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Mol Cell Biol
1995 Mar 01;153:1422-30. doi: 10.1128/MCB.15.3.1422.
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Cloning and characterization of a Xenopus poly(A) polymerase.
Gebauer F, Richter JD.
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During oocyte maturation and early embryogenesis in Xenopus laevis, the translation of several mRNAs is regulated by cytoplasmic poly(A) elongation, a reaction catalyzed by poly(A) polymerase (PAP). We have cloned, sequenced, and examined several biochemical properties of a Xenopus PAP. This protein is 87% identical to the amino-terminal portion of bovine PAP, which catalyzes the nuclear polyadenylation reaction, but lacks a large region of the corresponding carboxy terminus, which contains the nuclear localization signal. When injected into oocytes, the Xenopus PAP remains concentrated in the cytoplasm, suggesting that it is a specifically cytoplasmic enzyme. Oocytes contain several PAP mRNA-related transcripts, and the levels of at least the one encoding the putative cytoplasmic enzyme are relatively constant in oocytes and early embryos but decline after blastulation. When expressed in bacteria and purified by affinity and MonoQ-Sepharose chromatography, the protein has enzymatic activity and adds poly(A) to a model substrate. Importantly, affinity-purified antibodies directed against Xenopus PAP inhibit cytoplasmic polyadenylation in egg extracts. These data suggest that the PAP described here could participate in cytoplasmic polyadenylation during Xenopus oocyte maturation.
Bilger,
Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements.
1994,
Pubmed
,
Xenbase Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Cao,
Identification of the gene for an Escherichia coli poly(A) polymerase.
1992,
Pubmed Dworkin,
Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis.
1985,
Pubmed
,
Xenbase Edmonds,
Polyadenylate polymerases.
1990,
Pubmed Egrie,
Changes in poly(adenylic acid) polymerase activity during sea urchin embryogenesis.
1979,
Pubmed Fox,
Polyadenylation of maternal mRNA during oocyte maturation: poly(A) addition in vitro requires a regulated RNA binding activity and a poly(A) polymerase.
1992,
Pubmed Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Gebauer,
Cloning and characterization of a Xenopus poly(A) polymerase.
1995,
Pubmed
,
Xenbase Gershon,
Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus.
1991,
Pubmed Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Huarte,
Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA.
1987,
Pubmed Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase Kormanec,
Simple and reliable determination of radioactive precursors incorporation into nucleic acids in vitro.
1993,
Pubmed Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase Lingner,
Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae.
1991,
Pubmed McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Niessing,
Evidence for the existence of two different poly(A) polymerases and a novel ribohomopolymer polymerase in rat liver nuclei.
1974,
Pubmed Olmsted,
Analysis of cytoskeletal structures using blot-purified monospecific antibodies.
1986,
Pubmed Paris,
Maturation-specific polyadenylation and translational control: diversity of cytoplasmic polyadenylation elements, influence of poly(A) tail size, and formation of stable polyadenylation complexes.
1990,
Pubmed
,
Xenbase Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase Pellicer,
Characterization of two poly(A) polymerases from cultured hamster fibroblasts.
1978,
Pubmed Raabe,
Poly(A) polymerase contains multiple functional domains.
1994,
Pubmed Raabe,
Primary structure and expression of bovine poly(A) polymerase.
1991,
Pubmed Richter,
Translational control in development: a perspective.
1993,
Pubmed
,
Xenbase Rosenthal,
Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.
1983,
Pubmed Ryner,
Multiple forms of poly(A) polymerases purified from HeLa cells function in specific mRNA 3'-end formation.
1989,
Pubmed Sachs,
Poly(A) tail metabolism and function in eucaryotes.
1993,
Pubmed Sallés,
Isolation of novel murine maternal mRNAs regulated by cytoplasmic polyadenylation.
1992,
Pubmed
,
Xenbase Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Simon,
Translational control by poly(A) elongation during Xenopus development: differential repression and enhancement by a novel cytoplasmic polyadenylation element.
1992,
Pubmed
,
Xenbase Simon,
Further analysis of cytoplasmic polyadenylation in Xenopus embryos and identification of embryonic cytoplasmic polyadenylation element-binding proteins.
1994,
Pubmed
,
Xenbase Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed Stetler,
Comparison of cytosolic and nuclear poly(A) polymerases from rat liver and a hepatoma: structural and immunological properties and response to NI-type protein kinases.
1985,
Pubmed Stetler,
Structurally and immunologically distinct poly(A) polymerases in rat liver. Occurrence of a tumor-type enzyme in normal liver.
1984,
Pubmed Thuresson,
Multiple forms of poly(A) polymerases in human cells.
1994,
Pubmed Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed Wahle,
Isolation and expression of cDNA clones encoding mammalian poly(A) polymerase.
1991,
Pubmed Wahle,
The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
1992,
Pubmed Wahle,
The end of the message: 3'-end processing leading to polyadenylated messenger RNA.
1992,
Pubmed Wormington,
Poly(A) and translation: development control.
1993,
Pubmed
,
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