Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway.
Braddock M, Muckenthaler M, White MR, Thorburn AM, Sommerville J, Kingsman AJ, Kingsman SM.
???displayArticle.abstract???
The translation of a capped, polyadenylated RNA after injection into the nucleus of Xenopus oocytes occurs only if the RNA contains an intron. A single point mutation in the splice donor site prevents translation. Intron-less RNA is exported efficiently to the cytoplasm and is held, undegraded, in a translationally inert state for several days. Translation can be activated by treating the oocytes with progesterone or by injecting antibodies that bind the FRGY2 class of messenger RNA binding proteins, p56 and p60, but these antibodies are only effective if delivered to the nucleus. Inhibitors of casein kinase II also activate translation whereas phosphatase inhibitors block progesterone-mediated activation of translation. These data suggest the presence of an RNA handling pathway in the nucleus of Xenopus oocytes which is regulated by casein kinase type II phosphorylation and which directs transcripts to be sequestered by p56/p60 or by closely related proteins. This pathway can be bypassed if the RNA contains an intron and it can be reversed by progesterone treatment. These data may have implications for understanding translational control during early development.
Arrigo,
Rev is necessary for translation but not cytoplasmic accumulation of HIV-1 vif, vpr, and env/vpu 2 RNAs.
1991, Pubmed
Arrigo,
Rev is necessary for translation but not cytoplasmic accumulation of HIV-1 vif, vpr, and env/vpu 2 RNAs.
1991,
Pubmed Baulieu,
Steroid-induced meiotic division in Xenopus laevis oocytes: surface and calcium.
1978,
Pubmed
,
Xenbase Bendig,
Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.
1984,
Pubmed
,
Xenbase Braddock,
A nuclear translational block imposed by the HIV-1 U3 region is relieved by the Tat-TAR interaction.
1990,
Pubmed
,
Xenbase Braddock,
HIV-1 TAT "activates" presynthesized RNA in the nucleus.
1989,
Pubmed
,
Xenbase Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed Capco,
Localized protein synthesis during oogenesis of Xenopus laevis: analysis by in situ translation.
1982,
Pubmed
,
Xenbase Chang,
Regulation by HIV Rev depends upon recognition of splice sites.
1989,
Pubmed Cohen,
Okadaic acid: a new probe for the study of cellular regulation.
1990,
Pubmed Cummings,
Multiple modifications in the phosphoproteins bound to stored messenger RNA in Xenopus oocytes.
1989,
Pubmed
,
Xenbase Cummings,
Protein kinase activity associated with stored messenger ribonucleoprotein particles of Xenopus oocytes.
1988,
Pubmed
,
Xenbase D'Agostino,
The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs.
1992,
Pubmed Dargemont,
Export of mRNA from microinjected nuclei of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase Darnbrough,
Identification in Xenopus laevis of a class of oocyte-specific proteins bound to messenger RNA.
1981,
Pubmed
,
Xenbase Dearsly,
Identification of a 60-kDa phosphoprotein that binds stored messenger RNA of Xenopus oocytes.
1985,
Pubmed
,
Xenbase de la Peña,
Enhancement of mRNA nuclear transport by promoter elements.
1987,
Pubmed
,
Xenbase Deschamps,
Purification of two thermostable components of messenger ribonucleoprotein particles (mRNPs) from Xenopus laevis oocytes, belonging to a novel class of RNA-binding proteins.
1991,
Pubmed
,
Xenbase Deschamps,
mRNP4, a major mRNA-binding protein from Xenopus oocytes is identical to transcription factor FRG Y2.
1992,
Pubmed
,
Xenbase de Wet,
Firefly luciferase gene: structure and expression in mammalian cells.
1987,
Pubmed Emerman,
The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization.
1989,
Pubmed Ford,
A method for enucleating oocytes of Xenopus laevis.
1977,
Pubmed
,
Xenbase Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed Green,
Biochemical mechanisms of constitutive and regulated pre-mRNA splicing.
1991,
Pubmed Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase Guddat,
Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes.
1990,
Pubmed
,
Xenbase Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed Hadzopoulou-Cladaras,
The rev (trs/art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via a cis-acting sequence in the env region.
1989,
Pubmed Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase Hammarskjöld,
Regulation of human immunodeficiency virus env expression by the rev gene product.
1989,
Pubmed Jackson,
Do the poly(A) tail and 3' untranslated region control mRNA translation?
1990,
Pubmed Johnston,
Autoradiography using storage phosphor technology.
1990,
Pubmed Kick,
Phosphorylation of a 60 kDa polypeptide from Xenopus oocytes blocks messenger RNA translation.
1987,
Pubmed
,
Xenbase Krek,
Casein kinase II is a predominantly nuclear enzyme.
1992,
Pubmed Kwon,
Proteins homologous to the Xenopus germ cell-specific RNA-binding proteins p54/p56 are temporally expressed in mouse male germ cells.
1993,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lawrence,
The HIV-1 Rev protein: a model system for coupled RNA transport and translation.
1991,
Pubmed Legrain,
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
1989,
Pubmed Malim,
The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.
1989,
Pubmed Marello,
Binding of Xenopus oocyte masking proteins to mRNA sequences.
1992,
Pubmed
,
Xenbase McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Merriam,
Progesterone-induced maturational events in oocytes of Xenopus laevis. I. Continuous necessity for diffusible calcium and magnesium.
1971,
Pubmed
,
Xenbase Murray,
Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos.
1991,
Pubmed
,
Xenbase Murray,
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
1992,
Pubmed
,
Xenbase Paris,
Maturation-specific polyadenylation and translational control: diversity of cytoplasmic polyadenylation elements, influence of poly(A) tail size, and formation of stable polyadenylation complexes.
1990,
Pubmed
,
Xenbase Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase Ranjan,
Masking mRNA from translation in somatic cells.
1993,
Pubmed
,
Xenbase Richter,
Translational control during early development.
1991,
Pubmed
,
Xenbase Richter,
Reversible inhibition of translation by Xenopus oocyte-specific proteins.
,
Pubmed
,
Xenbase Ruderman,
Modulations of histone messenger RNA during the early development of Xenopus laevis.
1979,
Pubmed
,
Xenbase Scheer,
Injected histone antibodies interfere with transcription of lampbrush chromosome loops in oocytes of Pleurodeles.
1979,
Pubmed Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Sommerville,
RNA-binding phosphoproteins and the regulation of maternal mRNA in Xenopus.
1990,
Pubmed
,
Xenbase Sommerville,
RNA-binding proteins: masking proteins revealed.
1992,
Pubmed
,
Xenbase Sowden,
Multiple cooperative interactions constrain BPV-1 E2 dependent activation of transcription.
1989,
Pubmed Tafuri,
Dual roles for transcription and translation factors in the RNA storage particles of Xenopus oocytes.
1993,
Pubmed
,
Xenbase Tafuri,
Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation.
1990,
Pubmed
,
Xenbase Tafuri,
DNA binding, multimerization, and transcription stimulation by the Xenopus Y box proteins in vitro.
1992,
Pubmed
,
Xenbase Tafuri,
Selective recruitment of masked maternal mRNA from messenger ribonucleoprotein particles containing FRGY2 (mRNP4).
1993,
Pubmed
,
Xenbase Tobian,
tRNA nuclear transport: defining the critical regions of human tRNAimet by point mutagenesis.
1985,
Pubmed
,
Xenbase Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase Wolffe,
The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man.
1992,
Pubmed Woodland,
Utilization of stored mRNA in Xenopus embryos and its replacement by newly synthesized transcripts: histone H1 synthesis using interspecies hybrids.
1979,
Pubmed
,
Xenbase Zandomeni,
Inhibitory effect of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole on a protein kinase.
1984,
Pubmed Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
