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Shigella flexneri is a Gram-negative bacterial pathogen that can grow directly in the cytoplasm of infected host cells and uses a form of actin-based motility for intra- and intercellular spread. Moving intracellular bacteria are associated with a polarized "comet tail" composed of actin filaments. IcsA, a 120-kDa outer membrane protein necessary for actin-based motility, is located at a single pole on the surface of the organism, at the junction with the actin tail. Here, we demonstrate that stable expression of IcsA on the surface of Escherichia coli is sufficient to allow actin-dependent movement of E. coli in cytoplasmic extracts, at rates comparable to the movement of S. flexneri in infected cells. Thus, IcsA is the sole Shigella-specific factor required for actin-based motility. Continuous protein synthesis and polarized distribution of the protein are not necessary for actin tail formation or movement. Listeria monocytogenes is an unrelated bacterial pathogen that exhibits similar actin-based intracytoplasmic motility. Actin filament dynamics in the comet tails associated with the two different organisms are essentially identical, which indicates that they have independently evolved mechanisms to interact with the same components of the host cytoskeleton.
Akiyama,
SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro.
1990, Pubmed
Akiyama,
SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro.
1990,
Pubmed Allaoui,
icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread.
1992,
Pubmed Bernardini,
Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin.
1989,
Pubmed Dabiri,
Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly.
1990,
Pubmed d'Hauteville,
Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri.
1992,
Pubmed Domann,
A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin.
1992,
Pubmed Forscher,
Novel form of growth cone motility involving site-directed actin filament assembly.
1992,
Pubmed Goldberg,
Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.
1993,
Pubmed Goldberg,
Regulation of surface presentation of IcsA, a Shigella protein essential to intracellular movement and spread, is growth phase dependent.
1994,
Pubmed Homma,
Structural genes for flagellar hook-associated proteins in Salmonella typhimurium.
1985,
Pubmed Hovde,
Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I.
1988,
Pubmed Kadurugamuwa,
Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.
1991,
Pubmed Kocks,
Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly.
1993,
Pubmed Kocks,
L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein.
1992,
Pubmed Lett,
virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence.
1989,
Pubmed Makino,
A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in S. flexneri 2a.
1986,
Pubmed Meissner,
Bacteriophage lambda cloning system for the construction of directional cDNA libraries.
1987,
Pubmed Mounier,
Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocytelike cell line Caco-2.
1990,
Pubmed Nakata,
The absence of a surface protease, OmpT, determines the intercellular spreading ability of Shigella: the relationship between the ompT and kcpA loci.
1993,
Pubmed Nanavati,
Dynamics of actin and alpha-actinin in the tails of Listeria monocytogenes in infected PtK2 cells.
1994,
Pubmed Ogawa,
Cinemicrographic study of tissue cell cultures infected with Shigella flexneri.
1968,
Pubmed Pál,
Intracellular spread of Shigella flexneri associated with the kcpA locus and a 140-kilodalton protein.
1989,
Pubmed Prévost,
Unipolar reorganization of F-actin layer at bacterial division and bundling of actin filaments by plastin correlate with movement of Shigella flexneri within HeLa cells.
1992,
Pubmed Sandlin,
Avirulence of rough mutants of Shigella flexneri: requirement of O antigen for correct unipolar localization of IcsA in the bacterial outer membrane.
1995,
Pubmed Sanger,
Host cell actin assembly is necessary and likely to provide the propulsive force for intracellular movement of Listeria monocytogenes.
1992,
Pubmed Sansonetti,
Cadherin expression is required for the spread of Shigella flexneri between epithelial cells.
1994,
Pubmed Sansonetti,
Involvement of a plasmid in the invasive ability of Shigella flexneri.
1982,
Pubmed Sansonetti,
OmpB (osmo-regulation) and icsA (cell-to-cell spread) mutants of Shigella flexneri: vaccine candidates and probes to study the pathogenesis of shigellosis.
1991,
Pubmed Theriot,
Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts.
1994,
Pubmed
,
Xenbase Theriot,
Actin microfilament dynamics in locomoting cells.
1991,
Pubmed Theriot,
The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization.
1992,
Pubmed Tilney,
Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes.
1989,
Pubmed Tilney,
Actin filament nucleation by the bacterial pathogen, Listeria monocytogenes.
1990,
Pubmed Venkatesan,
Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus.
1992,
Pubmed
