.
1989,
Pubmed Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase McKnight,
Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.
1980,
Pubmed
,
Xenbase Modak,
Regulation of Xenopus c-myc promoter activity in oocytes and embryos.
1993,
Pubmed
,
Xenbase Mohun,
Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Nishikura,
Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.
1986,
Pubmed
,
Xenbase Ovsenek,
Analysis of CCAAT box transcription factor binding activity during early Xenopus laevis embryogenesis.
1991,
Pubmed
,
Xenbase Ovsenek,
DNA sequence-specific binding activity of the heat-shock transcription factor is heat-inducible before the midblastula transition of early Xenopus development.
1990,
Pubmed
,
Xenbase Paranjape,
Role of chromatin structure in the regulation of transcription by RNA polymerase II.
1994,
Pubmed Perlmann,
Inhibition of chromatin assembly in Xenopus oocytes correlates with derepression of the mouse mammary tumor virus promoter.
1991,
Pubmed
,
Xenbase Prioleau,
Competition between chromatin and transcription complex assembly regulates gene expression during early development.
1994,
Pubmed
,
Xenbase Rougvie,
The RNA polymerase II molecule at the 5' end of the uninduced hsp70 gene of D. melanogaster is transcriptionally engaged.
1988,
Pubmed Rozek,
In vivo protein-DNA interactions at the c-jun promoter: preformed complexes mediate the UV response.
1993,
Pubmed Sealy,
Xenopus nucleoplasmin: egg vs. oocyte.
1986,
Pubmed
,
Xenbase Taylor,
Facilitated binding of GAL4 and heat shock factor to nucleosomal templates: differential function of DNA-binding domains.
1991,
Pubmed
,
Xenbase Taylor,
Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.
1986,
Pubmed
,
Xenbase Thomas,
Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.
1988,
Pubmed Tsukiyama,
ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.
1994,
Pubmed Vashee,
How do "Zn2 cys6" proteins distinguish between similar upstream activation sites? Comparison of the DNA-binding specificity of the GAL4 protein in vitro and in vivo.
1993,
Pubmed Vriz,
Differential expression of two Xenopus c-myc proto-oncogenes during development.
1989,
Pubmed
,
Xenbase Wallrath,
Architectural variations of inducible eukaryotic promoters: preset and remodeling chromatin structures.
1994,
Pubmed Wolffe,
Nucleosome positioning and modification: chromatin structures that potentiate transcription.
1994,
Pubmed
,
Xenbase Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase Workman,
Activation domains of stably bound GAL4 derivatives alleviate repression of promoters by nucleosomes.
1991,
Pubmed
,
Xenbase Workman,
Multiple functions of nucleosomes and regulatory factors in transcription.
1993,
Pubmed Workman,
Nucleosome core displacement in vitro via a metastable transcription factor-nucleosome complex.
1992,
Pubmed Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase Xu,
Different potential of cellular and viral activators of transcription revealed in oocytes and early embryos of Xenopus laevis.
1994,
Pubmed
,
Xenbase
