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Modulation by zinc ions of native rat and recombinant human inhibitory glycine receptors.
Laube B, Kuhse J, Rundström N, Kirsch J, Schmieden V, Betz H.
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1. The effect of the divalent cation Zn2+ on inhibitory glycine receptor (GlyR) currents was investigated in rat embryonic spinal cord neurons and Xenopus oocytes expressing recombinant GlyRs. 2. In cultured spinal neurons, Zn2+ potentiated glycine-induced whole-cell currents about 3-fold when applied extracellularly at concentrations of 0.5-10 microM. In contrast, higher concentrations (> 100 microM) of Zn2+ decreased the glycine response. 3. A similar biphasic modulation of glycine-induced currents by Zn2+ was also found with recombinant homo- and hetero-oligomeric GlyRs generated in Xenopus oocytes. Dose-response analysis showed that both the potentiating and inhibitory effects of Zn2+ result from changes in apparent agonist affinity. 4. Analysis of chimeric constructs of the GlyR alpha 1- and beta-subunits revealed that the positive and negative modulatory effects of Zn2+ are mediated by different regions of the alpha 1-subunit. 5. Our data indicate the existence of distinct high- and low-affinity Zn2+ binding sites on the ligand-binding alpha-subunits of the GlyR. These sites may be implicated in the regulation of synaptic efficacy within glycinergic pathways.
Assaf,
Release of endogenous Zn2+ from brain tissue during activity.
, Pubmed
Assaf,
Release of endogenous Zn2+ from brain tissue during activity.
,
Pubmed Betz,
Structure and function of inhibitory glycine receptors.
1992,
Pubmed Draguhn,
Functional and molecular distinction between recombinant rat GABAA receptor subtypes by Zn2+.
1990,
Pubmed Grenningloh,
Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes.
1990,
Pubmed
,
Xenbase Grenningloh,
Cloning and expression of the 58 kd beta subunit of the inhibitory glycine receptor.
1990,
Pubmed
,
Xenbase Hoch,
Primary cultures of mouse spinal cord express the neonatal isoform of the inhibitory glycine receptor.
1989,
Pubmed Kirsch,
Gephyrin antisense oligonucleotides prevent glycine receptor clustering in spinal neurons.
,
Pubmed Korn,
Long-term potentiation of inhibitory circuits and synapses in the central nervous system.
1992,
Pubmed Kuhse,
Assembly of the inhibitory glycine receptor: identification of amino acid sequence motifs governing subunit stoichiometry.
1993,
Pubmed
,
Xenbase Kuryatov,
Mutational analysis of the glycine-binding site of the NMDA receptor: structural similarity with bacterial amino acid-binding proteins.
1994,
Pubmed
,
Xenbase Matzenbach,
Structural analysis of mouse glycine receptor alpha subunit genes. Identification and chromosomal localization of a novel variant.
1994,
Pubmed Pribilla,
The atypical M2 segment of the beta subunit confers picrotoxinin resistance to inhibitory glycine receptor channels.
1992,
Pubmed Schmieden,
Mutation of glycine receptor subunit creates beta-alanine receptor responsive to GABA.
1993,
Pubmed
,
Xenbase Schmieden,
Functional expression in Xenopus oocytes of the strychnine binding 48 kd subunit of the glycine receptor.
1989,
Pubmed
,
Xenbase Smart,
Modulation of inhibitory and excitatory amino acid receptor ion channels by zinc.
1994,
Pubmed Vallee,
Zinc coordination, function, and structure of zinc enzymes and other proteins.
1990,
Pubmed
