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???displayArticle.abstract??? Eg5, a member of the bimC subfamily of kinesin-like microtubule motor proteins, localizes to spindle microtubules in mitosis but not to interphase microtubules. We investigated the molecular basis for spindle localization by transient transfection of Xenopus A6 cells with myc-tagged derivatives of Eg5. Expressed at constitutively high levels from a cytomegalovirus promoter, mycEg5 protein is cytoplasmic throughout interphase, begins to bind microtubules in early prophase, and remains localized to spindle and/or midbody microtubules through mitosis to the end of telophase. Both N- and C-terminal regions of Eg5 are required for this cell-cycle-regulated targeting. Eg5 also contains within its C-terminal domain a sequence conserved among bimC subfamily proteins that includes a potential p34cdc2 phosphorylation site. We show that mutation of a single threonine (T937) within this site to nonphosphorylatable alanine abolishes localization of the mutant protein to the spindle, whereas mutation of T937 to serine preserves spindle localization. We hypothesize that phosphorylation of Eg5 may regulate its localization to the spindle in the cell cycle.
Clark-Lewis,
Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase.
1991, Pubmed
Clark-Lewis,
Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase.
1991,
Pubmed Cole,
A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos.
1994,
Pubmed
,
Xenbase Connell-Crowley,
Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro.
1993,
Pubmed
,
Xenbase Crews,
Erks: their fifteen minutes has arrived.
1992,
Pubmed
,
Xenbase Endow,
Genetic approaches to molecular motors.
1992,
Pubmed Enos,
Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans.
1990,
Pubmed Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase Goodson,
Molecular phylogeny of the kinesin family of microtubule motor proteins.
1994,
Pubmed Gould,
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
1991,
Pubmed Hackney,
Kinesin undergoes a 9 S to 6 S conformational transition.
1992,
Pubmed Hagan,
Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast.
1992,
Pubmed Hagan,
Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene.
1990,
Pubmed Heck,
The kinesin-like protein KLP61F is essential for mitosis in Drosophila.
1993,
Pubmed Houliston,
The kinesin-related protein Eg5 associates with both interphase and spindle microtubules during Xenopus early development.
1994,
Pubmed
,
Xenbase Hoyt,
Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
1992,
Pubmed Hoyt,
Cellular roles of kinesin and related proteins.
1994,
Pubmed L'Allemain,
Growth factor-induced activation of a kinase activity which causes regulatory phosphorylation of p42/microtubule-associated protein kinase.
1992,
Pubmed Le Guellec,
Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein.
1991,
Pubmed
,
Xenbase Levin,
Association of catalytic and regulatory subunits of cyclic AMP-dependent protein kinase requires a negatively charged side group at a conserved threonine.
1990,
Pubmed Liao,
Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein.
1994,
Pubmed Moreno,
Substrates for p34cdc2: in vivo veritas?
1990,
Pubmed Navone,
Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.
1992,
Pubmed O'Connell,
Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a KAR3-related kinesin-like protein in Aspergillus nidulans.
1993,
Pubmed Page,
Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein.
1994,
Pubmed Roof,
Kinesin-related proteins required for assembly of the mitotic spindle.
1992,
Pubmed Sawin,
Mitotic spindle organization by a plus-end-directed microtubule motor.
1992,
Pubmed
,
Xenbase Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase Sawin,
Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules.
1994,
Pubmed
,
Xenbase Sawin,
Motor proteins in cell division.
1991,
Pubmed Sawin,
Meiosis, mitosis and microtubule motors.
1993,
Pubmed
,
Xenbase Schroer,
Structure, function and regulation of cytoplasmic dynein.
1994,
Pubmed Smith,
Xenopus cell lines.
1991,
Pubmed
,
Xenbase Toyoshima,
Kinectin, a major kinesin-binding protein on ER.
1992,
Pubmed Trybus,
Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength.
1984,
Pubmed Vulliet,
Identification of a novel proline-directed serine/threonine protein kinase in rat pheochromocytoma.
1989,
Pubmed
