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Members of the NAP/SET family of proteins interact specifically with B-type cyclins.
Kellogg DR, Kikuchi A, Fujii-Nakata T, Turck CW, Murray AW.
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Cyclin-dependent kinase complexes that contain the same catalytic subunit are able to induce different events at different times during the cell cycle, but the mechanisms by which they do so remain largely unknown. To address this problem, we have used affinity chromatography to identify proteins that bind specifically to mitotic cyclins, with the goal of finding proteins that interact with mitotic cyclins to carry out the events of mitosis. This approach has led to the identification of a 60-kD protein called NAP1 that interacts specifically with members of the cyclin B family. This interaction has been highly conserved during evolution: NAP1 in the Xenopus embryo interacts with cyclins B1 and B2, but not with cyclin A, and the S. cerevisiae homolog of NAP1 interacts with Clb2 but not with Clb3. Genetic experiments in budding yeast indicate that NAP1 plays an important role in the function of Clb2, while biochemical experiments demonstrate that purified NAP1 can be phosphorylated by cyclin B/p34cdc2 kinase complexes, but not by cyclin A/p34cdc2 kinase complexes. These results suggest that NAP1 is a protein involved in the specific functions of cyclin B/p34cdc2 kinase complexes. In addition to NAP1, we found a 43-kD protein in Xenopus that is homologous to NAP1 and also interacts specifically with B-type cyclins. This protein is the Xenopus homolog of the human SET protein, which was previously identified as part of a putative oncogenic fusion protein (Von Lindern et al., 1992).
Adachi,
Identification and characterization of SET, a nuclear phosphoprotein encoded by the translocation break point in acute undifferentiated leukemia.
1994, Pubmed
Adachi,
Identification and characterization of SET, a nuclear phosphoprotein encoded by the translocation break point in acute undifferentiated leukemia.
1994,
Pubmed Amon,
Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins.
1993,
Pubmed Anderson,
Processing of adenovirus 2-induced proteins.
1973,
Pubmed Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Brown,
Functional cDNA libraries from Drosophila embryos.
1988,
Pubmed Cao,
Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F.
1992,
Pubmed Devoto,
A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex.
1992,
Pubmed Dharmawardhane,
Compartmentalization and actin binding properties of ABP-50: the elongation factor-1 alpha of Dictyostelium.
1991,
Pubmed Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase Epstein,
CLB5: a novel B cyclin from budding yeast with a role in S phase.
1992,
Pubmed Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed Feaver,
Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK.
1994,
Pubmed Fisher,
A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase.
1994,
Pubmed Fitch,
Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.
1992,
Pubmed Fujii-Nakata,
Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity.
1992,
Pubmed Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gibbons,
A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library.
1991,
Pubmed Giordano,
A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
1989,
Pubmed Hartwell,
Genetic control of the cell division cycle in yeast.
1974,
Pubmed Ishimi,
Rapid purification of nucleosome assembly protein (AP-I) and production of monoclonal antibodies against it.
1985,
Pubmed Ishimi,
Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro.
1991,
Pubmed
,
Xenbase Ishimi,
Purification and initial characterization of a protein which facilitates assembly of nucleosome-like structure from mammalian cells.
1984,
Pubmed
,
Xenbase Kaffman,
Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85.
1994,
Pubmed Kato,
Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4.
1993,
Pubmed Kellogg,
Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies.
1992,
Pubmed Kilmartin,
A spacer protein in the Saccharomyces cerevisiae spindle poly body whose transcript is cell cycle-regulated.
1993,
Pubmed Koff,
Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.
1991,
Pubmed
,
Xenbase Kühne,
A new pair of B-type cyclins from Saccharomyces cerevisiae that function early in the cell cycle.
1993,
Pubmed Leiss,
Association of cyclin-bound p34cdc2 with subcellular structures in xenopus eggs.
1992,
Pubmed
,
Xenbase Lenstra,
Cloning and sequencing of Plasmodium falciparum DNA fragments containing repetitive regions potentially coding for histidine-rich proteins: identification of two overlapping reading frames.
1987,
Pubmed Léopold,
An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins.
1991,
Pubmed Lew,
Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.
1991,
Pubmed Lew,
Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins.
1993,
Pubmed Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase Mendenhall,
An inhibitor of p34CDC28 protein kinase activity from Saccharomyces cerevisiae.
1993,
Pubmed Meyerson,
A family of human cdc2-related protein kinases.
1992,
Pubmed Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase Morgan,
Principles of CDK regulation.
1995,
Pubmed Nasmyth,
Control of the yeast cell cycle by the Cdc28 protein kinase.
1993,
Pubmed Nevins,
E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.
1992,
Pubmed Nigg,
The substrates of the cdc2 kinase.
1991,
Pubmed Norbury,
Animal cell cycles and their control.
1992,
Pubmed Nurse,
Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe.
1980,
Pubmed Peeper,
A- and B-type cyclins differentially modulate substrate specificity of cyclin-cdk complexes.
1993,
Pubmed Peter,
Mitogen-activated protein kinases phosphorylate nuclear lamins and display sequence specificity overlapping that of mitotic protein kinase p34cdc2.
1992,
Pubmed Philpott,
E2F and its developmental regulation in Xenopus laevis.
1994,
Pubmed
,
Xenbase Pines,
Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
1987,
Pubmed
,
Xenbase Pines,
Cyclins and cyclin-dependent kinases: take your partners.
1993,
Pubmed Pines,
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
1990,
Pubmed Plyte,
Glycogen synthase kinase-3: functions in oncogenesis and development.
1992,
Pubmed Pringle,
Immunofluorescence methods for yeast.
1991,
Pubmed Reed,
The role of p34 kinases in the G1 to S-phase transition.
1992,
Pubmed Richardson,
Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2.
1992,
Pubmed Roy,
The MO15 cell cycle kinase is associated with the TFIIH transcription-DNA repair factor.
1994,
Pubmed Schwob,
CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae.
1993,
Pubmed Shiina,
Microtubule severing by elongation factor 1 alpha.
1994,
Pubmed
,
Xenbase Shirodkar,
The transcription factor E2F interacts with the retinoblastoma product and a p107-cyclin A complex in a cell cycle-regulated manner.
1992,
Pubmed Simon,
Molecular characterization of hNRP, a cDNA encoding a human nucleosome-assembly-protein-I-related gene product involved in the induction of cell proliferation.
1994,
Pubmed Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase Surana,
The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae.
1991,
Pubmed Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Thiele,
Elongation factor 1 alpha from Saccharomyces cerevisiae. Rapid large-scale purification and molecular characterization.
1985,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase von Lindern,
Can, a putative oncogene associated with myeloid leukemogenesis, may be activated by fusion of its 3' half to different genes: characterization of the set gene.
1992,
Pubmed Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
,
Xenbase Xiong,
Human D-type cyclin.
1991,
Pubmed Zhang,
Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes.
1993,
Pubmed
