Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Insulin and insulin-like growth factor I (IGF-I) stimulate GLUT4 glucose transporter translocation in Xenopus oocytes.
Mora S, Kaliman P, Chillarón J, Testar X, Palacín M, Zorzano A.
???displayArticle.abstract???
1. The heterologous expression of glucose transporters GLUT4 and GLUT1 in Xenopus oocytes has been shown to cause a differential targeting of these glucose-carrier isoforms to cellular membranes and a distinct induction of glucose transport activity. In this study we have evaluated the effect of insulin and insulin-like growth factor I (IGF-I) on glucose uptake and glucose transporter distribution in Xenopus oocytes expressing mammalian GLUT4 and GLUT1 glucose carriers. 2. Insulin and IGF-I stimulated 2-deoxyglucose uptake in GLUT4-expressing oocytes, but not in GLUT1-expressing oocytes or in water-injected oocytes. The stimulatory effect of insulin and IGF-I on 2-deoxyglucose uptake in GLUT4-expressing oocytes occurred via activation of the IGF-I receptor. 3. Subcellular-fractionation studies indicated that insulin and IGF-I stimulated translocation of GLUT4 to the cell surface of the oocyte. 4. Incubation of intact oocytes with insulin stimulated phosphatidylinositol 3-kinase activity, an effect that was blocked by the additional presence of wortmannin. Furthermore, wortmannin totally abolished the insulin-induced stimulation of 2-deoxyglucose uptake in GLUT4-expressing oocytes. 5. In this study, both the insulin-induced GLUT4 carrier translocation and GLUT4-dependent insulin-stimulated glucose transport have been reconstituted in the Xenopus oocyte. These observations, together with the fact that wortmannin, as found in adipocytes, inhibits insulin-stimulated glucose transport in oocytes, suggest that the heterologous expression of GLUT4 in oocytes is a useful experimental model by which to study the cell biology of insulin-induced GLUT4 translocation.
Asano,
Domains responsible for the differential targeting of glucose transporter isoforms.
1992, Pubmed
Asano,
Domains responsible for the differential targeting of glucose transporter isoforms.
1992,
Pubmed Backer,
Phosphatidylinositol 3'-kinase is activated by association with IRS-1 during insulin stimulation.
1992,
Pubmed Baumert,
Synaptobrevin: an integral membrane protein of 18,000 daltons present in small synaptic vesicles of rat brain.
1989,
Pubmed Bertran,
Expression cloning of a human renal cDNA that induces high affinity transport of L-cystine shared with dibasic amino acids in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Bornemann,
Subcellular localization of GLUT4 in nonstimulated and insulin-stimulated soleus muscle of rat.
1992,
Pubmed Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Camps,
Effect of diabetes and fasting on GLUT-4 (muscle/fat) glucose-transporter expression in insulin-sensitive tissues. Heterogeneous response in heart, red and white muscle.
1992,
Pubmed Chuang,
Insulin receptor substrate 1 mediates insulin and insulin-like growth factor I-stimulated maturation of Xenopus oocytes.
1993,
Pubmed
,
Xenbase Clarke,
Inhibition of the translocation of GLUT1 and GLUT4 in 3T3-L1 cells by the phosphatidylinositol 3-kinase inhibitor, wortmannin.
1994,
Pubmed Cushman,
Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. Apparent translocation of intracellular transport systems to the plasma membrane.
1980,
Pubmed Endemann,
Phosphatidylinositol kinase or an associated protein is a substrate for the insulin receptor tyrosine kinase.
1990,
Pubmed Fischbarg,
Evidence that facilitative glucose transporters may fold as beta-barrels.
1993,
Pubmed
,
Xenbase Friedman,
Immunolocalization of glucose transporter GLUT4 within human skeletal muscle.
1991,
Pubmed Garcia,
Amino acid substitutions at tryptophan 388 and tryptophan 412 of the HepG2 (Glut1) glucose transporter inhibit transport activity and targeting to the plasma membrane in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Gawantka,
Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis.
1992,
Pubmed
,
Xenbase Giorgetti,
The insulin and insulin-like growth factor-I receptor substrate IRS-1 associates with and activates phosphatidylinositol 3-kinase in vitro.
1993,
Pubmed Gould,
Insulin-stimulated translocation of the HepG2/erythrocyte-type glucose transporter expressed in 3T3-L1 adipocytes.
1989,
Pubmed Gould,
Expression of human glucose transporters in Xenopus oocytes: kinetic characterization and substrate specificities of the erythrocyte, liver, and brain isoforms.
1991,
Pubmed
,
Xenbase Gould,
Expression of a functional glucose transporter in Xenopus oocytes.
1989,
Pubmed
,
Xenbase Gumà,
System A transport activity is stimulated in skeletal muscle in response to diabetes.
1992,
Pubmed Haney,
Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type.
1991,
Pubmed Hudson,
Isoform-specific subcellular targeting of glucose transporters in mouse fibroblasts.
1992,
Pubmed James,
Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.
1988,
Pubmed Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase Kanai,
Insulin-stimulated GLUT4 translocation is relevant to the phosphorylation of IRS-1 and the activity of PI3-kinase.
1993,
Pubmed Keller,
Functional expression of the human HepG2 and rat adipocyte glucose transporters in Xenopus oocytes. Comparison of kinetic parameters.
1989,
Pubmed
,
Xenbase Klip,
Acute and chronic signals controlling glucose transport in skeletal muscle.
1992,
Pubmed Kotliar,
Expression of the glucose transporter isoform GLUT 4 is insufficient to confer insulin-regulatable hexose uptake to cultured muscle cells.
1992,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Marshall,
Domains that confer intracellular sequestration of the Glut4 glucose transporter in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Merrall,
Mitogen-activated protein kinase (MAP kinase), MAP kinase kinase and c-Mos stimulate glucose transport in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Myers,
IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3'-kinase.
1993,
Pubmed Nakanishi,
Wortmannin, a microbial product inhibitor of myosin light chain kinase.
1992,
Pubmed Okada,
Essential role of phosphatidylinositol 3-kinase in insulin-induced glucose transport and antilipolysis in rat adipocytes. Studies with a selective inhibitor wortmannin.
1994,
Pubmed Piper,
Differential sorting of two glucose transporters expressed in insulin-sensitive cells.
1991,
Pubmed Ruderman,
Activation of phosphatidylinositol 3-kinase by insulin.
1990,
Pubmed Shibasaki,
Two glucose transporter isoforms are sorted differentially and are expressed in distinct cellular compartments.
1992,
Pubmed Slot,
Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat.
1991,
Pubmed Suzuki,
Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site.
1980,
Pubmed Thomas,
Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Vera,
Reconstitution of an insulin signaling pathway in Xenopus laevis oocytes: coexpression of a mammalian insulin receptor and three different mammalian hexose transporters.
1990,
Pubmed
,
Xenbase Vera,
Functional expression of mammalian glucose transporters in Xenopus laevis oocytes: evidence for cell-dependent insulin sensitivity.
1989,
Pubmed
,
Xenbase Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase Whitman,
Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation.
,
Pubmed Whitman,
Evidence for two distinct phosphatidylinositol kinases in fibroblasts. Implications for cellular regulation.
1987,
Pubmed Yano,
Inhibition of histamine secretion by wortmannin through the blockade of phosphatidylinositol 3-kinase in RBL-2H3 cells.
1993,
Pubmed
